Primers capable of early diagnosis of cryptococcus infections and application thereof
A technology for early diagnosis and cryptococcal infection, applied in the direction of microorganisms, microbiological-based methods, biochemical equipment and methods, etc., can solve the problems of high technical and laboratory platform conditions, long time period for diagnosis of cryptococcal infection, etc., and achieve operational Strong compatibility, solve the effect of long diagnosis time period and convenient result interpretation
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[0030] Example 1 Screening of primers for early diagnosis of cryptococcal infection and verification of its specificity and sensitivity
[0031] 1. Main experimental materials and equipment
[0032] 1 strain
[0033] The standard strains CBS10083 (WM161), CBS10079 (WM629), CBS10078 (WM179), CBS10085 (WM148), CBS10080 (WM628), CBS10082 (WM178), CBS10083 ( WM626), CBS10101 (WM779) (purchased from CBS International Fungal Diversity Research Center in the Netherlands), H99, JEC20, and 41 standard strains and 34 clinical strains of Candida used to verify the specificity of the LAMP method were obtained from Shanghai Medical The strain bank of the Key Laboratory of Fungal Molecular Biology, with a total of 75 strains.
[0034] 2 devices
[0035] Ultra-clean bench: CA-1390-1 vertical laminar flow clean bench, Shanghai Shangjing Purification Equipment Co., Ltd.; biological safety cabinet: NUAIRE Biological Safefy Cabinet Class Ⅱ; water-proof electric heating constant temperature ...
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