Method for early evoking and identifying transformant of tobacco nicotine

Abstract

The invention discloses a method of early inducement identification of converter plants by tobacco smoke alkaloid. Plants are fixed and leaves are picked; any two kinds of or all of potassium bicarbonate, sodium bicarbonate and sodium chloride are mixed at the volume ratio of 1:1 or 1:1:1. The mixture is sprayed, air cured and dried. Nicotine and nornicotine are measured with a gas chromatograph; and the content of the nornicotine in the total quantity of the nicotine and the nornicotine is calculated. If the content of the nornicotine is less than 5 percent, the plants are not converter plants. If the percent conversion of the nicotine is larger than 5 percent, the plants are converter plants; and if the percent conversion of the nicotine is larger than 20 percent, the plants are high converter plants. The technical proposal of the invention has the following advantages: 1. the method adopted by the invention can ensure conversion to be completed within four days; the time is short, and the efficiency is high; 2. the chemical substances adopted by the invention are gentle, and have excellent effect; 3. the chemical substances adopted by the invention are environmentally friendly; 4. the cost of the chemical substances adopted by the invention is far below the cost of the processing method adopting ethephon.

Description

Technical field [0001] The invention relates to a method for early induction and identification of tobacco nicotine transformants. Background technique [0002] Alkaloids are a class of important chemical components contained in tobacco plants, which mainly include nicotine, nornicotine, neonicotine and pseudoequisetine. N. tabacum L and N. rustica L, which are valuable for cultivation, are both nicotine accumulation types, and their nicotine content accounts for more than 94% of the total alkaloid content. However, in the tobacco plant population of cultivars, individual plants have the ability to demethylate nicotine due to genetic mutations, resulting in a significant decrease in nicotine content and a corresponding increase in nornicotine content, which has the ability to convert nicotine to nornicotine The tobacco strain is called transformant. Because the conversion of nicotine is closely related to the change of tobacco flavor and the increase of tobacco nitrosamine conten...

AI Technical Summary

Problems solved by technology

Due to the poor flavor of this kind of tobacco leaves, they are classified separately when purchased, and the price is extremely low

Burley tobacco TN90 strains with different nicotine conversion abilities have no significant difference in agronomic traits, but the flavor quality of tobacco leaves decreases significantly with the increase of nornicotine content. Transformed tobacco leaves generally decline in Burley tobacco style, with abnormal flavor and serious oral residues

[0007] 2 Effects on TSNA formation in tobacco leaves

Although this method is effective, there are the following disadvantages: the one is that the time is long

The second is that the induction effect of ethephon treatment is relatively low, mainly because ethephon is a hormone release agent, which has strict requirements on the concentration and is not easy to accurately grasp. It often occurs that the treatment effect is inconsistent due to uneven spraying. Partially necrotic, too low to be effective, therefore, low transformants with low transformation capacity are often not identified

Third, ethephon has an impact on the environment and is polluting

Four is high cost