The present invention discloses a tremella cultivation method, characterized in that corn cores and bean cakes serve as the main materials, and a mushroom tang immersion material is added for stacking fermentation. The leach solution of the mushroom tang rejuvenates ear bed hypha, and the biological efficiency reach 14.0% (dried). The tremella cultivation method comprises the steps of material preparation, fermentation, bed making, seeding, spawn running and ear generating management. The tremella cultivation method has the advantages as follows: firstly, the mushroom tang immersion material and cultivation materials are mixed for stacking fermentation, the breeding of profitable microbe is prompted, and the catalysis decomposition of the cultivation material is accelerated; secondly, the fermented cultivation material has high activity profitable fungus and active metabolic product thereof, the growth of tremella hyphae and ash hyphae is prompted, the growth trend of the hyphae is lively, and the anti-mixed bacterium ability is high; thirdly, after the first round of picking up of the tremella, the effect of rejuvenating the mushroom tang leach solution is good, and the conversion rate of the second round of the tremella is high; fourthly, resources such as manpower, coal, carbon are saved, environmental pollution is reduced, according with the concept of low-carbon environmental protection.