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Bacillus licheniformis and application of bacillus licheniformis

A technology of bacillus licheniformis and bacteria slime, applied in the direction of application, bacteria, microorganisms, etc., to achieve the effects of improving feed conversion rate, improving production performance, and strong stress resistance

Active Publication Date: 2013-01-16
北京龙科方舟生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research is mostly focused on the development of products and application effects, and the selection of strains with excellent performance according to the specific requirements of probiotics, and few reports on the biological characteristics of strains

Method used

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  • Bacillus licheniformis and application of bacillus licheniformis
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  • Bacillus licheniformis and application of bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1. Isolation and Identification of Bacillus Licheniformis C30-2

[0070] 1. Isolation and screening of strain C30-2

[0071] 1. Isolation and cultivation of bacterial strains

[0072] Take 1g of the farmland soil sample in Beijing, China, and put it in a test tube filled with 9mL of sterile saline, and vortex to mix it evenly, which is a 1:10 dilution, and then take the dilution for ten-fold incremental dilution, and then choose 3 suitable gradients 1mL each of the diluted solution was spread on the MRS agar medium and M17 agar medium containing 10ppm cycloheximide, cultured anaerobically at 37°C for 48-72h, observed and recorded the colony shape, and picked a characteristic single colony with good growth on the MRS Streak separation and purification in broth medium. Observe whether the broth becomes turbid, and store the turbid bacterial suspension in a refrigerator at 4°C, and then carry out the following ultraviolet mutagenesis screening experiment.

[007...

Embodiment 2

[0096] Example 2, Stress Resistance Detection of Bacillus Licheniformis C30-2 CGMCC No.5350

[0097] 1. Heat resistance test

[0098] Bacillus Licheniformis (Bacillus Licheniformis) C30-2 CGMCC No.5350 was inoculated at 2% (v / v) (3.75×10 10 cfu / ml) were inoculated into MRS broth medium, a total of six copies were treated in a water bath at 85°C for 10min, 15min, 20min, 30min, 40min, and 60min, and then cultured on an MRS agar plate at a constant temperature of 37°C for 24h. The plate pouring method was used to determine the number of viable bacteria.

[0099] The results are shown in Table 2. The survival rate of Bacillus Licheniformis C30-2 CGMCC No.5350 was 93.33% after treatment at 85°C for 10 minutes, and 24% after treatment for 15 minutes. It proved that the strain has better heat resistance. Generally, the pelleting temperature of piglet feed is between 70°C and 85°C, and the low survival rate after high temperature resistance is also one of the main limiting factors ...

Embodiment 3

[0114] Embodiment 3, enzyme activity detection and antibacterial test

[0115] Staphylococcus aureus (Staphylococcus aureus CVCC1882, National Veterinary Microbiology Collection Center), Escherichia coli (E.coli) K88 (Escherichia coli) (CMCC44742, China Medical Bacteria Collection Management Center) involved in this example, Escherichia coli (E.coli) O157 (purchased from China Veterinary Drug Administration), Salmonella typhi (National Veterinary Microbiology Collection Center), Bacillus pyogenes (National Veterinary Microbiology Collection Center), Meridian arc Vibrio metschnikovi (National Veterinary Microorganism Collection), Clostridium welchii (National Veterinary Microbiology Collection), Micrococcus luteus (National Veterinary Microbiology Collection), Streptococcus pneumoniae (Streptococcus pneumoniae, National Veterinary Microbial Culture Collection Center), Bacillus megaterium (National Veterinary Microbiological Culture Collection Center), Bacillus pumilus (National...

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Abstract

The invention discloses a bacillus licheniformis and application of the bacillus licheniformis. The bacillus licheniformis provided by the invention is concretely bacillus licheniformis C30-2 with the preservation number being CGMCC (China General Microbiological Center Culture Collection Center) No.5350 in CGMCC. Experiments prove that the bacillus licheniformis C30-2 CGMCC No.5350 provided by the invention has strong stress resistance and strong sundry fungus resistance capability. The bacillus licheniformis C30-2 CGMCC No.5350 is emulsified for preparing the bacillus licheniformis preparation, the bacillus licheniformis preparation can be used as additives used for preparing animal feed and can replace antibiotics in animal daily ration for regulating the microecological balance in the animal intestinal tracts, so the effect of enhancing the nonspecific immunity function for realizing the effect of preventing the diseases is realized, and meanwhile, nutrition factors can also be provided for promoting the digestion absorption of nutrition substances, reducing the diarrhea, promoting the animal growth and improving the animal feed conversion rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a bacillus licheniformis and its application. Background technique [0002] As people attach great importance to health and ecological environment, non-toxic, non-residual and non-resistance green feed additives have become a research hotspot. Probiotics are live bacterial food or feed additives that are beneficial to the balance of host intestinal microorganisms. They are required to resist the environment in the digestive tract, colonize on the surface of the digestive tract, produce useful enzymes and organic acids and other metabolites, and maintain activity during processing and storage. Wait. There are many kinds of probiotics as green feed additives. US FDA (1989) believes that there are 42 kinds of safe microbial strains, and 16 kinds are announced in the 658 announcement of the Ministry of Agriculture, and Bacillus clicheniformis is one of them. [0003] Bacillus lichenifor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A23K1/00C12R1/10
Inventor 谯仕彦丁修良王劲松宋青龙张海燕
Owner 北京龙科方舟生物工程技术有限公司
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