Preparation method of injectable self-adaptive natural hydrogel adhesive

A natural water and adhesive technology, applied in surgical adhesives, applications, pharmaceutical formulations, etc., can solve the problems of difficult operation, uncoordinated local deformation, high mechanical hardness, etc., and achieve good bioadhesion and bacteriostasis. The effect of anti-inflammatory action

Active Publication Date: 2021-08-13
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no clinically effective method for repairing damaged lung tissue. The main reasons are as follows: (1) The lung tissue cannot be sutured with surgical needles and threads, because it will cause new air leakage points; (2) The lung tissue is soft and dynamic. The contraction-expansion entity, the mechanical hardness of the existing medical adhesive is too large, and the adhesion on the surface of the lung tissue cannot adapt to the dynamic change of the lung surface deformation, resulting in uncoordinated local deformation and excessive strain, which may cause Additional lung function damage; (3) The cost of thoracotomy is high, the patient is damaged greatly, and the operation is difficult. Even with minimally invasive surgery, the wound is still much larger than that of acupuncture or catheter

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This embodiment includes the following steps:

[0021] Step 1: Prepare a solvent with a volume ratio of 1:1 between deionized water and dimethyl sulfoxide, and dissolve 1.0g of gelatin into 40mL of solvent at 40°C to prepare a 2.5% gelatin solution; mix 2mM, 3mM or 4mM 4 -Carboxyphenylboronic acid or 4-carboxy-2-fluorophenylboronic acid was dissolved in 20mL solvent, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was added thereto in an equimolar ratio Salt) and N-hydroxysuccinimide, mix and let stand for 30 minutes, then mix the two solutions, adjust the pH of the solution to 5 with 1mol / L sodium hydroxide solution, and stir and react at 40°C for 1 day, Dialyze the solution in deionized water with a dialysis bag, change the water three times a day, freeze-dry after 5 days of dialysis, and obtain gelatin-phenylboronic acid or gelatin-fluorophenylboronic acid with degrees of substitution of 12%, 26% and 40%, respectively. ;

[0022] Step 2: Prepare a s...

Embodiment 2

[0026] This embodiment includes the following steps:

[0027] Step 1: Prepare a solvent with a volume ratio of 1:1 between deionized water and N,N-dimethylformamide, dissolve 1.0 g of sodium hyaluronate with a molecular weight of 90 kDa in 100 mL of solvent, and prepare 1% hyaluronic acid solution; to which was added 1.25mM, 3mM or 5mM 3-aminophenylboronic acid, and equimolar 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and N-hydroxysuccinate Imide, adjust the pH of the solution to 6 with 0.5mol / L sodium hydroxide solution, stir and react at room temperature for 1 day, then dialyze the solution in deionized water with a dialysis bag, change the water four times a day, and dialyze after 6 days Freeze-drying can obtain hyaluronic acid-phenylboronic acid with a substitution degree of 15%, 27% or 41%;

[0028] Step 2: Prepare a solvent with a volume ratio of 2:1 between deionized water and N,N-dimethylformamide, dissolve 1.0 g of sodium hyaluronate with a molecula...

Embodiment 3

[0032] This embodiment includes the following steps:

[0033] Step 1: Prepare a solvent with a volume ratio of deionized water and N,N-dimethylformamide of 3:2, dissolve 1.0 g of sodium hyaluronate with a molecular weight of 90 kDa in 100 mL of solvent, and prepare 3% hyaluronic acid solution; to which was added 1.25mM, 3mM or 5mM 3-aminophenylboronic acid, and equimolar 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and N-hydroxy For succinimide, use 1mol / L sodium hydroxide solution to adjust the pH of the solution to 5, stir and react at room temperature for 1 day, then dialyze the solution in deionized water with a dialysis bag, change the water three times a day, and freeze it after 5 days of dialysis After drying, hyaluronic acid-phenylboronic acid with a substitution degree of 15%, 27% or 41% can be obtained;

[0034] Step 2: Prepare a solvent with a volume ratio of deionized water to dimethyl sulfoxide of 2:1, dissolve 1.0g of gelatin into 40mL of solvent...

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Abstract

The invention provides a preparation method of an injectable self-adaptive natural hydrogel adhesive. The preparation method comprises the following steps: firstly, synthesizing gelatin-phenylboronic acid from carboxyl phenylboronic acid and gelatin through an amidation reaction, or synthesizing polysaccharide-phenylboronic acid from amino phenylboronic acid and natural polysaccharide; synthesizing gelatin-dopamine or polysaccharide-dopamine from dopamine and gelatin or natural polysaccharide through an amidation reaction; and dissolving the gelatin-phenylboronic acid / polysaccharide-phenylboronic acid in a phosphate buffer solution, dissolving the gelatin-dopamine / polysaccharide-dopamine in a phosphate buffer solution containing natural polyphenol substances, and uniformly mixing two obtained solutions to realize a spontaneous esterification reaction of phenylboronic acid and polyphenol so as to generate reversible boric acid ester bonds, thereby finally preparing the injectable self-adaptive natural hydrogel adhesive. The hydrogel adhesive disclosed by the invention has good biological adhesion and antibacterial and anti-inflammatory effects, and is attached to the surface of a tissue to play a repairing role; and the hydrogel adhesive has good mechanical self-adaptive performance and can be matched with deformation and frequency of different dynamic tissues.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method of an injectable self-adaptive natural hydrogel adhesive for repairing lung damage. Background technique [0002] Pneumothorax (pneumothorax) refers to the gas into the pleural cavity, resulting in a state of pneumothorax, known as pneumothorax. The lung tissue and visceral pleura are usually ruptured due to lung disease or external force, or the tiny emphysema bubbles near the lung surface are ruptured, and the air in the lungs and bronchi escapes into the pleural cavity. Generally speaking, those caused by chest wall or lung trauma are called traumatic pneumothorax; those caused by spontaneous rupture of lung tissue due to disease are called "spontaneous pneumothorax". The incidence of traumatic pneumothorax accounts for 15% to 50% in blunt trauma and 30% to 87.6% in penetrating trauma. Mostly because the lung is punctured by the broken end of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00C08H1/00
CPCC08J3/075A61L24/0031A61L24/0015A61L24/08A61L24/104C08J2389/00A61L2400/04A61L2400/06A61L2300/404A61L2300/41A61L2300/412A61L2300/216A61L2300/602
Inventor 魏钊贾渊博徐峰冯锦腾
Owner XI AN JIAOTONG UNIV
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