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Application of maize zmbzip107 gene in breeding plants resistant to lead stress

A technology of transgenic plants and corn, which is applied in the field of plant response to heavy metal stress, can solve problems such as no lead tolerance, and achieve the effect of huge economic benefit potential and broad application prospects

Active Publication Date: 2019-12-03
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, maize bZIP gene has been found to be related to maize disease and salt resistance, but there is no report related to lead tolerance

Method used

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  • Application of maize zmbzip107 gene in breeding plants resistant to lead stress
  • Application of maize zmbzip107 gene in breeding plants resistant to lead stress
  • Application of maize zmbzip107 gene in breeding plants resistant to lead stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. Expression analysis of maize ZmbZIP107 gene under lead stress

[0018] 1-1. Lead stress treatment of corn inbred line materials

[0019] Select plump 312 corn inbred seeds and sterilize with 75% ethanol for 1min, 10℅ of H 2 o 2 The solution was soaked for 15 minutes and kept shaking during disinfection, and then rinsed with deionized water for 3 to 5 times until the residual H 2 o 2 Rinse well, then soak in deionized water for 4 hours, and finally germinate with filter paper under 28°C dark conditions. After about 2-3 days, transfer the germinated corn to a floating plate, and culture it with Hoagland nutrient solution under the conditions of light (16h) / darkness (8 h) at 28°C for hydroponics, change the culture medium twice a week, and wait for the seedlings to grow. Stress treatment was carried out at the three-leaf stage.

[0020] 1-2. Lead stress treatment:

[0021] Select 6 seedlings with consistent growth, remove the endosperm, and move them into ...

Embodiment 2

[0043] Embodiment 2. Arabidopsis homologous gene mutant plant lead tolerance analysis

[0044] The Arabidopsis homologous gene mutant SALK_028204 (ordered from the Arabidopsis TAIR mutant library), first sterilized the mutant and wild-type seeds with 75% absolute ethanol for 1 min, then sterilized with 1% NaClO for 15 min, and finally sterilized with no After washing with bacterial water for 3 to 5 times, they were sown on 1 / 2 MS medium and cultured for 7 days, and then the wild-type plants and mutant plants were transplanted in 0g / L and 0.15g / L Pb(NO 3 ) 2 Continue to cultivate on the 1 / 2MS medium, observe the plant growth status after 10 days, the results are as follows: figure 2 with image 3 As shown, under normal conditions, the growth of the mutant plants was not much different from that of the wild type. Under lead stress conditions, lead stress significantly inhibited the growth of plant roots, but the growth of the mutants was more significantly inhibited. The ave...

Embodiment 3

[0046] Example 3. QTL positioning verification.

[0047] Using 200 families of the established corn IBM Syn 10DH (B73×Mo17) population as a linkage population, the processing method and phenotype identification are the same as 1-2, and combined with 6618 bin marker genotype data, the QTL mapping results are as follows: Figure 4 As shown, a QTL site related to the total root number of the root system was detected on chromosome 10, with a phenotype contribution rate of 5.1%, located in the segment chr10.471~chr10.596.5, and the corresponding physical position on the chromosome was 39.075 Mb ~ 53.375Mb (Maize B73RefGen_v3). ZmbZIP107 gene falls in the segment of this QTL mapping, which further verifies the importance of this gene in the process of lead stress in maize.

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Abstract

The invention relates to an application of a corn ZmbZIP107 gene in cultivation of lead stress-resistant plants. Through qRT-PCR detection, the ZmbZIP107 gene enables inducible expression under lead stressed, and expression level of tolerant inbred line root is obviously higher than the expression level of sensitive inbred line roots. According to the invention, an arabidopis thaliana homologous gene mutant is used for researching, under lead stressed, the growth vigor of Columbia wild type is obviously better than that of a mutant, and the inhibition degree of the wild type is lower than that of the mutant. The ZmbZIP107 gene is located in a QTL section on No.10 chromosome, the gene enables inducible expression after the plant is subjected to lead stressing, and positive regulation and control can be carried out on responding lead stress by the plant, so that the lead stress-resistant capability of the plant is increased. The invention firstly clears the relation between the corn ZmbZIP107 gene and the salt stress-resistance of the plant, and the importance of the gene in the corn lead-resistant application is verified. The application provides theoretical basis and utilization value of the gene on corn and other plants to increase the stress resistance.

Description

technical field [0001] The present invention mainly relates to the field of plants responding to heavy metal stress, in particular to the application of corn ZmbZIP107 gene in cultivating lead stress-tolerant plants Background technique [0002] Corn is one of the most important food crops worldwide. However, with the continuous deterioration of the ecological environment, the content of heavy metals in the soil is increasing day by day, and its growth and yield quality are seriously affected by abiotic stress including heavy metals, which seriously restricts crop yield. Excavating stress-related genes, studying their stress response mechanism, and cultivating new maize varieties with strong stress resistance are the most effective ways to cope with adversity stress and increase maize yield. At present, my country's corn stress-resistant germplasm resources are very scarce. It is not only a very difficult process to obtain stress-resistant germplasm resources through conven...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/10A01H6/46A01H6/20
CPCC07K14/415C12N15/8271
Inventor 沈亚欧张严玲张晓祥
Owner SICHUAN AGRI UNIV
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