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Mesoporous bioactive glass/poly-decyl diacid glyceride composite support as well as preparation method and application thereof

A technology of polyglycerol sebacate and polyglycerol sebacate, which is applied in the fields of material science and medicine, can solve the problems of limited application, promote cell adhesion and proliferation, improve bone repair ability and new bone growth ability, Achieving the effect of complete regeneration/repair

Active Publication Date: 2015-05-27
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, its application mainly focuses on the utilization and research of the high elasticity of cross-linked PGS materials. However, the high temperature conditions involved in the conventional preparation process of cross-linked PGS limit its application in pre-immobilized protein scaffold systems.
For the adhesive performance, biocompatibility and biodegradability of non-cross-linked PGS, there is no application and research involved at present, and the application of non-cross-linked PGS itself or the combination of MBG and non-cross-linked PGS in the field of bone tissue engineering is still an issue. Blank

Method used

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  • Mesoporous bioactive glass/poly-decyl diacid glyceride composite support as well as preparation method and application thereof
  • Mesoporous bioactive glass/poly-decyl diacid glyceride composite support as well as preparation method and application thereof
  • Mesoporous bioactive glass/poly-decyl diacid glyceride composite support as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] The preparation method of composite support comprises the following steps:

[0054] (a) Coating PGS on the MBG porous scaffold;

[0055] (b) The scaffold is freeze-dried to obtain a composite scaffold.

[0056] Preferably, the mesoporous bioglass scaffold matrix is ​​prepared by a sol-gel method combined with a PU sponge template method. In a preferred embodiment, after uniformly mixing 100 parts by weight of MBG sol and 1 to 10 parts by weight of MBG powder, the mixture is mixed at 0.4 to 0.9 g / cm 3 The mass-to-volume ratio is poured on PU sponge, aged, dried, and calcined to obtain MBG porous scaffold, that is, the mesoporous bioglass scaffold matrix. In a preferred embodiment, with TEOS (orthoethyl silicate), Ca (NO 3 )·4H 2 O. TEP (triethyl phosphate) is the raw material, and F127 is the mesoporous template agent. Add 1-15 parts by weight of the mesoporous template agent into ethanol, adjust the pH to less than 2 with hydrochloric acid, and add four 0.3-5 parts...

Embodiment 1

[0086] Preparation of MBG sols and powders

[0087] 8g polyoxyethylene / polyoxypropylene / polyoxyethylene amphiphilic block copolymer ( F127, referred to as F127) and 6ml 1M HCl were added to 100g ethanol, and after complete dissolution, 1.52g Ca(NO 3 )·4H 2 O (calcium nitrate tetrahydrate) and 0.43ml TEP (triethyl phosphate), after completely dissolved, 10.4g TEOS (tetraethyl orthosilicate) was added dropwise, stirred vigorously at 40°C for 24h, and the obtained solution was concentrated by evaporation at 60°C to The viscosity is 5×10 4 MPa to get MBG sol.

[0088] Dry the prepared sol (60°C, 72 hours), grind it into powder with a mortar, and perform high-temperature calcination according to the following heating program: 20°C~600°C (580min, 1°C / min), 600°C~600°C (360min), end. Obtained MBG powder, wide-angle XRD pattern (A) and small-angle XRD pattern (B), nitrogen adsorption-desorption curve (C) and mesopore size distribution pattern (D) are as figure 1 As shown, the t...

Embodiment 2

[0090] Preparation of MBG scaffold with 60% porosity (60%MBG)

[0091] The MBG sol and MBG powder prepared in Example 1 were uniformly mixed in a ratio of 6g / 0.1g, and mixed at a rate of 0.857g / cm 3 The mass-to-volume ratio is poured on the pre-cut polyurethane PU sponge, aged at room temperature for 24 hours, dried at 60°C for 72 hours, and high-temperature calcined according to the following heating program: 20°C~600°C (580min, 1°C / min), 600°C ℃~600℃(360min), end. MBG scaffolds with a porosity of 60% are available (labeled 60% MBG).

[0092] The overall morphology of the obtained 60% MBG scaffold is as follows figure 2 As shown in A, the scanning electron microscope (SEM) photos taken by the macroporous structure are as follows: figure 2 As shown in the first column of B, the observed outline of the macropore structure in the outer layer is marked with a green line, and the measured average macropore diameter is 113.1 μm.

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Abstract

The invention discloses a mesoporous bioactive glass / poly-decyl diacid glyceride composite support as well as a preparation method and an application thereof. The composite support of the invention uses a mesoporous bioactive glass porous support as a matrix and compounds poly-decyl diacid glyceride on the mesoporous bioactive glass porous support. Mechanical strength, degradation rate, drug release rate, cell behavior and the like of the composite support of the invention can be adjusted by pore wall thickness of the mesoporous bioactive glass matrix and thickness of the poly-decyl diacid glyceride coating. A PGS coating is utilized to successfully solve the problem of brittleness of an MBG support, so that mechanical strength of the composite support is adjustable in a load bearing range of a trabecular bone. According to the invention, adjustment to degradation rate and release rate of proteins immobilized in MBG mesopore can be realized and activities of the immobilized proteins free of influence from a moderate coating operation can be realized; and cell experiments prove that the PGS coating is capable of promoting cell adhesion and proliferation on surface of the support without affecting osteogenic activity of the MBG.

Description

technical field [0001] The present invention relates to the fields of material science and medicine, in particular to a composite scaffold of medical bone repair materials, in particular to an MBG (mesoporous bioglass) / PGS (polyglyceryl sebacate) composite scaffold system and its preparation method and application. Composite scaffold materials can be used as bone tissue engineering scaffold materials and drug slow-release carriers. Background technique [0002] Diseases, trauma, population aging and natural disasters can all lead to damage to human bone tissue, and there is a huge demand for bone repair materials in clinical practice. As the golden rule, autologous bone transplantation is far from being able to meet the status quo due to its limited source and the pain of secondary surgery for patients. Allogeneic bone transplantation also has biological safety hazards, which may leave lifelong immune rejection and bring harm to patients. to unknown disease. Therefore, art...

Claims

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Application Information

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IPC IPC(8): A61L27/42A61L27/54A61K47/34A61K47/04
Inventor 刘昌胜林丹杨凯袁媛
Owner EAST CHINA UNIV OF SCI & TECH
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