Primers capable of early diagnosis of cryptococcus infections and application thereof

A technology for early diagnosis and cryptococcal infection, applied in the direction of microorganisms, microbiological-based methods, biochemical equipment and methods, etc., can solve the problems of high technical and laboratory platform conditions, long time period for diagnosis of cryptococcal infection, etc., and achieve operational Strong compatibility, solve the effect of long diagnosis time period and convenient result interpretation

Active Publication Date: 2013-09-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, based on DNA sequence analysis, try to find a rapid, simple, operable, and easy-to-interpret method for the rapid and early diagnosis of cryptococcal infection, which will help solve the problem of long diagnosis period of cryptococcal infection in clinical practice. It is of great significance to issues such as high requirements for testing personnel's technology and laboratory platform conditions, and will provide convenience for early diagnosis and timely treatment in clinical practice

Method used

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  • Primers capable of early diagnosis of cryptococcus infections and application thereof
  • Primers capable of early diagnosis of cryptococcus infections and application thereof
  • Primers capable of early diagnosis of cryptococcus infections and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Screening of primers for early diagnosis of cryptococcal infection and verification of its specificity and sensitivity

[0031] 1. Main experimental materials and equipment

[0032] 1 strain

[0033] The standard strains CBS10083 (WM161), CBS10079 (WM629), CBS10078 (WM179), CBS10085 (WM148), CBS10080 (WM628), CBS10082 (WM178), CBS10083 ( WM626), CBS10101 (WM779) (purchased from CBS International Fungal Diversity Research Center in the Netherlands), H99, JEC20, and 41 standard strains and 34 clinical strains of Candida used to verify the specificity of the LAMP method were obtained from Shanghai Medical The strain bank of the Key Laboratory of Fungal Molecular Biology, with a total of 75 strains.

[0034] 2 devices

[0035] Ultra-clean bench: CA-1390-1 vertical laminar flow clean bench, Shanghai Shangjing Purification Equipment Co., Ltd.; biological safety cabinet: NUAIRE Biological Safefy Cabinet Class Ⅱ; water-proof electric heating constant temperature ...

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Abstract

The invention relates to primers capable of early diagnosis of cryptococcus infections and application thereof. The primers are designed by using an IGS1 sequence of the most common clinical strain of newborn cryptococcus in the world-H99 strain as a target sequence by utilizing LAMP (loop-mediated isothermal amplification) professional primer design software. The experiment on effectiveness, specificity and sensitivity proves that the primers can be used for early diagnosis of cryptococcus infections, have the advantages of high speed, simpleness, strong operability and convenient result identification, solve the problems of long time period for diagnosing cryptococcus infections, low requirements for detection personnel technique and laboratory platform conditions and the like, can be made into a kit, and provide convenience for early diagnosis and timely treatment of cryptococcus infections.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a primer for early diagnosis of cryptococcal infection and its application. Background technique [0002] Invasive fungal diseases (IFDs) refer to systemic infectious diseases caused by pathogenic fungi invading heart, liver, spleen, lung, kidney, brain, blood and other internal organs. In the past two decades, due to the widespread use of immunosuppressants, various intubation techniques and the continuous spread of HIV, the morbidity and mortality of IFDs have continued to rise globally. The data from the China Hospital Infection Surveillance Network shows that IFDs It has become the second important component of nosocomial infection in my country (accounting for about 24.1%), among which Candida albicans ( Candida albicans ), Aspergillus fumigatus ( Aspergillus fumigatus ), Cryptococcus neoformans ( Cryptococcus neoformans ) are the top three pathogenic fungi o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/645
Inventor 陈敏李梦潘炜华廖万清
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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