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Breeding method for natural ganoderma lucidum

A kind of Ganoderma lucidum and wild technology, applied in the field of wild Ganoderma lucidum breeding, can solve the problems of insufficiency and less wild Ganoderma lucidum, and achieve the effects of high yield, strong resistance to miscellaneous bacteria and fast growth.

Inactive Publication Date: 2015-10-07
唐伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because wild Ganoderma lucidum is rare, it is far from satisfying people's needs
At present, there are relatively few reports on breeding methods of wild Ganoderma lucidum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Collection of wild ganoderma: collect wild ganoderma that grows in high mountains at an altitude of 800-1900m. The color is purple-black;

[0036] (2) Preparation of test tube slant medium:

[0037] ① Preparation of mother seed medium: select glutinous rice medium: take 100g of glutinous rice, 25g of glucose, 20g of agar, and 1000mL of water, add water to the glutinous rice, simmer for 20-30 minutes, filter with 2 layers of gauze, get the juice, and then supplement the above juice to 1000mL;

[0038] ②Preparation of test tube slant culture medium: divide the mother seed culture medium obtained in step ① into test tubes, autoclave for 30-50 minutes, and cool down after making test tube slant culture medium;

[0039] (3) Tissue separation: get the fruiting bodies of wild Ganoderma lucidum obtained in step (1), the proterties of the fruiting bodies are early fruiting, normal, strong, large flower shape, immature, rinse with water, cut off the stipe, and then Disinfe...

Embodiment 2

[0045] (1) Collection of wild ganoderma: collect wild ganoderma that grows in high mountains at an altitude of 800-1900m. The color is purple-black;

[0046] (2) Preparation of test tube slant medium:

[0047] ① Preparation of mother seed medium: select potato medium: 200g of peeled potatoes, 20g of glucose, 20g of agar, 1000mL of water, slice potatoes, add 1000mL of water, boil in simmering water for 15-20 minutes, filter with 4 layers of gauze, take the juice, and then put The above juice is supplemented to 1000mL;

[0048] ②Preparation of test tube slant culture medium: divide the mother seed culture medium obtained in step ① into test tubes, autoclave for 30-50 minutes, and cool down after making test tube slant culture medium;

[0049] (3) Tissue separation: get the fruiting bodies of wild Ganoderma lucidum obtained in step (1), the proterties of the fruiting bodies are early fruiting, normal, strong, large flower shape, immature, rinse with water, cut off the stipe, an...

Embodiment 3

[0054] (1) Collection of wild ganoderma: collect wild ganoderma that grows in high mountains at an altitude of 800-1900m. The color is purple-black;

[0055] (2) Preparation of test tube slant medium:

[0056] ① Preparation of mother seed medium: select comprehensive medium: 200g of peeled potatoes, 20g of glucose, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate, vitamin B 1 10mg, agar 20g.

[0057] ②Preparation of test tube slant culture medium: divide the mother seed culture medium obtained in step ① into test tubes, autoclave for 30-50 minutes, and cool down after making test tube slant culture medium;

[0058] (3) Tissue separation: get the fruiting bodies of wild Ganoderma lucidum obtained in step (1), the proterties of the fruiting bodies are early fruiting, normal, strong, large flower shape, immature, rinse with water, cut off the stipe, and then Disinfect with alcohol with a volume fraction of 75%, then rinse with sterile water, dry the water with s...

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Abstract

The invention discloses a breeding method for natural ganoderma lucidum, and belongs to the technical field of edible fungus genetic breeding. The breeding method comprises the following steps of 1 natural ganoderma lucidum collecting, 2 test tube slant culture medium preparing, 3 tissue separating, 4 strain inoculating and cultivating, and 5 inoculating and cultivating. The natural ganoderma lucidum separated by the breeding method is large in mother strain shape, high in yield and high in sundry fungus resisting capacity, the cultivation and expanding problem of the natural ganoderma lucidum can be solved, and the finished product yield is high.

Description

technical field [0001] The invention relates to a breeding method of wild ganoderma lucidum, which belongs to the technical field of genetic selection and breeding of edible fungi. Background technique [0002] Wild Ganoderma lucidum, also known as Xiancao, Ruicao, and Ganoderma lucidum, belongs to the Basidiomycetes Polyporaceae Ganoderma lucidum genus. It is a medicinal fungus that reproduces sexually with spores and uses lignin as the main carbon source nutrient. It has been used since ancient times. It is used as Chinese herbal medicine by our people. Ganoderma lucidum, sweet in taste, flat in nature, home to the heart, lung, liver, and kidney meridian, has the effects of nourishing qi and nourishing blood, nourishing the heart and calming the nerves, relieving cough and calming cough, and mainly treating diseases such as fatigue, cough, asthma, insomnia, indigestion, and malignant tumors . [0003] The mycelium, fruiting bodies and spores of Ganoderma lucidum can be u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14C05G3/00
CPCA01G18/00C05D3/00C05F5/002C05F11/00C05G3/00C12N1/14
Inventor 唐伟
Owner 唐伟
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