Method for preparing biological sensor by nano CaCO3 fixing biological molecules

A biosensor and biomolecule technology is applied in the field of nano-CaCO3 immobilizing biomolecules to prepare biosensors, and achieves high economic benefits, high sensitivity and good reproducibility.

Inactive Publication Date: 2007-04-11
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nano-CaCO 3 It has good biocompatibility and high specific surface area, which has very favorable conditions for the immobilization of biomolecules, but so far nano-CaCO 3 There have never been experiments and applications in biosensors. If nano-CaCO 3 In the preparation of biosensors, it is expected to construct bioactive membranes with excellent performance and biosensors with high stability and high sensitivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation of H by fixing hemoglobin 2 o 2 sensor

[0017] Step 1: Polish the base electrode to a mirror surface on a gauze dipped in an aqueous solution of aluminum oxide, then ultrasonically wash it with double distilled water for 5 minutes, and dry it.

[0018] Step 2: Prepare nano-CaCO with a concentration of 5 mg / ml with double distilled water 3 colloidal solution. Nano-CaCO 3 The particle size is 5-100nm.

[0019] Step 3: prepare 15 mg / ml hemoglobin solution with 0.01 mol / l phosphate buffer solution.

[0020] Step 4: Mix the colloid solution and hemoglobin solution in equal volumes (hemoglobin and nano-CaCO 3 The mass ratio is 3:1), shake gently to form a mixture.

[0021] Step 5: Add a chitosan solution with a concentration of 2 mg / ml to the mixed solution, and the volume ratio of the chitosan solution to the mixed solution is 1:6. Take 15μl of the mixed solution and evenly drop it on an area of ​​10mm 2 The surface of the glassy carbon elect...

Embodiment 2

[0023] Example 2: Preparation of glucose oxidase sensor

[0024] Step 1: Put the disk platinum electrode (area is 3mm 2 ) was polished to a mirror surface on gauze dipped in an aqueous solution of aluminum oxide, then ultrasonically washed with twice distilled water for 5 minutes, and dried.

[0025] Step 2: Prepare nano-CaCO with a concentration of 2 mg / ml with twice distilled water 3 colloidal solution. Nano-CaCO 3 The particle size is 5-100nm.

[0026] Step 3: Prepare a glucose oxidase solution with a concentration of 2 mg / ml.

[0027] Step 4: Using Glucose Oxidase and Nano-CaCO 3 The mass ratio is 2:1 and mixed to form a mixed solution.

[0028] Step 5: Take 45 μl of the mixture and evenly drop-coat it on the surface of the disc platinum electrode. After about 90% of the solvent is evaporated, put the electrode in the saturated vapor of glutaraldehyde for 15 minutes, and then put it in the refrigerator to refrigerate. That is, based on nano-CaCO 3 -Glucose sensor o...

Embodiment 3

[0030] Example 3: Preparation of polyphenol oxidase sensor

[0031] Step 1: Prepare 2 mg / ml nano-CaCO with twice distilled water 3 colloidal solution. Nano-CaCO 3 The particle size is 5-100nm.

[0032] Step 2: Prepare a polyphenol oxidase solution with a concentration of 4 mg / ml.

[0033] Step 3: Place the disk glassy carbon electrode (with an area of ​​7mm 2 ) was polished to a mirror surface on gauze dipped in an aqueous solution of aluminum oxide, then ultrasonically washed with twice distilled water for 5 minutes, and dried.

[0034] Step 4: Using polyphenol oxidase and nano-CaCO 3 The mass ratio is 1:1 and mixed to form a mixed solution.

[0035] Step 5: Take 20 μl of the mixed solution and evenly drop-coat it on the surface of the disk glassy carbon electrode. After about 85% of the solvent is evaporated, put the electrode in the saturated vapor of glutaraldehyde for 15 minutes, and then put it in the refrigerator to refrigerate. That is, based on nano-CaCO 3 - P...

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Abstract

The process of fixing protein or other biological molecule onto CaCO3 to prepare biosensor as nanometer inorganic carrier includes compounding colloid solution with CaCO3 and re-distilled water, adding biological molecule into the colloid solution to form mixed solution, dropping the mixed solution onto the surface of substrate electrode, evaporating 80-95 % of the solvent, and cross-linking with glutaraldehyde vapor to prepare the nanometer CaCO3-biological molecule composite biological film as the said biosensor. Thus prepared biosensor has high stability, high sensitivity, high repeatability and low needed biological molecule amount, and may be use widely in medicine, food, environment detection and other fields.

Description

technical field [0001] The invention belongs to the use of inorganic material nano CaCO 3 A method for preparing a biosensor for immobilizing biomolecules such as proteins on a carrier. Background technique [0002] Biosensors are analytical tools or systems composed of immobilized biological materials (including enzymes, antibodies, microorganisms and other biologically active substances) in close contact with appropriate conversion devices. It uses the affinity of biologically active substances, such as the molecular recognition function of enzyme-substrate, enzyme-prosthetic group, antigen-antibody, hormone-receptor, etc., and can selectively measure the other side. called molecular recognition elements. Due to the specific recognition function of biologically active substances, biosensors have high selectivity and can be directly applied to complex samples for detection. Biosensors have been widely valued for their high sensitivity, high selectivity, integration of se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N27/30C12Q1/00
Inventor 薛怀国朱明娟单丹王善霞
Owner YANGZHOU UNIV
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