A chip for cell culture, including a laminate having a flow path structure inside, in which the laminate includes a bottom plate substrate, a flow path substrate in which a flow path is formed by laserprocessing, and a top plate substrate in this order, and the flow path substrate includes a resin having a glass transition temperature of 37°C or higher, an elastic modulus at 25°C of 1 × 109 Pa or more, and an elastic modulus at 150°C of 1 × 107 Pa or less.