Genes capable of regulating and controlling nicotine content of tobacco and application of genes

A technology that regulates genes and nicotine, applied in the field of botany, can solve problems that affect nicotine content and are not completely thorough

Inactive Publication Date: 2015-03-04
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The synthesis and transport of tobacco nicotine are regulated by many factors. At present, the research on the anabolic pathway and transport of nicotine from the molecular point of view has not been completely thorough. Although some key genes in the nicotine synthesis pathway have been identified and cloned, such as QPT , PMT, MPO, etc. (Dwey and Xie, 2013, Phytochemistry; Sierro, 2014, Nature Communication), but there is no report on the regulation of nicotine synthesis and transport key genes through miRNA, thereby affecting nicotine content

Method used

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  • Genes capable of regulating and controlling nicotine content of tobacco and application of genes
  • Genes capable of regulating and controlling nicotine content of tobacco and application of genes
  • Genes capable of regulating and controlling nicotine content of tobacco and application of genes

Examples

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Effect test

Embodiment 1

[0052] Based on high-throughput sequencing to obtain the small RNA sequences of tobacco roots and leaves after topping and leaf damage, as well as the genome sequences of two ancestral species of tobacco, a large number of tobacco gene sequences can be found, which can form a stable hairpin structure, and its stem can Production or expression of small RNA sequences (mature sequences). The hairpin structures formed by 21 gene sequences are shown in Figure 1-21 . These predicted hairpin structures were stable (all MEF values ​​were less than -21.60). The expression sequences of these genes were detected, especially in the roots and leaves of topping and leaf damage tobacco, which indicated that the expression of these genes was probably induced by these treatments.

Embodiment 2

[0054] The small RNA sequences generated by the expression of the above 21 genes are predicted by the target genes, and all target the key protein-coding genes in the tobacco nicotine synthesis and transport pathway, including QPT (quinolinate phosphirobosyltransferase), PMT (putrescine N-methyltransferase), MATE (multi antimicrobial extrusion family protein) and other genes (Table 1). A large number of studies have shown that these genes are the key functional genes of nicotine synthesis (QPT and PMT) and transport (MATE), respectively (Dwey and Xie, 2013, Phytochemistry). The predicted values ​​of the 21 genes and their target genes found in the present invention are all between 0-3.0 (generally, the close value is 3.0, exceeding this value indicates that the binding result is unreliable), and all target genes are regulated by cleavage. At the same time, the analysis results of the degradome data obtained in the experiment showed that 14-21 cut fragments had been detected in...

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Abstract

The invention discloses genes capable of regulating and controlling nicotine content of a tobacco and application of the genes. A sequence of the genes capable of regulating and controlling the nicotine content of the tobacco is any one as shown in SEQ ID: No.1-21. The application of the genes is as follows: the genes capable of regulating and controlling the nicotine content of the tobacco are used for regulating synthesis and translocation amount of nicotine of the tobacco, so as to realize regulating and controlling of nicotine content in the tobacco. 21 genes obtained in the invention can form a stable hairpin structure, and analysis on the obtained tobacco non-coding small RNA sequence shows that part of small RNAs are from a sequence of a stem of the tobacco; and the small RNAs predict that target genes of the small RNAs are respectively targeted to a tobacco nicotine synthesis and transportation key protein coding gene. An miRNA regulating and controlling mechanism obtained in the invention can provide a deeper understanding for a role played by a key gene for a synthesis and translocation route of nicotine in tobacco, so that nicotine content character of the tobacco can be better improved.

Description

technical field [0001] The invention belongs to the technical field of botany, and in particular relates to a tobacco nicotine content regulating gene and its application. Background technique [0002] The synthesis and transport of tobacco nicotine are regulated by many factors. At present, the research on the anabolic pathway and transport of nicotine from the molecular point of view has not been completely thorough. Although some key genes in the nicotine synthesis pathway have been identified and cloned, such as QPT , PMT, MPO, etc. (Dwey and Xie, 2013, Phytochemistry; Sierro, 2014, Nature Communication), but there is no report on the regulation of nicotine synthesis and transport key genes through miRNA, thereby affecting nicotine content. Therefore, this patent, as an important part of the nicotine synthesis and transport pathway, plays an important role in improving tobacco nicotine content and the like. Contents of the invention [0003] The first object of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A01H5/00
Inventor 肖炳光樊龙江卢秀萍王卫媂李永平方敦煌童治军
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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