Method for quickly immobilizing proteins or amino-contained molecules on aminated surface

Abstract

The invention provides a method for quickly immobilizing proteins or amino-contained molecules on an aminated surface. Amino-contained molecules are firstly assembled on a solid-phase surface such asglass, silicon chip, gold and the like to form an aminated surface, a mixture of N-hydroxy-succinamide and 1-ethide-3-(3-dimethyllaminopropyl) carbodiimide is used for functionalizing the carboxyl ofa poly-carboxyl compound, the functionalized poly-carboxyl compound reacts with the aminated surface, the immobilization of the poly-carboxyl compound on the solid-phase surface is realized by the combination of the functionalized carboxyl and the amino, and the remaining functionalized carboxyl can react with protein molecules to be immobilized and other amino-containing molecules. The method canquickly introduce the functionalized carboxyl to the aminated surface, realize the immobilization of the proteins, simplify surface-modifying steps and shorten the time for immobilizing the proteins.The method has the advantages of simple operation and environment friendliness and is suitable for the covalent immobilization of the amino-contained molecules on the aminated surface.

Description

technical field [0001] The invention relates to a method for immobilizing protein or other amino-containing molecules on the surface of an amino-modified solid phase, and belongs to the technical field of surface chemistry and protein immobilization. Background technique [0002] At present, there are many methods for immobilizing proteins on the surface of solid chips, including physical adsorption, chemical covalent method, polymer embedding method, etc. Among them, the chemical covalent method to immobilize protein molecules has the characteristics of simple operation and strong bond formation. Using amino groups on the surface of protein molecules, through chemical reactions, protein molecules are covalently immobilized on the surface of modified chips: including aldehyde-modified surfaces, carboxylated-modified surfaces, and epoxy-silane-modified surfaces. [0003] Aldylation and carboxylation are both modified surfaces on amino-terminated silane molecules (such as amin...

AI Technical Summary

Problems solved by technology

Although the carboxyl-modified surface is relatively more stable than the aldehyde-modified surface, it can be stored more conveniently and for a longer period of time, but the pre-treatment of this method requires a long time (only the carboxylation modification step needs more than 3 hours), and Immobilizes proteins less efficiently than aldehyde-based surfaces

In addition, due to the use of succinic anhydride to treat the amino surface, the reaction process is slow, and the density of activated carboxyl groups on the surface of the chip cannot be controlled, and the immobilization efficiency of some protein molecules is related to the size of the carboxyl group density.

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