Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of maize zmbes1/bzr1-5 gene in breeding large grain plants

A gene and plant technology, applied in the fields of application, plant peptides, plant products, etc., to achieve the effect of huge economic benefit potential

Inactive Publication Date: 2021-03-30
SICHUAN AGRI UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above content is basically the effect on a single rice or corn, but can not produce beneficial effects on both corn and rice.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of maize zmbes1/bzr1-5 gene in breeding large grain plants
  • Application of maize zmbes1/bzr1-5 gene in breeding large grain plants
  • Application of maize zmbes1/bzr1-5 gene in breeding large grain plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 gene cloning and vector construction

[0026] The leaves of corn seedlings at the five-leaf stage were taken, quick-frozen and ground in liquid nitrogen, and total RNA was extracted using the total RNA extraction kit Trizol (TaKaRa) according to its instructions. cDNA was synthesized using the total RNA as a template using PrimeScriptTM RT regeant Kit (Takara).

[0027] Use NCBI website Primer-BLAST to design the amplification primers (as shown in Table 1) of ZmBES1 / BZR1-5 gene, according to the multiple cloning site of dicotyledonous plant expression vector pCAMBIA2300-35-eGFP, enter the Sal I enzyme digestion at its upstream primer site, the downstream primer is introduced into the Spe I site, and the specific sequence is shown in Table 1:

[0028] Table 1 Amplification primer sequence of ZmBES1 / BZR1-5 gene

[0029]

[0030] Samples were loaded according to the reaction system shown in Table 2 for amplification. The temperature cycle program is: 94°...

Embodiment 2

[0039] Embodiment 2 Arabidopsis transformation and analysis of grain traits

[0040] The 35S-ZmBES1 / BZR1-5-eGFP vector was transformed into Arabidopsis Col-0 by Agrobacterium-mediated tidbit dipping method.

[0041] 1. Transformation by inflorescence dip-dyeing method

[0042] (1) Take the Agrobacterium containing the recombinant plasmid, streak it on a YEP plate containing kanamycin (Kana) and rifampicin (Rif), and culture it at 28°C for 2-3 days.

[0043] (2) Pick a single colony of Agrobacterium and inoculate it in 3 mL of YEP liquid medium containing Kana and Rif, and cultivate overnight at 28°C with shaking at 200 r / min.

[0044] (3) In 100 mL of liquid YEP medium (containing Kana and Rif), inoculate 1 mL of the initial Agrobacterium liquid cultured overnight, and culture with shaking at 28°C until the OD600 value of the bacterial liquid is 1.2-1.5.

[0045] (4) Collect the cells by centrifuging at 5000 r / min at 4°C for 10 min, suspend the cells with 5% sucrose solution...

Embodiment 3

[0069] Example 3 Rice Transformation and Analysis of Grain Characters

[0070] To further confirm the function of ZmBES1 / BZR1-5, we transformed 35S-ZmBES1 / BZR1-5-eGFP into rice (Zhonghua 11) for overexpression.

[0071] 1. Agrobacterium-mediated transformation of rice

[0072] (1) Rice callus induction and subculture

[0073] Pick mature rice seeds and peel off the chaff, pour them into a 50ml centrifuge tube, add 75% ethanol to disinfect for 1 minute, pour off the ethanol, rinse with sterile water, add 30% sodium hypochlorite to disinfect for 20 minutes, rinse with sterile water 5-6 times . Blot dry with sterilized filter paper and transfer seeds to induction medium with 20-25 seeds per dish. The callus was used for Agrobacterium transformation after outgrowth.

[0074] (2) Agrobacterium culture and infection

[0075] Agrobacterium EHA105 containing the 35S-ZmBES1 / BZR1-5-eGFP vector was streaked on a plate containing kanamycin and rifampicin, and cultured in the dark at ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention discloses the application of maize ZmBES1 / BZR1-5 gene in cultivating large-grained plants. This patent clarifies the relationship between maize ZmBES1 / BZR1-5 gene and plant grain development, and verifies that maize ZmBES1 / BZR1-5 gene can improve plant growth. The effect of kernel size on application. The maize ZmBES1 / BZR1-5 gene can be used to breed high-yield crops, and the maize ZmBES1 / BZR1-5 gene has broad application prospects in the field of plant grain development, especially in the field of high-yield plants, and its economic benefit potential is huge.

Description

technical field [0001] The invention relates to the application of maize ZmBES1 / BZR1-5 gene in cultivating large-grain plants and belongs to the field of genetic engineering. Background technique [0002] Both corn and rice are important food crops in my country, and high-yield breeding is one of the effective guarantees to ensure food security and sustainable agricultural development in my country. The yield of corn and rice is closely related to the grain weight of the grain, which refers to the weight of each seed, and the grain weight is largely determined by the size of the seed. Therefore, discovering genes that can increase grain weight has great application value for high-yield breeding of crops. In modern agriculture, increasing crop seed weight or grain size is key to crop improvement. In recent years, the research on the regulation mechanism of plant seed size has progressed rapidly, and it has become a hot spot and frontier in the field of plant research. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/10A01H6/20A01H6/46
CPCC07K14/415C12N15/8261
Inventor 于好强孙福艾付凤玲李晚忱曹洋丁磊
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com