37results about How to "Great potential for economic benefits" patented technology

The invention discloses a preparation method for an iron-boron compound coating. An electrospark deposition process is adopted for preparation, and a used electrode is of a round-rod-shaped partially-hollow structure. An electrode material is a single phase Fe2B material, or a material composed of Fe2B and Fe3B, or a material composed of FeB and Fe2B. A prepared iron-boron compound in the coating obtained after electrospark deposition is conducted is Fe2B and Fe3B, or FeB and Fe2B or FeB, Fe2B and Fe3B, and furthermore, the coating contains a martensite phase with the weight fraction being smaller than 8%. Compared with a boriding process, the preparation method is easy to implement and environmentally friendly, and the prepared coating has the beneficial effects of being high in hardness, toughness, better in wear resistance and the like.

The invention relates to a method for increasing the leaf number and the biomass of tobacco by utilizing a tobacco cytochrome C gene NtCYP94B3s. The nucleotide sequence of the tobacco cytochrome C gene NtCYP94B3s is shown as SEQ ID NO:1 in a sequence table. The method comprises the following steps of: firstly, constructing a plant RNAi vector, then, identifying agrobacterium tumefaciens-mediated tobacco transformation and transgenic plants, then, extracting the total RNA of wild plants and 25 pHellsgate12-transformed RNA gene T0-generation plants, performing Real time-PCR analysis with reference genes of 26s, analyzing expression conditions of different strains, and selecting two plants with the lowest expression quantity. According to the method in the invention, inhibition of the expression of the tobacco endogenous gene NtCYP94B3s in tobacco plants can obviously improve the leaf number and the biomass of the tobacco. The method has a wide application prospect and huge economic benefit potential.

The invention discloses a tobacco chloride ion absorbing gene NtSLAC2 and its cloning method and application. The nucleotide sequence of the tobacco chloride ion absorbing gene NtSLAC2 is shown in SEQ ID: No.1, and the encoded amino acid sequence is shown in SEQ ID: No.2. shown. The invention also discloses a method for cloning tobacco chloride ion absorbing gene NtSLAC2. The specific steps include: extracting tobacco RNA, and reverse-transcribing to obtain the first-strand cDNA; Synthesize specific primers, perform PCR amplification, recover and purify the PCR amplification products, and sequence them. In the present invention, suppress tobacco endogenous gene in tobacco plant NtSLAC2 The expression of can significantly reduce the chloride ion content of tobacco leaves, and has broad application prospects in the field of plant breeding with low chloride ion content, and its economic benefit potential is huge.

The invention provides application of arabidopsis U1A gene to plant salt tolerance improvement. The nucleotide sequence of the arabidopsis salt tolerance gene U1A is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein is shown as SEQ ID NO.2. The condition that the arabidopsis U1A gene participates in the plant salt stress resistant biological process is proved for the firsttime; the arabidopsis U1A gene is applied to salt tolerance plant culture; good application prospects are realized in the technical field of salt tolerance breeding.

The invention relates to a method and device for improving an antibacterial peptide yield of bacillus amyloliquefaciens by a magnetic field. The method comprises 1, inoculating a seed medium with activated bacillus amyloliquefaciens and carrying out seed culture to obtain a seed liquid, 2, transferring the seed liquid obtained by the step 1 into a fermentation medium, carrying out preliminary fermentation until bacterial cells are in a logarithmic phase, carrying out continuous or discontinuous magnetic field fermentation, and carrying out follow-up fermentation treatment to obtain the antibacterial peptide-containing broth. The invention also relates to a device for magnetic field fermentation treatment. The bacillus amyloliquefacien cells in a logarithmic phase are treated in a magnetic field so that a desired product antibacterial peptide yield and antibacterial peptide activity are substantially improved. The method has simple processes, can be operated easily and substantially reduces an antibacterial peptide production cost.

The present invention discloses the application of maize ZmBES1 / BZR1-5 gene in cultivating large-grained plants. This patent clarifies the relationship between maize ZmBES1 / BZR1-5 gene and plant grain development, and verifies that maize ZmBES1 / BZR1-5 gene can improve plant growth. The effect of kernel size on application. The maize ZmBES1 / BZR1-5 gene can be used to breed high-yield crops, and the maize ZmBES1 / BZR1-5 gene has broad application prospects in the field of plant grain development, especially in the field of high-yield plants, and its economic benefit potential is huge.

The invention discloses an improved cadmium-resistant tobacco derived from S288C membrane vesicle transport gene MRS6 of Saccharomyces cerevisiae and its application. The nucleotide sequence of the gene MRS6 is shown in SEQ ID NO: 1; the amino acid sequence of the encoded protein is shown in SEQ ID NO: 2. In the present invention, it is found that the encoded protein of MRS6 is mainly expressed in a large amount in the vessel cells of the rhizome of the transgenic plant through the tracer observation of the green fluorescent protein. In the present invention, through CdCl 2 Treatment: Compared with wild-type tobacco, the cadmium content in the rhizome tissue of the transgenic tobacco was significantly increased, the cadmium content in the leaves was significantly reduced, and the plant grew faster. It proved that the overexpression of MRS6 can improve the tolerance of tobacco to heavy metal cadmium, enhance the ability to enrich cadmium, reduce the content of cadmium in leaves, and increase the growth rate of tobacco. Therefore, the tobacco improved by gene MRS6 of the present invention has high cadmium-tolerant activity, has broad application prospects in enriching environmental cadmium to improve natural environment, and has great potential for economic benefits.

The invention discloses the application of the corn ZmAKINβγ1 gene in cultivating lead stress-tolerant corn. The invention determines the relationship between the corn ZmAKINβγ1 gene and the corn lead stress resistance, and constructs an overexpression vector of the corn ZmAKINβγ1 gene and uses Agrobacterium to transfect corn young Embryos can up-regulate the expression of maize ZmAKINβγ1 gene, thereby effectively improving the lead tolerance of maize. This is because the gene has broad application prospects in the field of plant resistance to heavy metal pollution, especially in the field of maize resistance to soil lead poisoning, and its economic benefit potential is huge.

The invention relates to the technical field of plant cells, and specifically discloses a gene that can be overexpressed to increase the potassium content of tobacco, its encoded product and its application. The gene is the PE1 gene, and the step of using the PE1 gene to construct a tobacco strain with high potassium content Including tobacco first-strand cDNA synthesis, recombinant vector pWM101-35S-PE1 construction and positive plant screening, recombinant vector pWM101-35S-PE1 construction through Agrobacterium activation, transfer, infection, co-cultivation, selective differentiation culture, rooting culture and transplanting steps. The potassium content in the transgenic high-potassium tobacco strain obtained by the method of the invention is greatly increased, and has broad application prospects in cultivating high-potassium tobacco varieties.