Multi-target microarray chip full-automatic hybrid cleaning dyeing system

Abstract

The invention discloses a multi-target microarray chip full-automatic hybrid cleaning dyeing system comprising a multi-target microarray chip card box and a full-automatic hybrid cleaning dyeing system. The automatic hybrid cleaning dyeing system comprises a sample and reagent processing module, an incubation reaction module and a fluid control module. The disposable visual multi-target microarray chip card box adopts a fixed closed system, so that the risk of pollution is reduced, results are convenient to read, and fluid operation is convenient in a process that an instrument automatically performs a reaction; and the full-automatic hybrid cleaning dyeing system accelerates the experimental speed, and improves repeatability and consistency of experimental results.

Description

technical field [0001] The invention relates to a biological chip hybridization cleaning system, in particular to a fully automatic hybridization cleaning and dyeing system for multi-target microarray chips. Background technique [0002] The traditional microarray biochip analysis system generally includes several steps such as chip hybridization, cleaning, staining, and detection. At present, in most systems, in the chip hybridization, cleaning and staining process, the common treatment method is to place a fence on the surface of the glass chip to form a closed hybridization environment, which brings a lot of operational difficulties to sample addition and subsequent liquid treatment. Easy to automate. Since the above links are manually completed by the experimenters, it is not only time-consuming and labor-intensive, but also manual operations will bring experimental errors and the possibility of contamination. In addition, due to the limitation of the space of the hybr...

AI Technical Summary

Problems solved by technology

At present, in most systems, in the chip hybridization, cleaning and staining process, the common treatment method is to place a fence on the surface of the glass chip to form a closed hybridization environment, which brings a lot of operational difficulties to sample addition and subsequent liquid treatment. Easy to automate

Since the above links are manually completed by the experimenters, it is not only time-consuming and labor-intensive, but also manual operations will bring experimental errors and the possibility of contamination

In addition, due to the limitation of the space of the hybridization system in this hybridization method, it is difficult to form a homogeneous reaction, resulting in inhomogeneous hybridization of the microarray on the chip plane.

In addition, there are many fluid processing and biological reaction processes such as cleaning, labeling, and staining between the hybridization of the chip and the interpretation of the final result, which brings many difficulties to the stability of the experimental operation and results.

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