37results about How to "Great potential for economic benefits" patented technology

The invention discloses a conductive polymer film and the preparation method thereof. The preparation method comprises the following steps: (1) dissolving polymer monomers in an organic solvent to obtain a solution A; (2) dissolving an oxidizer in water to obtain a solution B; and (3) mixing the solution A and the solution B to obtain a mixture C, and freezing the mixture C to form a crystal interface on which the monomers are polymerized to form the final product of conductive polymer film. The method has the advantages that the preparation technique is simple and convenient, the method has good controllability and wide application range and system, can be adapted to large-scale production, and is low in cost and great in economic benefit potential, and the conductive polymer film product is superior in electrical property.

The invention provides a corn bHLH transcription factor (Manganese-Induced Transcription Factor1) coding gene ZmMIT1 which expresses under induction of heavy metal manganese stress and use thereof for coding a protein. The gene can improve the resistance to heavy metal manganese stress in plants, in particular the manganese poisoning resistance in tobacco. The ZmMIT1 has a bright application prospect in field of heavy metal pollution resistance in corn.

The invention relates to an application of a corn ZmbZIP107 gene in cultivation of lead stress-resistant plants. Through qRT-PCR detection, the ZmbZIP107 gene enables inducible expression under lead stressed, and expression level of tolerant inbred line root is obviously higher than the expression level of sensitive inbred line roots. According to the invention, an arabidopis thaliana homologous gene mutant is used for researching, under lead stressed, the growth vigor of Columbia wild type is obviously better than that of a mutant, and the inhibition degree of the wild type is lower than that of the mutant. The ZmbZIP107 gene is located in a QTL section on No.10 chromosome, the gene enables inducible expression after the plant is subjected to lead stressing, and positive regulation and control can be carried out on responding lead stress by the plant, so that the lead stress-resistant capability of the plant is increased. The invention firstly clears the relation between the corn ZmbZIP107 gene and the salt stress-resistance of the plant, and the importance of the gene in the corn lead-resistant application is verified. The application provides theoretical basis and utilization value of the gene on corn and other plants to increase the stress resistance.

The invention discloses a preparation method for an iron-boron compound coating. An electrospark deposition process is adopted for preparation, and a used electrode is of a round-rod-shaped partially-hollow structure. An electrode material is a single phase Fe2B material, or a material composed of Fe2B and Fe3B, or a material composed of FeB and Fe2B. A prepared iron-boron compound in the coating obtained after electrospark deposition is conducted is Fe2B and Fe3B, or FeB and Fe2B or FeB, Fe2B and Fe3B, and furthermore, the coating contains a martensite phase with the weight fraction being smaller than 8%. Compared with a boriding process, the preparation method is easy to implement and environmentally friendly, and the prepared coating has the beneficial effects of being high in hardness, toughness, better in wear resistance and the like.

The invention provides application of arabidopsis U1A gene to plant salt tolerance improvement. The nucleotide sequence of the arabidopsis salt tolerance gene U1A is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein is shown as SEQ ID NO.2. The condition that the arabidopsis U1A gene participates in the plant salt stress resistant biological process is proved for the firsttime; the arabidopsis U1A gene is applied to salt tolerance plant culture; good application prospects are realized in the technical field of salt tolerance breeding.

The invention relates to a method for increasing the leaf number and the biomass of tobacco by utilizing a tobacco cytochrome C gene NtCYP94B3s. The nucleotide sequence of the tobacco cytochrome C gene NtCYP94B3s is shown as SEQ ID NO:1 in a sequence table. The method comprises the following steps of: firstly, constructing a plant RNAi vector, then, identifying agrobacterium tumefaciens-mediated tobacco transformation and transgenic plants, then, extracting the total RNA of wild plants and 25 pHellsgate12-transformed RNA gene T0-generation plants, performing Real time-PCR analysis with reference genes of 26s, analyzing expression conditions of different strains, and selecting two plants with the lowest expression quantity. According to the method in the invention, inhibition of the expression of the tobacco endogenous gene NtCYP94B3s in tobacco plants can obviously improve the leaf number and the biomass of the tobacco. The method has a wide application prospect and huge economic benefit potential.

The invention discloses a tobacco arsenic transportation gene NtNIP7-1, and a cloning method and an application of the gene NtNIP7-1. A nucleotide sequence of the tobacco arsenic transportation gene NtNIP7-1 is shown as SEQ ID (sequence identifier): No. (number) 1, and an encoded amino acid sequence is shown as SEQ ID: No. 2. The invention further discloses the cloning method of the tobacco arsenic transportation gene NtNIP7-1. The cloning method particularly comprises the steps of: extracting tobacco RNA (ribonucleic acid) for reverse transcription to form first chain cDNA (complementary deoxyribonucleic acid), taking first chain cDNA obtained by the reverse transcription as a template, designing and synthesizing a specific primer according to an NtNIP7-1 gene sequence, performing PCR (polymerase chain reaction) amplification, recovering and purifying a PCR amplification product, and performing sequencing. An arsenic content of a tobacco leaf can be reduced obviously by inhibiting expression of the tobacco endogenous gene NtNIP7-1 in a tobacco plant, and the gene NtNIP7-1 has wide application prospects in the field of low arsenic content seed breeding of plants and a great economic benefit potential.

The invention discloses a high cadmium-tolerant tobacco modified by a saccharomyces cerevisiae S288C vesicle transport gene MRS6 and its application. The nucleotide sequence of the gene MRS6 is shownin the formula of SEQ ID NO: 1. The amino acid sequence of the encoded protein is shown in the formula of SEQ ID NO: 2. In the present invention, it is found by the green fluorescent protein trace observation that the MRS6-encoded protein is mainly expressed in the ductal cells of the rhizomes of the transgenic plants. Through the treatment with CdCl2, compared with the wild type tobacco, the cadmium content enriched in the rhizome of the transgenic tobacco is significantly increased, the cadmium content in the leaves is significantly reduced, and the plant grows more rapidly. It is proved that the overexpression of MRS6 can increase the tolerance of tobacco to heavy metal cadmium, enhance the enrichment ability of cadmium, reduce the content of cadmium in leaves, and increase the growth rate of tobacco. Therefore, the tobacco improved by the gene MRS6 has high cadmium-tolerant activity, has broad application prospects in natural environment improvement based on environmental cadmium enriching, and has the great economic benefit potential.

The invention discloses a tobacco drought response gene NtRHF1 and an application of encoding protein thereof. The nucleotide sequence of the tobacco drought response gene NtRHF1 is shown by SEQ ID NO: 1, and the amino acid sequence of the encoding proteins is shown by SEQ ID NO: 2. The adaptability of plants to drought stress can be increased through the over-expression of the gene, and the tobacco drought response gene NtRHF1 has a wide application prospect in the field of tobacco drought.

The invention discloses a tobacco chloride ion absorbing gene NtSLAC2 and its cloning method and application. The nucleotide sequence of the tobacco chloride ion absorbing gene NtSLAC2 is shown in SEQ ID: No.1, and the encoded amino acid sequence is shown in SEQ ID: No.2. shown. The invention also discloses a method for cloning tobacco chloride ion absorbing gene NtSLAC2. The specific steps include: extracting tobacco RNA, and reverse-transcribing to obtain the first-strand cDNA; Synthesize specific primers, perform PCR amplification, recover and purify the PCR amplification products, and sequence them. In the present invention, suppress tobacco endogenous gene in tobacco plant NtSLAC2 The expression of can significantly reduce the chloride ion content of tobacco leaves, and has broad application prospects in the field of plant breeding with low chloride ion content, and its economic benefit potential is huge.

The invention relates to an application of a corn ZmbZIP107 gene in cultivation of lead stress-resistant plants. Through qRT-PCR detection, the ZmbZIP107 gene enables inducible expression under lead stressed, and expression level of tolerant inbred line root is obviously higher than the expression level of sensitive inbred line roots. According to the invention, an arabidopis thaliana homologous gene mutant is used for researching, under lead stressed, the growth vigor of Columbia wild type is obviously better than that of a mutant, and the inhibition degree of the wild type is lower than that of the mutant. The ZmbZIP107 gene is located in a QTL section on No.10 chromosome, the gene enables inducible expression after the plant is subjected to lead stressing, and positive regulation and control can be carried out on responding lead stress by the plant, so that the lead stress-resistant capability of the plant is increased. The invention firstly clears the relation between the corn ZmbZIP107 gene and the salt stress-resistance of the plant, and the importance of the gene in the corn lead-resistant application is verified. The application provides theoretical basis and utilization value of the gene on corn and other plants to increase the stress resistance.

The invention provides application of arabidopsis U1A gene to plant salt tolerance improvement. The nucleotide sequence of the arabidopsis salt tolerance gene U1A is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein is shown as SEQ ID NO.2. The condition that the arabidopsis U1A gene participates in the plant salt stress resistant biological process is proved for the firsttime; the arabidopsis U1A gene is applied to salt tolerance plant culture; good application prospects are realized in the technical field of salt tolerance breeding.

The invention relates to a method and device for improving an antibacterial peptide yield of bacillus amyloliquefaciens by a magnetic field. The method comprises 1, inoculating a seed medium with activated bacillus amyloliquefaciens and carrying out seed culture to obtain a seed liquid, 2, transferring the seed liquid obtained by the step 1 into a fermentation medium, carrying out preliminary fermentation until bacterial cells are in a logarithmic phase, carrying out continuous or discontinuous magnetic field fermentation, and carrying out follow-up fermentation treatment to obtain the antibacterial peptide-containing broth. The invention also relates to a device for magnetic field fermentation treatment. The bacillus amyloliquefacien cells in a logarithmic phase are treated in a magnetic field so that a desired product antibacterial peptide yield and antibacterial peptide activity are substantially improved. The method has simple processes, can be operated easily and substantially reduces an antibacterial peptide production cost.

The present invention discloses the application of maize ZmBES1 / BZR1-5 gene in cultivating large-grained plants. This patent clarifies the relationship between maize ZmBES1 / BZR1-5 gene and plant grain development, and verifies that maize ZmBES1 / BZR1-5 gene can improve plant growth. The effect of kernel size on application. The maize ZmBES1 / BZR1-5 gene can be used to breed high-yield crops, and the maize ZmBES1 / BZR1-5 gene has broad application prospects in the field of plant grain development, especially in the field of high-yield plants, and its economic benefit potential is huge.

The invention relates to a nuclear radiation protective supply and a preparation method of the nuclear radiation protective supply, in particular to a lead shielding block on the end face of a nuclear fuel channel in a heavy water reactor nuclear power station and a preparation method of the lead shielding block. The lead shielding block comprises multiple layers of overlying lead foil, wherein the lead foil is provided with locating holes, the fuel channel is sleeved with the lead shielding block through the locating holes, and silicone rubber layers are arranged on the outer side edges of the multiple layers of overlying lead foil and the inner wall faces of the locating holes. The preparation method of the lead shielding block comprises the steps of preparing the multiple layers of overlying lead foil, wrapping the multiple layers of overlying lead foil with silicone rubber, and conducting compression molding to obtain the lead shielding block. The lead shielding block does not damage the fuel channel and is light in self weight and easy to prepare, install and use.

The invention discloses an improved cadmium-resistant tobacco derived from S288C membrane vesicle transport gene MRS6 of Saccharomyces cerevisiae and its application. The nucleotide sequence of the gene MRS6 is shown in SEQ ID NO: 1; the amino acid sequence of the encoded protein is shown in SEQ ID NO: 2. In the present invention, it is found that the encoded protein of MRS6 is mainly expressed in a large amount in the vessel cells of the rhizome of the transgenic plant through the tracer observation of the green fluorescent protein. In the present invention, through CdCl 2 Treatment: Compared with wild-type tobacco, the cadmium content in the rhizome tissue of the transgenic tobacco was significantly increased, the cadmium content in the leaves was significantly reduced, and the plant grew faster. It proved that the overexpression of MRS6 can improve the tolerance of tobacco to heavy metal cadmium, enhance the ability to enrich cadmium, reduce the content of cadmium in leaves, and increase the growth rate of tobacco. Therefore, the tobacco improved by gene MRS6 of the present invention has high cadmium-tolerant activity, has broad application prospects in enriching environmental cadmium to improve natural environment, and has great potential for economic benefits.

The invention discloses the application of the corn ZmAKINβγ1 gene in cultivating lead stress-tolerant corn. The invention determines the relationship between the corn ZmAKINβγ1 gene and the corn lead stress resistance, and constructs an overexpression vector of the corn ZmAKINβγ1 gene and uses Agrobacterium to transfect corn young Embryos can up-regulate the expression of maize ZmAKINβγ1 gene, thereby effectively improving the lead tolerance of maize. This is because the gene has broad application prospects in the field of plant resistance to heavy metal pollution, especially in the field of maize resistance to soil lead poisoning, and its economic benefit potential is huge.

The invention discloses the application of the gene Zm00001d040827 in increasing the yield of corn. The invention determines the relationship between the Zm00001d040827 gene and the yield of corn. The Zm00001d040827 gene can increase the chlorophyll content of leaves, increase the weight of 100 kernels of corn, and increase the yield of corn per ear, thereby increasing the yield of corn. Yield. The invention verifies the importance of the gene in improving the yield of corn, and lays a theoretical basis for the application of the gene in improving the yield of corn.

The invention discloses a corn gene ZmLIP2 and an application thereof. ZmLIP2 gene transformation is performed on corns by using an agrobacterium-mediated transgenic technology, and an RNAi interference transformation event of the corn ZmLIP2 gene is obtained. An analysis on an expression quantity of RNAi-interfered plants shows that the expression quantity is remarkably reduced compared with thatof receptor materials. The gene can negatively regulate and control lead absorption and transport of plants, a relationship between the corn ZmLIP2 gene and lead stress resistance of the plants is clarified for the first time, and importance of the gene in a lead resistance application of the corns is verified.

The invention relates to the technical field of plant cells, and specifically discloses a gene that can be overexpressed to increase the potassium content of tobacco, its encoded product and its application. The gene is the PE1 gene, and the step of using the PE1 gene to construct a tobacco strain with high potassium content Including tobacco first-strand cDNA synthesis, recombinant vector pWM101-35S-PE1 construction and positive plant screening, recombinant vector pWM101-35S-PE1 construction through Agrobacterium activation, transfer, infection, co-cultivation, selective differentiation culture, rooting culture and transplanting steps. The potassium content in the transgenic high-potassium tobacco strain obtained by the method of the invention is greatly increased, and has broad application prospects in cultivating high-potassium tobacco varieties.

The invention provides a method for forward and reverse scouring and dredging of the diversion canal of a hydropower station and the structure of the diversion canal. The method includes: step S10, determining the gradation of the silt in the diversion canal; step S20, The grading curve determines the representative particle size of the silt and the corresponding starting flow rate; step S30, according to the Manning formula, determines the starting flow required to remove the silt by water flow; step S40, according to the The starting flow and the discharge capacity curve of the diversion canal determine the target opening of the diversion canal gate; step S50, according to the target opening, first open the diversion gate at the head of the diversion canal for forward flushing, Then open the gate of the power generation diversion tunnel at the tail of the diversion canal to carry out reverse flushing. Based on the technical scheme of the present invention, dredging by hydraulic forward and reverse flushing is used to remove a large amount of silted sand at relatively low cost, reducing the power generation loss and dredging cost of hydropower stations caused by silted sediment.