Breeding method of polyclonal antibody cold-resistant two-line sterile line

Abstract

The invention discloses a breeding method of a polyclonal antibody cold-resistant two-line sterile line, belonging to the technical field of breeding for disease resistance. According to the breeding method, by using a two-line sterile line as a recurrent parent, and using a tryporyza incertulas-resistant variety, a rice blast-resistant variety, a stunt-resistant variety and a cold-resistant variety as antigen parents, through a hybrid polymerization process, antigen genes of four rice diseases, insect and cold damages are polymerized in the same rice material, and then subjected to multi-generation backcross and screening together with the recurrent parent of the two-line sterile line, so that the insect and cold-resistant two-line sterile line, with economical characters, sterility period translative temperature and sterility highly consistent with those of the recurrent parent of the two-line sterile line, is bred. The two-line sterile line is used for preparing two-line hybrid rice, and thus the level of resisting insects and low-temperature coldness of the hybrid rice can be improved.

Description

technical field

[0001] The invention specifically relates to a method for breeding a multi-resistant and cold-tolerant two-line sterile line, and belongs to the technical field of rice resistance breeding. Background technique

[0002] As China's largest food crop, rice has made great contributions to solving China's food security and improving people's health. In recent years, rice pests and diseases have occurred seriously in my country, with rapid development of resistance and great pressure on prevention and control. There have been large-scale production reductions due to pests and diseases. Although spraying pesticides for prevention and control can achieve certain results, excessive spraying of pesticides not only causes serious grain damage. Pollution and environmental pollution have also increased the burden on farmers and raised food prices. Scientific research has proved that the most efficient, economical and environmentally friendly way to prevent and control ri...