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Method for preparing cordyceps militaris

A technology of Cordyceps militaris and strains, applied in botany equipment and methods, fertilizer mixture, horticulture, etc., can solve the hidden dangers of Cordyceps militaris products, pollution of human body and environment, streptomycin residue and other problems, and achieve improved resistance to miscellaneous bacteria Ability, promotion of robust growth, effect of reducing dosage

Inactive Publication Date: 2015-07-15
李天兰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in the propagation stage of cultivating Cordyceps militaris strains, the addition of foreign aid antibiotics in the culture medium is effective for the prevention and treatment of the proliferation of Cordyceps militaris strains, but the formation of Cordyceps militaris has the characteristics of green and streptomyces Toxin residues are easy to pollute the human body and the environment, causing a great safety hazard for Cordyceps militaris products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Material preparation process:

[0033] (1) Strain preparation: Cordyceps militaris strains are preferred, cultured in Erlenmeyer flask liquid medium, the formula is 2.55% potatoes, 1.2% magnesium sulfate, 1% potassium dihydrogen phosphate, 0.45% dipotassium hydrogen phosphate, vitamin B1 0.008 %, vitamin B6 0.014%, this formula is an improved PDA medium, after it is prepared, it is sterilized at a pressure of 0.11 MPa and 122°C for 25 minutes. Inoculate the species of Cordyceps militaris into the culture medium of the Erlenmeyer flask for culture, the culture conditions are shaker culture, the rotation speed is 160r / min, the temperature is 20°C, and the mycelium balls that appear in the Erlenmeyer flask after about 5 days of dark culture are evenly suspended and can be used. .

[0034] (2) Culture medium preparation: use rice culture medium, the nutrient composition of the culture medium is 5% of soybean, 9.5% of coconut meat powder, 0.08% of camellia pollen, 7% of gl...

Embodiment 2

[0041] (1) Strain preparation: Cordyceps militaris strains are preferred, cultured in Erlenmeyer flask liquid medium, the formula is 3.2% potatoes, 0.6% magnesium sulfate, 1.15% potassium dihydrogen phosphate, 0.3% dipotassium hydrogen phosphate, vitamin B1 0.010 %, vitamin B6 0.010%, this formula is an improved PDA medium, after it is prepared, it is sterilized at a pressure of 0.11 MPa and 122°C for 25 minutes. The species of Cordyceps militaris was inoculated into the culture medium of the Erlenmeyer flask, and the culture conditions were shaker culture, the rotation speed was 155r / min, the temperature was 20°C, and the mycelium balls that appeared in the Erlenmeyer flask after 6 days of dark culture were evenly suspended and then used.

[0042] (2) Culture medium preparation: choose rice culture medium, the nutrient composition of the culture medium is 7% of soybean, 7.2% of coconut meat powder, 0.15% of pumpkin pollen, 5.5% of glucose, 0.75% of magnesium sulfate, and 0.008...

Embodiment 3

[0049] (1) Strain preparation: Cordyceps militaris strains are preferred, cultured in Erlenmeyer flask liquid medium, the formula is 4% potatoes, 1.0% magnesium sulfate, 1.2% potassium dihydrogen phosphate, 0.25% dipotassium hydrogen phosphate, vitamin B1 0.015 %, vitamin B6 0.006%, this formula is an improved PDA medium, after it is prepared, it is sterilized at a pressure of 0.11 MPa and 122°C for 25 minutes. The species of Cordyceps militaris was inoculated into the culture medium of the Erlenmeyer flask, and the culture conditions were shaker culture, the rotation speed was 160r / min, the temperature was 20°C, and the mycelium balls that appeared in the Erlenmeyer flask after 5 days of dark culture were evenly suspended and then used.

[0050] (2) Culture medium preparation: choose rice culture medium, the nutrient composition of the culture medium is calculated as the mixture of soybean and black soybean 10% by mass percentage, coconut meat powder 5.5%, orange pollen 0.10%,...

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Abstract

The invention provides a method for preparing cordyceps militaris, comprising the following steps of preparing strains, preparing a cultivation substrate, cultivating a strain-receiving substrate, and harvesting and drying after the strains are cultivated maturely, wherein the step of preparing the strains is performed by means of cultivating cordyceps militaris strains in the substrate, cultivating by utilization of a rotary swing bed, and cultivating at a constant temperature for a certain time till mycelium pellets are formed and uniformly float; in the step of preparing the cultivation substrate, the cultivation substrate is composed of a substrate body and a culture solution; the ingredients of the culture solution comprise beans, coconut powder, pollen, glucose, magnesium sulfate, vitamin B1, vitamin B6 and the rest of alkalescent water; the material-to-liquid ratio of the substrate body to the culture solution is 1:1.7-1.9; the moisture content of a dried product is not greater than 8%. The cordyceps militaris prepared according to the method for preparing the cordyceps militaris has the advantages that the color is golden, conidial powder is contained, the contents of cordycepin, cordyceps polysaccharide and superoxide dismutase (SOD) are high, the formula and method provided by the invention are simple and are easily obtained, the ingredients which are used for preparing the culture solution are simple and are easily obtained, and the cost is low.

Description

technical field [0001] The invention belongs to the field of food preparation, and relates to a cultivation and production process of Cordyceps militaris, in particular to a preparation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris is a type species of Ascomycota, Ergot, Ergotaceae, and Cordyceps. The scientific name is Cordyceps militaris, also known as Cordyceps militaris, etc. Its natural quantity is very small. The current research focuses on the cultivation of domesticated high-quality strains and expanded cultivation in factories. The nutritional value of its dried products is higher than that of wild ones. [0003] Chemical constituents of Cordyceps militaris: N6-[β-(acetamidoformyl)oxyethyl]adenosine; Cordyceps cyclopeptide A; nucleosides; ergosterol peroxide. Cordyceps militaris - Pharmacological action Modern scientific evidence Cordyceps militaris not only has special nutritional value, but also has obvious medicinal value. Con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 李天兰
Owner 李天兰
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