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Application of Gene zm00001d040827 in Improving Maize Yield

A corn and gene technology, applied in the field of molecular biology, can solve problems such as difficult problems and large number of genes

Active Publication Date: 2021-04-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The result of traditional QTL mapping is a specific segment, but due to the large number of genes in the segment, it is difficult for researchers to directly determine the genes that actually control traits

Method used

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  • Application of Gene zm00001d040827 in Improving Maize Yield
  • Application of Gene zm00001d040827 in Improving Maize Yield
  • Application of Gene zm00001d040827 in Improving Maize Yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Genome-wide association analysis was used to identify the gene Zm00001d040827 controlling the 100-kernel weight of maize

[0024] 1-1 Association analysis population planting and phenotype data acquisition

[0025] The association analysis population containing 315 inbred lines was planted in Hongya of Sichuan, Jinghong of Yunnan and Ya'an of Sichuan according to the random block standard, and two replicates were set for each environment. Each inbred line is planted in two rows, the row length is 3 meters, and the distance between rows is 0.8 meters. 14 plants are planted in each row, and the density is about 58,000 plants / ha. Field planting and management are the same as conventional corn planting. Harvest and plant after maturity. For each inbred line, 100 grains were randomly selected to measure the weight, and the average value of three measurements was taken as the phenotype value of the inbred line. The statistical software SPSS 20.0 was used to evalu...

Embodiment 2

[0030] Example 2. Association analysis and haplotype identification of candidate gene Zm00001d040827

[0031] The DNA of 315 materials in the association analysis population was extracted by CTAB method. Primers were designed according to the DNA sequence and promoter region (2000bp) of the gene Zm00001d040827, and PCR amplification was performed.

[0032] Primer information for amplifying the promoter region:

[0033]

[0034]

[0035] DNA amplification sequence system:

[0036]

[0037] The PCR amplification procedure is as follows:

[0038]

[0039] A total of 205 markers were found in the amplification results, including 17 Indels and 188 SNPs variation sites. Combined with 205 markers and 100-seed weight phenotype for candidate gene association analysis (model: MLM; P = 0.05), the results showed that three markers: SNP-3-68160672, SNP-3-68159986, SNP-3-68159004 were in It is significant in Hongya of Sichuan, Jinghong of Yunnan, Ya'an of Sichuan and best li...

Embodiment 3

[0040]Example 3. Genetic transformation and homozygosity of excellent haplotype of gene Zm00001d040827 in maize

[0041] 3-1 RNA extraction and CDNA acquisition of excellent haplotype materials

[0042] Select excellent haplotype materials with plump seeds, put them evenly in the cleaned quartz sand, and cultivate them in a 28°C incubator. When the seedlings reached the 3-leaf stage, fresh leaves were taken to extract RNA. The extraction method was carried out according to the Invitrogen Trizol kit manual. Extracted RNA using TakaRa's RT reagent Kit (Perfect Real Time) reverse transcription into cDNA, the reaction system and procedures are as follows:

[0043] Genomic DNA removal reaction (Total RNA dosage: max. 1 μL):

[0044]

[0045] Reverse transcription reaction system:

[0046]

[0047] 3-2 CDS region amplification of gene Zm00001d040827

[0048] (1) Primer design: Primers were designed in Primer 5.0 according to the CDS region sequence of the candidate gene...

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Abstract

The invention discloses the application of the gene Zm00001d040827 in increasing the yield of corn. The invention determines the relationship between the Zm00001d040827 gene and the yield of corn. The Zm00001d040827 gene can increase the chlorophyll content of leaves, increase the weight of 100 kernels of corn, and increase the yield of corn per ear, thereby increasing the yield of corn. Yield. The invention verifies the importance of the gene in improving the yield of corn, and lays a theoretical basis for the application of the gene in improving the yield of corn.

Description

technical field [0001] The invention relates to the application of gene Zm00001d040827 in improving corn yield, belonging to the field of molecular biology. Background technique [0002] Corn is a multiple crop of food, economics and feed, and plays an important role in national production and life. Therefore, continuously increasing corn production has practical significance and strategic prospects. As an important yield component, 100-kernel weight is a key factor in the formation of maize yield. With the rapid development of molecular biology, it is a simple, fast and efficient breeding strategy to excavate, verify and integrate genes controlling 100-grain weight in plants. [0003] At present, the genetic analysis of 100-kernel weight in maize mainly focuses on the preliminary mapping of QTLs, and there are few reports on fine mapping and cloning verification of genes related to 100-kernel weight in maize. [0004] With the advancement of sequencing technology, the dev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H5/12A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 沈亚欧马浪浪刘鹏张敏燕郑艳付俊
Owner SICHUAN AGRI UNIV
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