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A method of using magnetic field to promote high production of antimicrobial peptides by Bacillus amyloliquefaciens

A technology of amylolytic spores and antimicrobial peptides, applied in high-yield antimicrobial peptides and biological fields, to achieve the effects of reducing production costs, simple structure, and convenient magnetic field treatment

Active Publication Date: 2019-02-01
山东华辰制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of magnetic field to increase the production of antimicrobial peptides from Bacillus amyloliquefaciens

Method used

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  • A method of using magnetic field to promote high production of antimicrobial peptides by Bacillus amyloliquefaciens
  • A method of using magnetic field to promote high production of antimicrobial peptides by Bacillus amyloliquefaciens
  • A method of using magnetic field to promote high production of antimicrobial peptides by Bacillus amyloliquefaciens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Such as Figure 4 The shown fermentation culture device for promoting the high production of antimicrobial peptides by Bacillus amyloliquefaciens includes a fermenter body 1, and the fermenter body 1 is provided with a circulation reflux device, and a magnetic induction coil 2 is provided on the circulation reflux device.

[0049] The circulation return device includes a circulation return pump 3 and a circulation return pipeline 4 , and a liquid flow meter 5 is arranged on the circulation return pipeline 4 .

[0050] The fermenter body 1 is provided with a dissolved oxygen detection device 6 and a pH detection device 7 .

[0051] A kind of method utilizing magnetic field to promote Bacillus amyloliquefaciens high-production antimicrobial peptide, the steps are as follows:

[0052] (1) Inoculate the activated Bacillus amyloliquefaciens into the seed medium, and culture the seeds for 18 hours at 35°C and 200 rpm to obtain the seed solution;

[0053]In described step (1...

Embodiment 2

[0083] As described in Example 1, the method of utilizing a magnetic field to promote high-production antimicrobial peptides by Bacillus amyloliquefaciens, the difference is that the steps are as follows:

[0084] (1) Inoculate the activated Bacillus amyloliquefaciens into the seed medium, and culture the seeds for 16 hours at 40°C and 320rpm to obtain the seed solution;

[0085] In described step (1), every liter of component of seed culture medium is as follows:

[0086] Starch 20g, yeast powder 5g, potassium phosphate 4g, magnesium sulfate 3g, manganese sulfate 0.001g, pH 6.5, dilute to 1L with deionized water;

[0087] (2) Transfer the seed liquid prepared in step (1) to the fermentation medium at a ratio of 5% (volume percentage), and ferment for 10 hours initially at 25°C, stirring speed 100rpm, and air flow 0.5vvm. The cells entered the logarithmic growth phase, and then under the conditions of 25°C, stirring speed of 100rpm, and air flow rate of 0.5vvm, they were ferm...

Embodiment 3

[0110] A kind of method utilizing magnetic field to promote Bacillus amyloliquefaciens high-production antimicrobial peptide, the steps are as follows:

[0111] (1) Inoculate the activated Bacillus amyloliquefaciens into the seed medium, and culture the seeds for 24 hours at 25°C and 100 rpm to obtain the seed liquid;

[0112] In described step (1), every liter of component of seed culture medium is as follows:

[0113] Glucose 40.0g, yeast powder 20.0g, potassium phosphate 1.0, magnesium sulfate 0.5g, manganese sulfate 0.001g, pH 6.5, deionized water to 1L;

[0114](2) Transfer the seed solution prepared in step (1) to the fermentation medium at a ratio of 1% (volume percentage), and ferment for 8 hours at 30° C., stirring speed 180 rpm, and air flow 0.8 vvm, and the thalline The cells enter the logarithmic growth phase, and then under the conditions of 30°C, stirring speed 180rpm, and air flow rate 0.8vvm, they undergo a magnetic field fermentation treatment with a current ...

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Abstract

The invention relates to a method and device for improving an antibacterial peptide yield of bacillus amyloliquefaciens by a magnetic field. The method comprises 1, inoculating a seed medium with activated bacillus amyloliquefaciens and carrying out seed culture to obtain a seed liquid, 2, transferring the seed liquid obtained by the step 1 into a fermentation medium, carrying out preliminary fermentation until bacterial cells are in a logarithmic phase, carrying out continuous or discontinuous magnetic field fermentation, and carrying out follow-up fermentation treatment to obtain the antibacterial peptide-containing broth. The invention also relates to a device for magnetic field fermentation treatment. The bacillus amyloliquefacien cells in a logarithmic phase are treated in a magnetic field so that a desired product antibacterial peptide yield and antibacterial peptide activity are substantially improved. The method has simple processes, can be operated easily and substantially reduces an antibacterial peptide production cost.

Description

technical field [0001] The invention relates to a method and a device for promoting high production of antimicrobial peptides by Bacillus amyloliquefaciens by using a magnetic field, and belongs to the technical field of biotechnology. Background technique [0002] Antimicrobial peptides is an endogenous polypeptide produced by the innate immune defense system of organisms, which exists in almost all kinds of organisms from prokaryotes to humans, and its molecular weight is mostly between 1000-7000, composed of 10- 100 amino acid residues. Antimicrobial peptides generally play a bactericidal role by destroying the cell membrane to cause the leakage of cell contents. Compared with traditional antibiotics, antimicrobial peptides are not easy to produce drug resistance, have high safety, can kill multi-drug resistant bacteria, and have no residue. It has great application potential in fields such as pharmaceutical industry, livestock and poultry breeding, and food preservation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12M1/42C12M1/34C12R1/07
Inventor 姜正军陈溥言刘海玉匡宝晓王少娟周文福别怀周于海燕
Owner 山东华辰制药有限公司
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