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Nicotiana tabacum isoflavone reductase-like (NtIRL) gene and application thereof

A technology of common tobacco and isoflavones, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as aggravation, affecting the quality of tobacco production and the income of planting tobacco.

Inactive Publication Date: 2013-04-03
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Moreover, in recent years, tobacco fungal diseases have become more serious, seriously affecting the quality of tobacco production and the income of tobacco planting.

Method used

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  • Nicotiana tabacum isoflavone reductase-like (NtIRL) gene and application thereof
  • Nicotiana tabacum isoflavone reductase-like (NtIRL) gene and application thereof
  • Nicotiana tabacum isoflavone reductase-like (NtIRL) gene and application thereof

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Embodiment Construction

[0073] Specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0074] The medium-nicotine variety of common tobacco used in the examples, Honghua Dajinyuan, and the common tobacco varieties of high-nicotine, Dafuyan and Longli Honghuayan, were provided by Guizhou Tobacco Science Research Institute, and were conventionally planted in the field. A small amount of plant tissue RNA extraction kit and a small amount of gel recovery kit are products of Shanghai Huashun Bioengineering Co., Ltd. GeneRacer Kit is a product of Invitrogen. LA Taq DNA polymerase and pMD18-T vector are products of Bao Biological Engineering (Dalian) Co., Ltd. PCR DIG...

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Abstract

The invention discloses a new member NtIRL2 of a Nicotiana tabacum isoflavone reductase-like (NtIRL) gene family. The sequence of an open reading frame of the NtIRL2 is shown as 79 nucleotide to 1011 nucleotide in SEQ ID NO.10. The invention performs systemic bioinformatic analysis on the members NtIRL1 and NtIRL2 of the NtIRL gene family, sets forth the expression features of the NtIRL1 and NtIRL2 genes in each tissue and organ of Nicotiana tabacum, constructs a plus sense expression vector and an antisense expression vector, and verifies that the NtIRL1 and NtIRL2 genes are coded with activated nicotine synthesis correlated enzymes through genetic transformation. The NtIRL1 and NtIRL2 genes play vital roles in the biosynthesis and metabolic pathway of tobacco nicotine, participate in the fungal disease defense of tobacco plants, can be used for regulating the nicotine content of Nicotiana tabacum and improving the disease resistance, and is beneficial to the gene engineering breeding of high-quality tobacco having low nicotine content and high fungal disease resistance.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, relates to a tobacco gene, and also relates to the application of the gene. Background technique [0002] Tobacco is a plant of the genus Nicotiana in the family Solanaceae of the dicotyledonous plant class Picoflowers. Nicotiana (Nicotiana) has more than 60 species. Among the two cultivated species, common tobacco (also known as safflower tobacco, Nicotiana tabacum) occupies the main area, and yellow flower tobacco (Nicotiana rustica) occupies a small area. Cultivated tobacco can be divided into six types: flue-cured tobacco, sun-cured tobacco, air-dried tobacco, burley tobacco, oriental tobacco and yellow flower tobacco according to the quality characteristics of tobacco leaves, biological characteristics and cultivation methods, among which flue-cured tobacco is the most widely cultivated common tobacco. my country's flue-cured tobacco planting area and total output both rank firs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N15/84C12N1/21A01H5/00
Inventor 陈伟柴友荣李智勇潘文杰雷波王三根
Owner GUIZHOU TOBACCO SCI RES INST
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