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Method for quickly improving biological adhesiveness of laser patterned PDMS surface

A bio-adhesion and patterning technology is applied in the field of laser patterning PDMS surface to rapidly improve bio-adhesion and improve the bio-adhesion of PDMS surface. Adaptability, the effect of improving bioadhesion

Inactive Publication Date: 2016-12-21
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production cycle is long, and a mask with a pattern is required, and a silicon plate after photolithography is used as a template, PDMS is poured, and then demolded, and finally related surface modification is required, and the process is very complicated.

Method used

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  • Method for quickly improving biological adhesiveness of laser patterned PDMS surface
  • Method for quickly improving biological adhesiveness of laser patterned PDMS surface
  • Method for quickly improving biological adhesiveness of laser patterned PDMS surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] like figure 1 As shown, a laser patterning PDMS surface rapid improvement method of bio-adhesion, the PDMS surface will form a microstructure after laser action, providing a good environment for cell attachment growth;

[0038] The microstructure can immobilize proteins, cell growth factors, enzymes and nucleic acids.

[0039] The laser (1) generates a high-energy pulsed laser beam, and after the high-energy pulsed laser beam passes through the reflector (4), it is focused to the surface of the PDMS (7) through a convex lens (6). Under the action of energy and impact force, photothermal action and photochemical action occur on the surface of PDMS (7), and instantaneous vaporization or splashing, thereby forming microstructures on the surface of PDMS (7).

[0040] Specifically, PDMS is placed on the three-dimensional micro-working platform, and the vertical movement of the three-dimensional micro-working platform (8) is controlled by the computer (3) and the motion con...

Embodiment 2

[0042] like figure 1 As shown, a method for rapidly improving bioadhesion on the surface of laser patterned PDMS, through chemical modification or structural modification of the PDMS surface after laser action, thereby improving bioadhesion;

[0043] The laser (1) generates a high-energy pulsed laser beam, and after the high-energy pulsed laser beam passes through the reflector (4), it is focused to the surface of the PDMS (7) through a convex lens (6). Under the action of energy and impact force, the surface of PDMS(7) undergoes chemical or structural modification, thereby improving bioadhesion.

[0044] Specifically, PDMS is placed on the three-dimensional micro-working platform, and the vertical movement of the three-dimensional micro-working platform (8) is controlled by the computer (3) and the motion control system (2) to adjust the distance between the PDMS (7) surface and the laser focus. distance; through the computer (3) and the motion control system (2) to control...

Embodiment 3

[0046] like figure 1 As shown, a method of laser patterning PDMS surface to rapidly improve bioadhesion,

[0047] (1) PDMS substrate production: PDMS prepolymer and curing agent are mixed at a ratio of 10:1, and fully stirred for 10 minutes; the mixture is poured into the mold; because more air is mixed during the stirring, a vacuum pump is required Carry out vacuum pumping for half an hour; then put the mixture into a horizontal drying oven, set the temperature at 65 degrees Celsius, and accelerate curing for 4 hours to obtain a PDMS substrate; take out the PDMS substrate and seal it up.

[0048] (2) The laser is an ultraviolet pulsed laser with a wavelength of 355nm. When the laser is turned on, the laser (1) produces a high-energy pulsed laser beam. After the high-energy pulsed laser beam passes through the reflector (4), it is focused to PDMS (7) by a convex lens (6). ) surface, under the action of high energy and impact force brought by the high-energy pulsed laser beam...

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Abstract

The invention discloses a method for quickly improving biological adhesiveness of a laser patterned PDMS surface. A microstructure can be formed on the PDMS surface after laser action, so that a good environment is provided for cell adhesion and growth; or the PDMS surface after laser action is chemically modified or structurally modified, so that biological adhesiveness is improved; the microstructure can fix protein, cell growth factors, enzyme and nucleic acid; a laser device generates high-energy pulse laser beams which focus to the PDMS surface through a convex lens after going through a reflection mirror, and under action of high energy and impact force caused by radiation of the high-energy pulse laser beams, the PDMS surface occurs photothermal action and photochemical action and is instantly vaporized or splashes so as to form the microstructure on the PDMS surface. Laser action is utilized for material surface modification or forming the surface microstructure, modification step of the PDMS surface is simplified without having influence on a non-modified area surface, and the PDMS surface is patterned, so that preparation efficiency is improved greatly.

Description

technical field [0001] The invention relates to a technology for improving bioadhesion on the surface of PDMS, in particular to a method for rapidly improving bioadhesion on the surface of laser patterned PDMS, which belongs to the field of laser surface modification. Background technique [0002] PDMS (polydimethylsiloxane) has good characteristics such as easy injection molding, biocompatibility, light transmission, gas permeability, thermal stability and non-toxicity. It is not only used for processing conventional microfluidic chips, It is also gradually used as a structural material for space cell culture chips. However, unmodified PDMS is prone to non-specific adsorption of proteins or small hydrophobic molecules, which is not conducive to cell adhesion and proliferation. Therefore, it is necessary to modify the surface of PDMS in order to be better used for cell surface attachment culture and cell proliferation research. [0003] Currently, biomolecular modification...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B29C71/04B29C59/16
CPCB29C71/04B29C59/16
Inventor 郭钟宁洪文生邓宇张永康黄志刚麦文豪江树镇刘桂贤
Owner GUANGDONG UNIV OF TECH
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