Visual micro-fluidic chip for multi-virus nucleic acid detection, and detection method of visual micro-fluidic chip

A technology of microfluidic chips and viral nucleic acids, which is applied in chemical instruments and methods, biochemical equipment and methods, and microbial measurement/inspection, and can solve the problems of inability to efficiently, accurately, simply and quickly detect nucleic acids

Pending Publication Date: 2021-09-10
HARBIN INST OF TECH +1
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Problems solved by technology

[0004] The present invention provides a multi-virus nucleic acid detection visual microfluidic chip and its detection method, whic

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  • Visual micro-fluidic chip for multi-virus nucleic acid detection, and detection method of visual micro-fluidic chip
  • Visual micro-fluidic chip for multi-virus nucleic acid detection, and detection method of visual micro-fluidic chip
  • Visual micro-fluidic chip for multi-virus nucleic acid detection, and detection method of visual micro-fluidic chip

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Embodiment Construction

[0041] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0042] A multi-virus nucleic acid detection visual microfluidic chip, the visual microfluidic chip includes an extraction layer 10, a separation layer 11 and an amplification detection layer 12, and the extraction layer 10, separation layer 11 and amplification detection layer 12 Arranged from top to bottom;

[0043] In the extraction layer 10, the sample solution is divided into multiple reaction chambers;

[0044] The separation layer 11 separat...

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Abstract

The invention discloses a visual micro-fluidic chip for multi-virus nucleic acid detection, and a detection method of the visual micro-fluidic chip. An extraction layer, a separation layer and an amplification detection layer are arranged from top to bottom in sequence; the extraction layer is used for distributing a sample solution into a plurality of reaction cavities; the separation layer is used for separating the extraction layer from the amplification detection layer; and the amplification detection layer is used for carrying out amplification detection on different viral nucleic acids. The method is used for solving the problem that nucleic acid cannot be efficiently, accurately, simply and rapidly detected by aiming at POCT requirements.

Description

technical field [0001] The invention belongs to the field of microfluidic chips, and in particular relates to a visual microfluidic chip for multi-virus nucleic acid detection and a detection method thereof. Background technique [0002] At present, nucleic acid detection has been widely used in the field of clinical medicine, and its steps are mainly divided into: nucleic acid extraction, nucleic acid amplification, and nucleic acid detection. Traditional nucleic acid testing takes a lot of time and energy, and also relies on a strict and clean environment and professional equipment, which limits the development of point-of-care testing (POCT). The emergence of microfluidic technology provides a solution to this problem: the main form of microfluidic technology is a microfluidic chip, which integrates the functions of the entire analytical laboratory, including sampling, dilution, reagent addition, reaction, separation, and detection. and other technologies integrated on a...

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Application Information

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IPC IPC(8): C12M1/34C12M1/00C12Q1/70C12Q1/6844B01L3/00
CPCC12Q1/701C12Q1/6844B01L3/5027B01L2200/10C12Q2565/629C12Q2531/119
Inventor 李彪马晟利李雯曦聂桓张馨予陈以铖李禹克葛文玉李慧
Owner HARBIN INST OF TECH
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