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Application of corn ZmbZIP107 gene in cultivation of lead stress-resistant plants

A technology of transgenic plants and corn, which is applied in the field of plant response to heavy metal stress, can solve problems such as no lead tolerance, and achieve the effect of huge economic benefit potential and broad application prospects

Active Publication Date: 2017-12-19
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, maize bZIP gene has been found to be related to maize disease and salt resistance, but there is no report related to lead tolerance

Method used

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  • Application of corn ZmbZIP107 gene in cultivation of lead stress-resistant plants
  • Application of corn ZmbZIP107 gene in cultivation of lead stress-resistant plants
  • Application of corn ZmbZIP107 gene in cultivation of lead stress-resistant plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. Expression analysis of maize ZmbZIP107 gene under lead stress

[0018] 1-1. Lead stress treatment of maize inbred line materials

[0019] Select the plump 312 corn inbred line seeds and sterilize them with 75% ethanol for 1 min, 10℅ H 2 O 2 Soak the solution for 15 minutes and shake it continuously during disinfection, then rinse with deionized water 3 to 5 times until the residual H 2 O 2 Rinse well, soak in deionized water for 4 hours, and finally germinate with filter paper in the dark at 28°C. After about 2-3 days, transfer the germinated corn to a floating plate, and culture it with Hoagland nutrient solution at 28°C under light (16h) / dark (8h) conditions for hydroponic culture. Change the culture medium twice a week until the seedlings grow. To the three-leaf stage, stress treatment.

[0020] 1-2. Lead stress treatment:

[0021] Select 6 seedlings with the same growth and remove the endosperm and transfer them into a plastic container filled with nutrient solu...

Embodiment 2

[0043] Example 2. Analysis of lead tolerance of Arabidopsis homologous gene mutant plants

[0044] For the Arabidopsis homologous gene mutant SALK_028204 (ordered from the Arabidopsis TAIR mutant library), first use 75% absolute ethanol to disinfect the mutant and wild-type seeds for 1 min, then disinfect with 1% NaClO for 15 min, and finally with no After washing with bacteria water for 3 to 5 times, sowing them on 1 / 2MS medium and culturing for 7 days, and then transplant wild-type plants and mutant plants at 0g / L and 0.15g / L Pb(NO 3 ) 2 Continue culturing on 1 / 2MS medium, and observe the plant growth after 10 days. The results are as follows figure 2 with image 3 As shown, the growth of mutant plants under normal conditions is not much different from that of wild type. Under lead stress conditions, lead stress obviously inhibits the growth of plant roots, but the growth of mutants is more significantly inhibited. The average root length of wild-type plants was reduced by 45% ...

Embodiment 3

[0046] Example 3. QTL localization verification.

[0047] Using the constructed corn IBM Syn 10DH (B73×Mo17) population of 200 families as the linkage population, the processing method and phenotype identification are the same as 1-2, and then combined with 6618 bin marker genotype data, the QTL mapping results are as follows Figure 4 As shown, a QTL locus related to the total number of roots was detected on chromosome 10, with a phenotypic contribution rate of 5.1%, located in the segment chr10.471~chr10.596.5, and the corresponding physical position on the chromosome is 39.075 Mb ~ 53.375Mb (Maize B73RefGen_v3). The ZmbZIP107 gene falls in the QTL mapping segment, further verifying the importance of this gene in the process of lead stress in maize.

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Abstract

The invention relates to an application of a corn ZmbZIP107 gene in cultivation of lead stress-resistant plants. Through qRT-PCR detection, the ZmbZIP107 gene enables inducible expression under lead stressed, and expression level of tolerant inbred line root is obviously higher than the expression level of sensitive inbred line roots. According to the invention, an arabidopis thaliana homologous gene mutant is used for researching, under lead stressed, the growth vigor of Columbia wild type is obviously better than that of a mutant, and the inhibition degree of the wild type is lower than that of the mutant. The ZmbZIP107 gene is located in a QTL section on No.10 chromosome, the gene enables inducible expression after the plant is subjected to lead stressing, and positive regulation and control can be carried out on responding lead stress by the plant, so that the lead stress-resistant capability of the plant is increased. The invention firstly clears the relation between the corn ZmbZIP107 gene and the salt stress-resistance of the plant, and the importance of the gene in the corn lead-resistant application is verified. The application provides theoretical basis and utilization value of the gene on corn and other plants to increase the stress resistance.

Description

Technical field [0001] The present invention mainly relates to the field of plant response to heavy metal stress, and specifically relates to the application of the maize ZmbZIP107 gene in cultivating plants resistant to lead stress Background technique [0002] Corn is one of the most important food crops in the world. However, as the ecological environment continues to deteriorate, the content of heavy metals in the soil is increasing, and its growth and yield quality are severely affected by non-biological stress including heavy metals, which severely restricts crop yields. Excavating genes related to stress resistance, studying its response mechanisms to stress, and cultivating new corn varieties with strong stress resistance are the most effective ways to deal with stress and increase corn yield. At present, my country's corn stress-resistant germplasm resources are very scarce. It is not only a very difficult process to obtain stress-resistant germplasm resources through c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/10
CPCC07K14/415C12N15/8271
Inventor 沈亚欧张严玲张晓祥
Owner SICHUAN AGRI UNIV
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