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Graphene-exfoliated hydrotalcite flake composite immobilized protein modified electrode and its preparation method and application

A composite material and protein modification technology, which is applied in the field of graphene-exfoliated hydrotalcite sheet composite immobilized protein modified electrodes, to achieve the effect of improving electron transfer rate, increasing conductivity and reducing contact resistance

Active Publication Date: 2017-11-07
徐州圣都金属科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, negatively charged GO is combined with positively charged ELDH by means of electrostatic attraction, and then the GR-ELDH complex is prepared by reduction, and the protein is fixed by the drop method to prepare the GR-ELDH-protein modified electrode and its application in the detection of hydrogen peroxide. Research on this aspect has not yet been reported

Method used

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  • Graphene-exfoliated hydrotalcite flake composite immobilized protein modified electrode and its preparation method and application
  • Graphene-exfoliated hydrotalcite flake composite immobilized protein modified electrode and its preparation method and application
  • Graphene-exfoliated hydrotalcite flake composite immobilized protein modified electrode and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0035] (a) Preparation of ELDH

[0036] divalent metal salt CoCl 2 ·6H 2 O, trivalent metal salt AlCl 3 ·6H 2 Add O and urea into a round bottom flask with a certain volume of deionized water at a molar ratio of 2:1:7, the total metal ion concentration is 0.15mol / L, stir and reflux at 85°C for 18 hours, and suction filter after the reaction is completed , the filter cake was washed with distilled water for 3 to 5 times, and dried at room temperature for 48 hours to obtain carbonate-intercalated LDH; weigh 100 mg of carbonate-intercalated LDH, and disperse it in NaCl and 3.3 mmol / L concentrations of 1 mol / L and 3.3 mmol / L respectively. mixed aqueous solution of HCl, N 2 Stir under the atmosphere for 12 hours, and dry at room temperature for 12 hours after suction filtration to obtain LDH intercalated with chloride ions; weigh 60 mg of LDH intercalated with chloride ions, and disperse it in 60 mL of NaNO with a concentration of 0.1 mol / L 3 in aqueous solution, N 2 Stir und...

Embodiment 2

[0044] (a) Preparation of ELDH

[0045] divalent metal salt CoCl 2 ·6H 2 O, trivalent metal salt AlCl 3 ·6H 2 O and urea were added into a round bottom flask with a certain volume of deionized water in a molar ratio of 2:1:7, the total metal ion concentration was 0.15mol / L, stirred and refluxed at 90°C for 24 hours, and pumped out after the reaction was completed. filter, the filter cake was washed with distilled water for 3 to 5 times, and dried at room temperature for 48 hours to obtain carbonate-intercalated LDH; weigh 100 mg of carbonate-intercalated LDH, and disperse it in NaCl and 3.3 mmol / L concentrations of 1 mol / L and 3.3 mmol / L respectively. L mixed aqueous solution of HCl, N 2 Stir under the atmosphere for 12 hours, and dry at room temperature for 12 hours after suction filtration to obtain LDH intercalated with chloride ions; weigh 60 mg of LDH intercalated with chloride ions, and disperse it in 60 mL of NaNO with a concentration of 0.1 mol / L 3 in aqueous solu...

Embodiment 3

[0053] (a) Preparation of ELDH

[0054] divalent metal salt CoCl 2 ·6H 2 O, trivalent metal salt AlCl 3 ·6H 2 O and urea were added into a round-bottomed flask with a certain volume of deionized water at a molar ratio of 2:1:7, the total metal ion concentration was 0.15mol / L, stirred and refluxed at 95°C for 32 hours, and pumped out after the reaction was completed. filter, the filter cake was washed with distilled water for 3 to 5 times, and dried at room temperature for 48 hours to obtain carbonate-intercalated LDH; weigh 100 mg of carbonate-intercalated LDH, and disperse it in NaCl and 3.3 mmol / L concentrations of 1 mol / L and 3.3 mmol / L respectively. L mixed aqueous solution of HCl, N 2 Stir under the atmosphere for 12 hours, and dry at room temperature for 12 hours after suction filtration to obtain LDH intercalated with chloride ions; weigh 60 mg of LDH intercalated with chloride ions, and disperse it in 60 mL of NaNO with a concentration of 0.1 mol / L 3 in aqueous so...

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Abstract

The invention discloses a graphene (GR)-exfoliated hydrotalcite sheet (ELDH) composite material immobilized protein modified electrode, a preparation method and application thereof. The present invention utilizes the characteristics of positive charge and large specific surface area of ​​ELDH, composites with negatively charged exfoliated graphene oxide nanosheets (GO) through electrostatic attraction, and then reduces with hydrazine hydrate to prepare GR‑ELDH hybrids. GR‑ELDH, hemoglobin and chitosan were sequentially immobilized on the ionic liquid modified carbon paste electrode by the drop coating method, and the graphene‑exfoliated hydrotalcite-like flake composite immobilized protein modified electrode was prepared. The modified electrode obtained in the present invention exerts the synergistic effect of single-layer GR nanosheets and ELDH. GR nanosheets increase the conductivity of ELDH and inhibit its aggregation and accumulation; ELDH effectively inhibits the re-stacking of GR sheets and reduces the number of sheets. The contact resistance between them improves the electron transfer rate of the composite material, and the third-generation trichloroacetic acid sensor based on the CTS / GR-ELDH-Hb composite film constructed has the advantages of low detection limit, wide detection range and small Michaelis constant. .

Description

Technical field: [0001] The invention relates to a graphene-exfoliated hydrotalcite sheet composite material immobilized protein modified electrode; the invention also relates to a preparation method of the modified electrode and its application in electrochemical sensing and detection. Background technique: [0002] A biosensor is a type of analytical device that combines sensitive elements of organisms or biological derivatives with physical and chemical transducers. It can generate intermittent or continuous signals according to the concentration of the analyte, and the signal strength is proportional to the concentration [Zhang Xianen, biosensor [M], Beijing: Chemical Industry Press, 2006, 16-40.]. Among them, enzyme electrochemical sensors have attracted great attention in recent years. Bovine hemoglobinase (Hb) is an ideal model for direct electrochemistry because of its simple and representative structure and its ease of large-scale production. At the same time, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/26
Inventor 詹天荣康敬霞王新军谭正伟
Owner 徐州圣都金属科技有限公司
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