Whole genome selection model for predicting nicotine content of tobacco and application thereof
A genome-wide, model selection technology, applied in genomics, proteomics, instruments, etc., can solve the problems of restricting breeding work, low accuracy, and few genetic analysis studies
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[0036] Bayes B, a genome-wide selection model for predicting tobacco nicotine content NIC construction and application
[0037] 1. Experimental materials
[0038] Flue-cured tobacco variety YY3 (high nicotine content, 2.6±0.11 %) and flue-cured tobacco variety K326 (low nicotine content, 1.2±0.13 %) were used to construct a population of recombinant inbred lines (RILs, F7), which contained 300 lines; in addition, In order to construct a predictive model with tobacco generality, a natural population of tobacco, which is different from the population derived from fixed parents, was established, which consisted of 347 different tobacco varieties (lines).
[0039] 2. Data acquisition of nicotine content in leaves of two different types of tobacco populations after curing
[0040] After the test materials become seedlings, they are transplanted to the field. After the field tobacco plants mature, the tobacco leaves are harvested, baked, graded, sample prepared, tested and counted...
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