Corn gene ZmLIP2 and application thereof

A corn and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of positioning research that has not been reported, and achieve the effect of huge economic benefit, reducing toxic effects, and enhancing growth conditions.

Active Publication Date: 2020-06-12
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there have been reports on the characteristics and possible mechanisms of lead adsorption by different maize materials and different tissues, there has been no report on the mapping of genes related to the control of heavy metal lead in maize.

Method used

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  • Corn gene ZmLIP2 and application thereof
  • Corn gene ZmLIP2 and application thereof
  • Corn gene ZmLIP2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Transcriptome Analysis of the Roots of Maize Inbred Line 178

[0027] 1-1. Lead stress treatment of corn inbred line materials

[0028] Select plump corn inbred line 178 seeds to be sterilized with 75% ethanol for 1 min, soak in 10℅ H2O2 solution for 15 min and shake continuously during disinfection, then rinse with deionized water for 3 to 5 times until the residual H2O2 is rinsed clean, and then use Soak in deionized water for 4 hours, and finally germinate with filter paper in the dark at 28°C. After about 2-3 days, transfer the germinated corn to a floating plate, and culture it with Hoagland nutrient solution under the conditions of light (16h) / darkness (8h) at 28°C for hydroponics, and change the culture medium twice a week. Six seedlings with consistent growth were selected for stress treatment. Remove the endosperm of these 6 seedlings and move them into plastic containers with nutrient solution. Two treatments were set up in this experiment: normal...

Embodiment 2

[0047] Example 2. The acquisition and analysis of ZmLIP2 interference strains

[0048] In this example, Ubi is used as the promoter, NOS is used as the terminator, bar is used as the selection marker gene, and kanamycin is used as the selection marker gene. The target gene fragment primers are designed according to the ZmLIP2 sequence, and the CUB vector is used as the backbone. Homologous recombination method , construct the interference vector of ZmLIP2 gene by double digestion with BamHI and SmalⅠ. Transform the constructed interference vector into Agrobacterium competent cells. The T0 generation ZmLIP2 gene interference maize was obtained by infecting wild-type maize C01 immature embryos with Agrobacterium.

[0049] The following are the specific steps:

[0050] 2-1. Extraction and purification of corn RNA

[0051] The total RNA of the maize inbred line Qi C01 (hereinafter also referred to as wild-type maize) was extracted by the Trizol method, and the specific method r...

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Abstract

The invention discloses a corn gene ZmLIP2 and an application thereof. ZmLIP2 gene transformation is performed on corns by using an agrobacterium-mediated transgenic technology, and an RNAi interference transformation event of the corn ZmLIP2 gene is obtained. An analysis on an expression quantity of RNAi-interfered plants shows that the expression quantity is remarkably reduced compared with thatof receptor materials. The gene can negatively regulate and control lead absorption and transport of plants, a relationship between the corn ZmLIP2 gene and lead stress resistance of the plants is clarified for the first time, and importance of the gene in a lead resistance application of the corns is verified.

Description

technical field [0001] The invention relates to a corn gene ZmLIP2 and its application, belonging to the field of plant genetic engineering. Background technique [0002] Corn is one of the most important food crops worldwide. However, with the continuous deterioration of the ecological environment, the content of heavy metals in the soil is increasing, and its growth and yield quality are seriously affected by abiotic stress including heavy metals, which seriously restricts crop yield. Excavating stress-related genes, studying their stress response mechanism, and cultivating new maize varieties with strong stress resistance are the most effective ways to cope with adversity stress and increase maize yield. At present, my country's maize stress-resistant germplasm resources are very scarce. It is not only a very difficult process to obtain stress-resistant germplasm resources through conventional breeding, but also the breeding cycle is relatively long, making it difficult ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8218C12N15/8271
Inventor 沈亚欧梁天虎张严玲陈珉
Owner SICHUAN AGRI UNIV
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