The utility model provides a microfluidic
cell separation and detection device, and belongs to the field of
cell detection. The device comprises a case, wherein a separation unit, a heating unit, a
control system and a
fluorescence detection module are arranged on the case; the heating unit and the
fluorescence detection module are connected with the separation unit and the
control system; the separation unit comprises a
chip fixing frame and a micro-fluidic
chip embedded in the
chip fixing frame; the micro-fluidic chip is provided with a liquid
inlet channel, an annular combination
pool and a liquid outlet channel, the liquid
inlet channel is provided with a liquid inlet, the liquid
inlet channel is correspondingly communicated with the liquid inlet side of the annular combination
pool, the liquid outlet channel is provided with a waste liquid
discharge hole, and the liquid outlet channel is correspondingly communicated with the liquid outlet side of the annular combination
pool; the heating module is arranged between the chip fixing frame and the annular combination pool; the annular combination pool is provided with a plurality of separation reaction cavities and
mineral oil adding holes which are communicated with one another. According to the utility model, various
cell types can be efficiently and specifically separated and detected, meanwhile, the operation process is simplified, and the analysis efficiency and accuracy are improved.