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Method for immobilizing protein on TiO2 and regulating and controlling cell affinity by utilizing ultraviolet irradiation and TiO2-protein products

A technology of affinity and external irradiation, which is applied in the preparation method of peptides, chemical instruments and methods, albumin peptides, etc., can solve the problem of reducing the two-way regulation of protein cell affinity

Inactive Publication Date: 2018-04-20
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, so far, there are still few reports at home and abroad that can achieve two-way regulation of protein cell affinity by simply changing the treatment conditions.

Method used

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  • Method for immobilizing protein on TiO2 and regulating and controlling cell affinity by utilizing ultraviolet irradiation and TiO2-protein products
  • Method for immobilizing protein on TiO2 and regulating and controlling cell affinity by utilizing ultraviolet irradiation and TiO2-protein products
  • Method for immobilizing protein on TiO2 and regulating and controlling cell affinity by utilizing ultraviolet irradiation and TiO2-protein products

Examples

Experimental program
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Effect test

Embodiment 1

[0045] This embodiment provides a TiO 2 - bovine albumin composite surface, which is specifically prepared according to the following method:

[0046] TiO 2 The material was placed in bovine albumin solution, so that TiO 2 Adsorb bovine albumin on the surface, take it out and wash it with RO water, wash off the unadsorbed bovine albumin, and get TiO 2 - bovine albumin sample.

[0047] wet TiO 2 -Wet full UV irradiation of bovine albumin samples in water to obtain TiO with the ability to resist cell adhesion 2 - Bovine albumin composite surface.

Embodiment 2

[0049] This embodiment provides a TiO 2 - bovine albumin composite surface, which is specifically prepared according to the following method:

[0050] TiO 2 The material was placed in bovine albumin solution, so that TiO 2 Adsorb bovine albumin on the surface, take out the bovine white and wash it with RO water to wash off the unadsorbed bovine albumin to obtain TiO 2 - bovine albumin sample.

[0051] TiO 2 - Bovine albumin samples were air dried naturally.

[0052] The dried TiO 2 - Bovine albumin samples were subjected to dry full-UV irradiation in a dry environment to obtain TiO with good cell affinity 2 - Bovine albumin composite surface.

Embodiment 3

[0054] This embodiment provides a TiO 2 - bovine albumin cell micropattern, which is specifically prepared according to the following method:

[0055] TiO 2 The material was placed in bovine albumin solution, so that TiO 2 Adsorb bovine albumin on the surface, take out the bovine white and wash it with RO water to wash off the unadsorbed bovine albumin to obtain TiO 2 - bovine albumin sample.

[0056] wet TiO 2 - Wet-masked UV-irradiation of bovine albumin samples in water to obtain TiO with the ability to resist cell adhesion 2 - protein cell micropatterned surface.

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Abstract

The invention discloses a method for immobilizing a protein on TiO2 and regulating and controlling cell affinity by utilizing ultraviolet irradiation and TiO2-protein products, and relates to the field of biological materials. According to embodiments provided by the invention, the method for immobilizing the protein on the TiO2 and regulating and controlling the cell affinity by utilizing the ultraviolet irradiation comprises the steps of placing a TiO2 material into a protein solution to make the protein adsorbed at the TiO2 surface, taking out the treated material, and performing washing with water to wash off an unadsorbed protein, so as to obtain a TiO2-protein sample; and performing dry or wet ultraviolet irradiation on the TiO2-protein sample to obtain the TiO2-protein products withdifferent cells affinity. The method for immobilizing the protein on the TiO2 and regulating and controlling the cell affinity by utilizing the ultraviolet irradiation provided by the invention can effectively regulate and control the cell affinity of the protein adsorbed at the TiO2 surface; and the obtained TiO2-protein products can meet the personalized needs of different biological material application fields on the biological properties of materials, and have important practical value.

Description

technical field [0001] The present invention relates to the field of biomaterials, and in particular to a method of using ultraviolet radiation on TiO 2 A method for immobilizing proteins and regulating cell affinity and TiO 2 - protein products. Background technique [0002] Protein is the basic constituent material of organisms, and it participates in every process of cell life activities. Immobilizing proteins on the surface of materials can provide materials with unique biocompatibility and various biological functions, which can be applied in many fields such as bioelectronics, tissue engineering, and drug release. [0003] At present, there are two main methods for immobilizing proteins on the surface of materials: (1) self-assembly method: using a small number of proteins with self-assembly properties, such as silk fibroin, to self-assemble and fix on the surface of materials; (2) cross-linking agent method : Use a cross-linking agent to immobilize the protein on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/04C07K14/75C07K14/76
CPCC07K1/04C07K1/042C07K14/75C07K14/76
Inventor 杨苹陈江吕先锋廖玉珍向攀黄楠
Owner SOUTHWEST JIAOTONG UNIV
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