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Preparation method of drug and protein sustained-release alginate hybrid gel

The technology of alginate and hybrid gel is applied in the field of functional materials and biological materials, and can solve the problems of difficult protein slow release in hydrogel, strong hydrophilicity of calcium alginate gel, difficult protein slow release, etc. Achieving the effects of diverse forms, good biocompatibility and good application prospects

Inactive Publication Date: 2013-04-17
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the deficiencies in the prior art, the technical problems to be solved by the present invention are calcium alginate gels with strong hydrophilicity, sudden release of drugs in hydrogels, difficulty in sustained release of proteins in hydrogels, etc.
[0008] The technical scheme of the present invention to solve the problems of strong hydrophilicity of the calcium alginate gel, sudden release of the drug in the hydrogel, and difficulty in the sustained release of the protein in the hydrogel is to design a drug and protein slow-release alginate hybrid gel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. A preparation method of doxorubicin sustained-release phosphoric acid / calcium alginate hybrid gel film

[0021] a) Prepare a mixed aqueous solution of 0.5% sodium alginate and 0.5% polyvinyl alcohol by mass percentage, and stand for defoaming; prepare calcium chloride with a mass percentage concentration of 0.5% and a mass percentage concentration at the same time A mixed aqueous solution of 0.05% doxorubicin;

[0022] b) Take the method of scraping film, pour the mixed aqueous solution of sodium alginate and polyvinyl alcohol prepared in step a) on a clean glass plate, scrape it with a scraping film rod with a mold thickness of 1000 μm, and then immediately use step a) The prepared calcium chloride aqueous solution was cross-linked and solidified for 12 hours, and rinsed with deionized water for 3-5 times to obtain a calcium alginate hydrogel film;

[0023]c) Prepare a diammonium hydrogen phosphate aqueous solution with a mass percentage of 0.05%, soak the ...

Embodiment 2

[0024] Example 2. A method for preparing a water-soluble vitamin slow-release carbonic acid / calcium alginate hybrid gel film

[0025] a) Prepare a mixed aqueous solution of 5% sodium alginate and 4% polyethylene glycol by mass percentage, and stand for defoaming; prepare calcium chloride and mass percentage concentration of 15% at the same time A mixed aqueous solution of 1% water-soluble vitamins;

[0026] b) Take the method of scraping film, pour the mixed aqueous solution of sodium alginate and polyethylene glycol prepared in step a) on a clean glass plate, scrape it with a scraping film rod with a mold thickness of 1000 μm, and then immediately use step a ) the prepared calcium chloride aqueous solution was cross-linked and solidified for 0.5 hours, rinsed with deionized water 3-5 times to obtain a calcium alginate hydrogel film;

[0027] c) preparing an aqueous solution of sodium carbonate with a mass percentage of 5%, soaking the calcium alginate hydrogel obtained in st...

Embodiment 3

[0028] Example 3. A preparation method of bovine serum albumin slow-release oxalic acid / calcium alginate hybrid gel microspheres

[0029] a) preparation mass percentage is the mixed aqueous solution of 1.5% sodium alginate and 0.5% carboxymethyl chitosan, stand-by after defoaming; preparation mass percentage concentration is 5% calcium chloride and A mixed aqueous solution of bovine serum albumin with a concentration of 10% by mass percentage;

[0030] b) The method of suspension dispersion is adopted, the mass percentage concentration of ethyl cellulose is 0.1%, the organic matter mixed with butyl acetate and castor oil according to the volume ratio of 1:3 is used as the continuous phase, and the alginic acid obtained in step a) is mixed under stirring The mixed aqueous solution of sodium and carboxymethyl chitosan is dispersed into droplet microspheres with a particle size of 0.5-500 μm, and then immediately poured into the aqueous calcium chloride solution prepared in step ...

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Abstract

The invention provides a preparation method of drug and protein sustained-release alginate hybrid gel. The preparation method comprises the steps of preparing calcium alginate hydrogel, then soaking the calcium alginate hydrogel in inorganic salt solutions including trisodium phosphate, diammonium hydrogen phosphate, sodium silicate, sodium carbonate, sodium oxalate and the like at certain concentration to swell for a certain period of time, and soaking the swelled calcium alginate hydrogel into an aqueous solution of drugs and proteins with calcium ions at certain concentration for crosslinking for a period of time, thus obtaining the drug and protein sustained-release alginate hybrid gel. The calcium alginate hydrogel disclosed by the invention can be in the shape of a membrane, a microballoon, a fiber and the like. The method disclosed by the invention is simple to operate and does not adopt any poisonous and harmful solvent; the activity of the immobilized protein is high; the materials are good in biocompatibility; and the drug and protein sustained-release alginate hybrid gel is good in slow release performance, and has good application prospect in the fields of tissue engineering, controlled release, medicine and health, and the like.

Description

technical field [0001] The invention relates to a preparation method of a drug and protein slow-release alginate hybrid gel, which belongs to the field of functional materials and biological materials. Background technique [0002] Sustained release of drugs is to combine small molecule drugs with polymer carriers by physical or chemical methods, and release small molecule drugs continuously at an appropriate concentration through control methods such as diffusion and penetration in the body, so as to achieve the purpose of fully exerting the efficacy of drugs . As the material of drug sustained-release carrier, it is an important part of the drug sustained-release system, an important substance to regulate the drug release rate, and the main factor affecting the efficacy of the drug. It needs to be biocompatible and biodegradable, that is, it can be degraded into small molecular compounds in the body, so that it can be metabolized, absorbed or excreted by the body, has no ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/34A61K47/38A61K47/32A61K45/00A61K38/00A61L27/26A61L27/20A61L27/52A61L27/54A61K47/10
Inventor 赵孔银林贝贝魏俊富李思迪任彤胡欣萍
Owner TIANJIN POLYTECHNIC UNIV
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