Co-dominant SSR (simple sequence repeat) markers closely interlinked with major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum and application of co-dominant SSR markers

Abstract

The invention discloses co-dominant SSR (simple sequence repeat) markers closely interlinked with a major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum and application ofthe co-dominant SSR markers. The serial numbers of the co-dominant SSR markers closely interlinked with the major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum are TM22038, TM23004 and TM22041, and nucleotide sequences of PCR (polymerase chain reaction) amplification products of the markers are respectively shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 and SEQ IDNo.4, SEQ ID No.5 and SEQ ID No.6. The application refers to application of the co-dominant SSR markers closely interlinked with the major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum in detecting whether nicotiana tabacum genome DNA (deoxyribonucleic acid) has a homozygous genotype nic1 or not and a nicotiana tabacum variety of a low nicotine content is available or not. The co-dominant SSR markers disclosed by the invention have the characteristics of being stable, reliable, simple and convenient, rapid and low in cost, so that the molecular markers can be applied to assistant selection of molecular markers of the low nicotine content gene nic1 in high-quality nicotiana tabacum breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a co-dominant SSR marker closely linked with tobacco nicotine synthesis main regulation gene nic1 and its application. Background technique [0002] Tobacco (Nicotiana tabacum L.) is an important economic crop and is an annual herb belonging to the family Solanaceae. Nicotine is an important characteristic compound of cultivated tobacco, accounting for about 95% of the total alkaloids in tobacco, and its content in tobacco leaves directly determines the quality and commercial use of tobacco. At present, major tobacco companies and research institutions have begun to actively cultivate tobacco varieties with low nicotine content and develop tobacco products with low nicotine content. Tobacco companies and research institutions at home and abroad are working to identify the main regulatory gene NIC1 for nicotine synthesis, hoping to use this gene to regulate the nicotine c...

AI Technical Summary

Problems solved by technology

[0005] So far, although studies on tobacco nicotine synthesis genes have been reported, the existing research results are all based on the genetic background of Burley tobacco, and the only one report of a marker linked to the NIC1 gene is based on SNP variation The dCAPS marker developed by the site has serious shortcomings in tobacco breeding practice and tobacco leaf production: 1) There are serious limitations in the scope of use, that is, the scope of use of the dCAPS marker for the detection and identification of the NIC1 gene that has been reported and applied for a patent is relatively small , which is only applicable to the type of Burley tobacco; in addition to the type of Burley tobacco in cultivated tobacco, there are many different types of flue-cured tobacco, oriental tobacco, cigar tobacco, air-dried tobacco, sun-cured tobacco, etc. Or species that cannot be detected and identified by the dCAPS marker

2) The detection system is cumbersome and time-consuming. The actual detection of dCAPS markers involves PCR amplification, selection of restriction endonucleases or combinations, digestion of PCR amplification products, and electrophoretic separation.

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