Method for increasing leaf number and biomass of tobacco by utilizing tobacco cytochrome C gene NtCYP94B3s

A technology of cytochrome and C gene, which is applied in the field of genetic engineering, can solve the problems of long breeding cycle and limited resources, and achieve the effects of increasing content, broad application prospects, and huge economic benefit potential

Inactive Publication Date: 2020-12-18
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

[0004] At present, the method of increasing tobacco leaf number and biomass is mainly to screen varieties with excellent plant type and leaves through conventional breeding. The breeding cycle is long and the resources are limited

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  • Method for increasing leaf number and biomass of tobacco by utilizing tobacco cytochrome C gene NtCYP94B3s
  • Method for increasing leaf number and biomass of tobacco by utilizing tobacco cytochrome C gene NtCYP94B3s
  • Method for increasing leaf number and biomass of tobacco by utilizing tobacco cytochrome C gene NtCYP94B3s

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the embodiments and accompanying drawings, but the present invention is not limited in any way. Any transformation or replacement based on the teaching of the present invention belongs to the protection scope of the present invention.

[0020] The method for increasing the number and biomass of tobacco leaves by using tobacco cytochrome C gene NtCYP94B3s is characterized in that the nucleotide sequence of said tobacco cytochrome C gene NtCYP94B3s is as shown in the sequence listing SEQ ID NO: 1, or the same as the sequence listing A nucleotide sequence that hybridizes to the DNA sequence defined by SEQ ID No: 1, or a DNA sequence that has more than 70% homology with the DNA sequence defined by SEQ ID No: 1 in the sequence listing and encodes the same functional protein. The amino acid sequence encoded by the tobacco cytochrome C gene NtCYP94B3s is shown in SEQ ID NO: 2 in the sequence listing.

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Abstract

The invention relates to a method for increasing the leaf number and the biomass of tobacco by utilizing a tobacco cytochrome C gene NtCYP94B3s. The nucleotide sequence of the tobacco cytochrome C gene NtCYP94B3s is shown as SEQ ID NO:1 in a sequence table. The method comprises the following steps of: firstly, constructing a plant RNAi vector, then, identifying agrobacterium tumefaciens-mediated tobacco transformation and transgenic plants, then, extracting the total RNA of wild plants and 25 pHellsgate12-transformed RNA gene T0-generation plants, performing Real time-PCR analysis with reference genes of 26s, analyzing expression conditions of different strains, and selecting two plants with the lowest expression quantity. According to the method in the invention, inhibition of the expression of the tobacco endogenous gene NtCYP94B3s in tobacco plants can obviously improve the leaf number and the biomass of the tobacco. The method has a wide application prospect and huge economic benefit potential.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an application of tobacco cytochrome C gene NtCYP94B3s. Background technique [0002] Tobacco is an important economic crop. Tobacco contains about 1% to 9% of alkaloids and rutin, organic acids, fats, resins, proteins, sugars, spices and other substances. It is widely used in the fields of chemical industry, pesticide and medicine. Tobacco has strong physiological activity, and there are many records of using tobacco to treat diseases in traditional Chinese medicine. Modern technology has achieved many results in tobacco research. Nicotine can be made into smoking cessation preparations by appropriate method and dosage, such as smokeless tobacco, nicotine chewing gum, nicotine patch, nicotine spray and so on. In terms of disease prevention and treatment, using the extensive effects of nicotine on the central nervous system, through the identification o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/12A01H6/82
CPCC12N15/8218C12N15/8243C12N15/8261C12N9/0071C12Y114/14
Inventor 杨大海王蕾杨飞汤金香白戈李勇逄涛姚恒谢贺陈学军肖炳光费明亮张谊寒
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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