Tobacco JAZ protein gene and application thereof in reducing tobacco nicotine content

A tobacco and protein technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as easy thinking and unclear molecular mechanism.
CN102993286AInactive Publication Date: 2013-03-27BEIJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING NORMAL UNIVERSITY
Publication Date
2013-03-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a tobacco JAZ protein gene and application thereof in reducing tobacco nicotine content. The gene is a tobacco JAZ protein gene and is named as NtJAZ7, the protein coded with the gene has a typical TIFY structure field and a jas structure field, and the gene is subjected to induced expression of jasmonate. Through constructing an RNAi gene transfer vector of the NtJAZ7 gene, and transforming the tobacco, the expression of NtJAZ7 in tobacco cells is successively inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with the wild type tobacco. The invention provides an important scientific basis and a technological support for breeding low-nicotine content tobacco.
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Description

technical field

[0001] The invention relates to the synthesis of tobacco nicotine and related genes for regulation, in particular to the application of the gene for regulating the synthesis of tobacco nicotine in reducing the content of tobacco nicotine. Background technique

[0002] Smoking seriously endangers people's health, so how to reduce the harm of smoking has become one of the topics that governments, medical experts, and botanists have paid close attention to and studied. Many of the world's top tobacco companies have invested heavily in research on the metabolic pathways and regulatory mechanisms of tobacco secondary metabolites. Nicotine is a pyridine alkaloid mainly found in Nicotiana plants of the family Solanaceae, and is an important secondary metabolite in tobacco. Current research on nicotine biosynthesis mainly focuses on cloning enzymes related to its metabolism and analyzing its physiological and biochemical properties. Nicotine is formed in the roots ...

Claims

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