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Supercritical fluid extract technology of ganoderma volatile oil, ganodenic acid and ganoderma polysaccharide

A technology of supercritical fluid, Ganoderma lucidum polysaccharide, applied in the fields of plant raw materials, organic chemistry, antitumor drugs, etc., can solve the problems of poor product quality, low extraction efficiency, low recovery rate of active ingredients, etc. High acquisition rate and the effect of protecting biological activity

Inactive Publication Date: 2010-03-10
HUNAN HEPING BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has low extraction efficiency, low active ingredient recovery and poor product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Take the red Ganoderma lucidum mycelium fermented in liquid, dry it in a vacuum box at 60°C for 12 hours, pulverize it in a plant grinder, pass through a 40-mesh sieve, and obtain Ganoderma lucidum mycelium powder; (2) Weigh 1kg of Ganoderma lucidum mycelium Body powder, added to 1 L of supercritical CO 2 In the extraction kettle of the fluid extractor, set the extraction pressure to 20Mpa, the extraction temperature to 40°C, and CO 2 The flow rate is 100L / min, and the extraction time is 2h; 60g of bright yellow and transparent Ganoderma lucidum volatile oil is obtained from the separation kettle; (3) Keep the Ganoderma lucidum powder in the extraction kettle unchanged, adjust the extraction pressure to 30Mpa, and the extraction temperature to 45°C. Use distilled water as an entrainer, extract for 2 hours, and use primary separation to separate to obtain an aqueous solution containing ganoderma acid and ganoderma polysaccharides. Add 4 times the volume of 95% (V / V) ...

Embodiment 2

[0018] (1) Take artificially cultivated Ganoderma lucidum fruiting bodies, dry them in a vacuum box at 55°C for 15 hours, pulverize them in a plant pulverizer, and pass through a 60-mesh sieve to obtain Ganoderma lucidum fruiting body powder; (2) Weigh 10kg of Ganoderma lucidum fruiting body powder , added to 10L of supercritical CO 2 In the extraction kettle of the fluid extractor, set the extraction pressure to 18Mpa, the extraction temperature to 42°C, and CO 2 The flow rate is 1100L / min, and the extraction time is 110min; 650g of bright yellow and transparent Ganoderma lucidum volatile oil is obtained from the separation kettle; (3) keep the Ganoderma lucidum fruiting body bacteria powder in the extraction kettle unchanged, adjust the extraction pressure to 29Mpa, and the extraction temperature to 46 ℃, use distilled water as the entrainer instead, extract for 130min, and separate by primary separation method to obtain an aqueous solution containing ganoderma acid and gano...

Embodiment 3

[0020] (1) Take the red Ganoderma lucidum mycelium fermented in liquid, dry it in a vacuum box at 65°C for 10 hours, pulverize it in a plant grinder, pass through a 40-mesh sieve, and obtain Ganoderma lucidum mycelium powder; (2) Weigh 1kg of Ganoderma lucidum mycelium Body powder, added to 1 L of supercritical CO 2 In the extraction kettle of the fluid extractor, set the extraction pressure to 21Mpa, the extraction temperature to 39°C, and CO 2 The flow rate is 98L / min, and the extraction time is 130min; 61g of bright yellow and transparent Ganoderma lucidum volatile oil is obtained from the separation kettle; (3) keep the Ganoderma lucidum powder in the extraction kettle unchanged, adjust the extraction pressure to 31Mpa, and the extraction temperature to 44°C. Use distilled water as an entrainer, extract for 130 minutes, and use primary separation to separate to obtain an aqueous solution containing ganoderma acid and ganoderma polysaccharides. Add 4 times the volume of 95%...

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Abstract

The invention discloses a supercritical fluid extract technology of ganoderma volatile oil, ganodenic acid and ganoderma polysaccharide, which comprises the following steps: (1) drying and crushing ganoderma mycelium or ganoderma sporocarp; (2) adding ganoderma microbe powder into the extraction cauldron of a supercritical CO2 fluid extracting machine, setting the extracting pressure to be 15-25 MPa and the temperature to be 38-42 DEG C, leading 95-105L / min CO2 fluid, extracting for 1.5-2.5h, and obtaining the ganoderma volatile oil form a separation kettle; and (3) adjusting the extracting pressure to be 28-32 MPa and the extracting temperature to be 44-47 DEG C, taking distilled water as the entrainer, extracting for 1.5-2.5h, obtaining the water solution containing the ganodenic acid and ganoderma polysaccharide, adding 3.5-4.5 times of volume of 95% (V / V) ethanol in the solution to generate flocculent precipitate, placing for 3-6h, centrifugalizing, separating, freezing and dryingthe precipitate, thus obtaining the ganoderma polysaccharide product; decompression-concentrating the centrifugalized liquid supernatant to obtain the extract containing the ganodenic acid. The supercritical fluid extract technology has simple operation, small organic solvent usage, high extraction efficiency, high recovery rate of active ingredients, good product quality and low production cost.

Description

technical field [0001] The invention relates to a preparation process of ganoderma volatile oil, ganoderma acid and ganoderma polysaccharide, in particular to a supercritical fluid extraction process of ganoderma volatile oil, ganoderma acid and ganoderma polysaccharide. Background technique [0002] Ganoderma lucidum has a long history of clinical use as a precious Chinese herbal medicine. [0003] Ganoderma lucidum is rich in protein, vitamins, various physiologically active substances (such as polymer polysaccharides), natural organic germanium, organic acids, triterpenoids, etc. It has very important value for maintaining human health and is also a worldwide health food. [0004] With the advancement of science and technology and the development of modern biotechnology, various health products and beauty products of Ganoderma lucidum with food and therapeutic effects, as well as special food medicines for the treatment of geriatric diseases, chronic diseases, certain nut...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P37/04A61P35/00C08B37/00C07J9/00
CPCY02P20/54
Inventor 喻桃生夏志兰
Owner HUNAN HEPING BIOLOGICAL TECH CO LTD
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