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Method for inducing tobacco nicotine conversion in advance and use thereof

A nicotine and tobacco technology, applied in the direction of recombinant DNA technology, cells modified by introducing foreign genetic material, etc., to achieve the effects of improving the transformation rate, improving the aroma and safety of tobacco leaves, and reducing the transformation rate of nicotine

Inactive Publication Date: 2007-11-14
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is aimed at the defects of the above-mentioned existing methods for inducing nicotine conversion, and provides a convenient and efficient method for inducing tobacco nicotine conversion in advance and its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select 20 B21 highly transformed strains and 20 non-transformed strains, remove a piece of lower tobacco leaf, spray 0.5% 3,5-dinitrosalicylic acid, and air-cure in an air-drying room at a temperature of 16-23°C for 6 days. Put it into the oven for 5 minutes at 105°C, then dry it at 60°C, measure nicotine and nornicotine, and judge whether it is a transformed strain according to the conversion rate.

Embodiment 2

[0024] Select the tobacco strain in Example 1, remove a piece of lower tobacco leaf, spray 0.5% 3,5-dinitrosalicylic acid, put it into an oven after covering plastic bags, and perform moisturizing treatment for 6 days at 38°C ± 1°C. Then, it was dried in an oven at 105°C for 5 minutes, dried at 60°C, and nicotine and nornicotine were measured, and whether it was a transformed strain was judged according to the conversion rate.

Embodiment 3

[0026] Select the tobacco plant in Example 1, take a piece of tobacco leaf in the lower part of the tobacco plant to be tested, spray 0.5% 3,5-dinitrosalicylic acid on the surface of the tobacco leaf, and keep 80% ± 1% in a constant temperature and humidity chamber. Humidity at 38°C±0.1°C for 6 days, put in an oven for 5 minutes at 105°C, then dry at 60°C, measure nicotine and nornicotine, and judge whether it is a transformed strain according to the conversion rate.

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PUM

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Abstract

A guide tobacco smoke soda moves up transformation method and its application, its features are: the invention of the induction methods include the following steps: from the bottom 8-12 leaf tobacco plant leaves a bit, spraying concentration of 0.1% -10% of the 3, 5 - dinitrosalicylic acid in the environment temperature of 15 degree C -43 degree C under modulation processing time than four days to induce the conversion of nicotine into characters in the expression of green leaves in early. The induction method in tobacco breeding and seed breeding in the identification of nicotine into the application through the drop of nicotine and nicotine content, according to determine whether the conversion rate into line. The present invention has the advantage of being: May remain plants the warning from past example to decide on the smoke alkali to transform and to pick out ahead of time, achieves the purification seed's effect, may cut descendant's smoke alkali conversion rate effectively, regarding enhances the leaf tobacco fragrance and the security has the vital significance.

Description

technical field [0001] The present invention relates to the field of tobacco planting and breeding, in particular to a method for inducing the early conversion of tobacco nicotine and its application, mainly through inducing the early conversion of tobacco nicotine to nornicotine to carry out the nicotine-transformed strain in the process of breeding improved tobacco varieties Identification. Background technique [0002] The composition and content of tobacco alkaloids are the core of tobacco leaf quality, and have a direct impact on the physiological strength, flavor and content of harmful substances in cigarettes, especially tobacco-specific nitrosamines (TSNAs). TSNAs exist in tobacco and smoke, mainly including N-nitrosonornicotine (NNN), 4-(N-methylnitrosamine)-1-(3-pyridyl)-1-butane ketone (NNK), N-nitrosobasine (NAB) and N-nitrosoannicotine (NAT). Under normal circumstances, tobacco alkaloids are dominated by nicotine, which accounts for more than 92% of the total ...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/09
Inventor 蔡斌杨军尹启生宋纪真薛超群
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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