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Ionic liquid covalent-modified graphene-hydrotalcite-like composite membrane fixed protein-modified electrode, and preparation method and detection application thereof

A technology of ionic liquid and covalent modification, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., to achieve the effects of small Michaelis constant, suppressing sheet stacking, and enhancing conductivity

Inactive Publication Date: 2016-07-20
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of ionic liquid covalently modified graphene-exfoliated hydrotalcite-like composite (IL-GR-ELDH) by epoxy ring-opening reaction of GO-ELDH composite with amino-functionalized ionic liquid, and the preparation of protein immobilized by the complex IL-GR-ELDH-Protein Modified Electrode and Its Research on Trichloroacetic Acid (TCA) Detection Has Not Been Reported yet

Method used

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  • Ionic liquid covalent-modified graphene-hydrotalcite-like composite membrane fixed protein-modified electrode, and preparation method and detection application thereof
  • Ionic liquid covalent-modified graphene-hydrotalcite-like composite membrane fixed protein-modified electrode, and preparation method and detection application thereof
  • Ionic liquid covalent-modified graphene-hydrotalcite-like composite membrane fixed protein-modified electrode, and preparation method and detection application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (a) Preparation of GO-ELDH nano hybrid material

[0035] The GO was prepared by the hummer method, and a certain amount of the obtained GO was dispersed in formamide to a concentration of 1 mg / mL, ultrasonically dispersed for 1 hour, and centrifuged at 3000 rpm for 10 minutes to remove the unstripped GO to obtain a stripped GO dispersion; The GO dispersion and the ELDH formamide colloidal solution with a concentration of 1 mg / mL are fully mixed in equal volume, stirred for 12 hours under airtight conditions at room temperature, centrifuged at 5000 rpm for 10 minutes, and the brown-black precipitate obtained is the GO-ELDH hybrid;

[0036] (b) Preparation of IL-GR-ELDH hybrid material

[0037] Weigh 10 mg of the GO-ELDH hybrid obtained in step (a) and disperse it in deionized water to a concentration of 0.5 mg / mL. Slowly add 5 mg IL and 5 mg KOH and sonicate for 30 min; place the sonicated dispersion at 80°C Heat and stir for 24 hours, centrifuge at 4000 rpm for 5 minutes, and...

Embodiment 2

[0043] (a) Preparation of GO-ELDH nano hybrid material

[0044] Prepare according to the method and conditions in step (a) in Example 1;

[0045] (b) Preparation of IL-GR-ELDH hybrid material

[0046] Weigh 20mg of GO-ELDH hybrid and disperse it in deionized water to make the concentration 0.5mg / mL. Slowly add 10mgIL and 10mgKOH and sonicate for 30min; heat and stir the sonicated dispersion at 80℃ for 24h, and centrifuge at 4000rmp for 5min , Washed with water and absolute ethanol 3 times, the resulting black solid particles are IL-GR-ELDH hybrid;

[0047] (c) Preparation of CILE

[0048] Prepare according to the method and conditions in step (c) in Example 1;

[0049] (d) Preparation of IL-GR-ELDH composite material immobilized protein modified electrode

[0050] The IL-GR-ELDH hybrid material obtained in step (b) is formulated into a slurry with a concentration of 0.5 mg / mL with deionized water, and 10 μL of the slurry is applied dropwise to the CILE obtained in step (c) After natural...

Embodiment 3

[0052] (a) Preparation of GO-ELDH nano hybrid material

[0053] Prepare according to the method and conditions in step (a) in Example 1;

[0054] (b) Preparation of IL-GR-ELDH hybrid material

[0055] Weigh 30mg of GO-ELDH hybrid and disperse it in deionized water to make the concentration 0.5mg / mL. Slowly add 15mgIL and 15mgKOH and sonicate for 30min; heat and stir the sonicated dispersion at 80℃ for 24h, centrifuge at 4000rmp for 5min , Washed with water and absolute ethanol 3 times, the resulting black solid particles are IL-GR-ELDH hybrid;

[0056] (c) Preparation of CILE

[0057] Prepare according to the method and conditions in step (c) in Example 1;

[0058] (d) Preparation of IL-GR-ELDH composite material immobilized protein modified electrode

[0059] The IL-GR-ELDH hybrid material obtained in step (b) was prepared into a slurry with a concentration of 0.5 mg / mL with deionized water, and 9 μL of the slurry was dropped and applied to the CILE obtained in step (c) After natural d...

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Abstract

The invention discloses an ionic liquid covalent-modified graphene-stripped hydrotalcite-like composite material fixed protein-modified electrode, and a preparation method and an application thereof in detection of trichloroacetic acid. The special solubility and high conductivity of an ionic liquid are utilized, the ionic liquid is introduced into the surface of a graphene-stripped hydrotalcite-like composite material through a covalent modification method, an ionic liquid covalent-modified graphene-stripped hydrotalcite-like hybrid is prepared, and the ionic liquid covalent-modified graphene-stripped hydrotalcite-like composite material fixed protein-modified electrode is prepared by a dropwise coating method. The obtained modified electrode plays the synergistic effect of the ionic liquid, graphene and stripped hydrotalcite-like flakes in improvement of hemoglobin direct electrochemistry and electrocatalytic properties, and the conductivity, dispersivity and biocompatibility of the modified electrode are improved. A third-generation trichloroacetic acid sensor based on the biological modified electrode is built and has the advantages of low detection limit, wide detection range and small Michaelis constant and the like.

Description

Technical field: [0001] The invention relates to an amino-functionalized ionic liquid covalently modified graphene-exfoliated hydrotalcite sheet composite material immobilized protein modified electrode; the invention also relates to a preparation method of the modified electrode and its application in electrochemical sensing and detection . Background technique: [0002] A biosensor is a type of analysis device that combines the sensitive elements of biological or biological derivatives and physical and chemical transducers. It can generate intermittent or continuous signals according to the concentration of the analyte, and the signal strength is proportional to the concentration [Zhang Xianen, Biosensor [M], Beijing: Chemical Industry Press, 2006, 16-40.]. Among them, enzyme electrochemical sensors have attracted great attention in recent years. Bovine hemoglobin (Hb) has a simple and representative structure and is easy to achieve large-scale production, so it is used as an...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277G01N27/3278
Inventor 詹天荣王新军芦思思谭正伟
Owner QINGDAO UNIV OF SCI & TECH
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