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Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof

An ionic liquid and composite material technology is applied in the field of functionalized ionic liquid-hydrotalcite-like composite immobilized protein modified electrodes, and achieves the effects of wide detection range, high sensitivity and low preparation cost

Active Publication Date: 2015-03-11
佛山市顺德区德维医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the research on functionalized ionic liquid-inorganic nanocomposite modified electrodes is relatively active, most of them directly physically mix functionalized ionic liquids, inorganic nanomaterials and biological enzymes to prepare composite modified electrodes. At present, there is no co-precipitation method. Modified electrode prepared by immobilizing protein in functionalized ionic liquid-LDH composite material and its report on hydrogen peroxide detection

Method used

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  • Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof
  • Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof
  • Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Divalent metal salt Zn(NO 3 ) 2 and trivalent metal salt Al(NO 3 ) 3 Prepare a mixed salt solution at a certain molar ratio of 2:1, the total metal ion concentration is 0.6 mol / L, add Hb to the mixed salt solution at a mass ratio of Hb:LDH of 0.05, and then add IL OH According to its combination with divalent metal salt Zn(NO 3 ) 2 The molar ratio of 1:2 is added to the mixed salt solution, and the above mixed salt solution is titrated with the sodium hydroxide solution with a molar concentration of 2 mol / L at the same time, and the pH is controlled between 9.0 and 10.0. Aging for 18 hours; the obtained slurry was suction filtered, the filter cake was washed 5 times with distilled water, and dried at room temperature for 24 hours to obtain the composite material Hb-IL loaded with Hb OH -LDH; The obtained composite material Hb-IL loaded with hemoglobin OH -LDH was prepared as a modification solution with a Hb content of 20 μg / μL, and 7 μL was drip-coated with 1 mol...

Embodiment 2

[0038] Divalent metal salt Zn(NO 3 ) 2 and trivalent metal salt Al(NO 3 ) 3 Prepare a mixed salt solution at a certain molar ratio of 2:1, the total metal ion concentration is 0.6 mol / L, add Hb to the mixed salt solution at a mass ratio of Hb:LDH of 0.25, and then add IL OH According to its combination with divalent metal salt Zn(NO 3 ) 2 The molar ratio of 1:2 is added to the mixed salt solution, and the above mixed salt solution is titrated with the sodium hydroxide solution with a molar concentration of 2 mol / L at the same time, and the pH is controlled between 9.0 and 10.0. Aging for 20 hours; the obtained slurry was suction filtered, the filter cake was washed 5 times with distilled water, and dried at room temperature for 24 hours to obtain the composite material Hb-IL loaded with Hb OH -LDH; The obtained composite material Hb-IL loaded with hemoglobin OH -LDH was prepared as a modification solution with a Hb content of 20 μg / μL, and 7 μL was drip-coated with 1 mol...

Embodiment 3

[0040] Divalent metal salt Zn(NO 3 ) 2 and trivalent metal salt Al(NO 3 ) 3 Prepare a mixed salt solution at a certain molar ratio of 2:1, the total metal ion concentration is 0.6 mol / L, add Hb to the mixed salt solution at a mass ratio of Hb:LDH of 0.5, and then add IL OH According to its combination with divalent metal salt Zn(NO 3 ) 2 The molar ratio of 1:2 is added to the mixed salt solution, and the above mixed salt solution is titrated with the sodium hydroxide solution with a molar concentration of 2 mol / L at the same time, and the pH is controlled between 9.0 and 10.0. Aging for 22 hours; the resulting slurry was suction filtered, the filter cake was washed 5 times with distilled water, and dried at room temperature for 24 hours to obtain the composite material Hb-IL loaded with Hb OH -LDH; The obtained composite material Hb-IL loaded with hemoglobin OH -LDH was prepared as a modification solution with a Hb content of 20 μg / μL, and 7 μL was drip-coated with 1 mol...

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Abstract

The invention discloses a functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as a preparation method and an application thereof. According to the technical scheme provided by the invention, firstly the fixation of an ionic liquid-hydrotalcite-like composite material to hemoglobin is realized by utilizing a coprecipitation method, then obtained hemoglobin-functionalized ionic liquid-hydrotalcite-like composite material is prepared into a solution which is dropwise coated on the surface of a preprocessed glassy carbon electrode, and the functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode is obtained after natural drying. According to the obtained composite modified electrode, the superiorities of functionalized ionic liquid and layered inorganic materials in the aspect of electrode modified materials are adequately combined, so that the modified electrode has high electrical conductivity and good dispersibility and enzyme fixation capacity. A hydrogen peroxide electrochemical sensor based on the modified electrode has the advantages of simplicity in operation, mild condition, low detection limit, high sensitivity, wide linear range and the like.

Description

Technical field [0001] The present invention involves a functional ionic liquid-water talc composite material fixed protein modified electrode; the present invention also involves the preparation method of modified electrodes and its application in electrochemical sensing detection. Background technique [0002] Direct electronic chemistry research on oxidation and restore protein and enzymes can not only help people deeply understand the electronic transmission mechanism of biological systems, but also provide important theoretical foundation and technical support for the development of new biosensor, new biofuel batteries.However, their active center is usually buried deeply, and it is difficult to directly electrochemistry on the surface of the electrode, and they are prone to falling off on the surface of the nude electrode.Therefore, finding the activity that can maintain enzymes and promoting direct electron transmission materials have become an important topic in the field...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 詹天荣侯万国贾思佳徐洁
Owner 佛山市顺德区德维医疗科技有限公司
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