Method for direct seeding and seedling raising of pleione bulbocodioides by using symbiotic bacteria

A technology of solitary orchid and symbiotic bacteria, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of slow growth of sterile seedlings, cumbersome steps, and limitations of symbiotic cultivation, and achieve rapid seed germination and seedling formation , high survival rate in the field, suitable for popularization and application

Pending Publication Date: 2022-08-09
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the exploration of the nutritional conditions required by different species, many orchids have been able to obtain seedlings through aseptic sowing, but the production cost of sterile germination seedlings is high and the steps are cumbersome, such as seed germination, protocorm differentiation, and seedling subculture. Different media are usually required for the stage of rooting and strong seedlings, and the aseptic seedlings grow slowly after being released from the bottle and are susceptible to disease.
The artificial cultivation of garlic orchids is still in

Method used

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  • Method for direct seeding and seedling raising of pleione bulbocodioides by using symbiotic bacteria
  • Method for direct seeding and seedling raising of pleione bulbocodioides by using symbiotic bacteria
  • Method for direct seeding and seedling raising of pleione bulbocodioides by using symbiotic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Verify the effectiveness of Indian pear -shaped spores to promote the symbiotic germination of lonely garlic seeds

[0038] (1) Revolution and identification of the strain

[0039] Public bacteria library and laboratory usually use glass test tube and slope medium at low temperature to preserve bacteria at low temperatures. Therefore, the newly obtained bacteria are usually in a lower vitality, and they need to be transferred to the cultivatt to activate before use. In order to confirm the genetic information of the strain, the strain DNA needs to be extracted, the NRDNAITS interval of the strain is required, and the order of the NRDNAITS interval is compared with the corresponding fungal sequence in the Genbank database.

[0040] A. Preparation of PDA medium: Cook the potato after peeling, cooked with 200g of soft potatoes, takes filtrate, add glucose 20g, agar 16g, and set to 1 liter with distilled water.

[0041] B. Deduct with triangular bottle and sterilize i...

Embodiment 2

[0052] Example 2 uses Indian pear -shaped spores to perform live -broadcast seedlings

[0053] (1) Preparation of pear -shaped spore suspension: Step Step Step 1 Step 1 (3) A ~ B.

[0054] (2) Coconut bran live broadcast of seedlings

[0055] A. Mix coconut bran and gravel in 1: 1, put it in a cloth bag, and sterilize in a high -pressure sterilization cooker. After cooling, place it in a flat plastic box, and the top of the plastic box is left with an air pores.

[0056] B. Take the mixture of Indian pearbacteria suspension through the mixed matrix of the gravel and coconut bran. Take the solo garlic seeds (see Figure 4 ) Swap to the surface of the matrix and spray the seed surface with a spray bottle.

[0057] C. Cover the transparent box cover and place the plastic box in an artificial climate box. It is cultivated at 12h / dark 12h, light strong 2500lx, and 25 ° C.

[0058] D. Regularly spray sterile water to keep the surface of the matrix humid. After 10-15 days, most of the see...

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PUM

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Abstract

The invention provides a method for direct sowing and seedling raising of pleione bulbocodioides by utilizing symbiotic bacteria, which comprises the following steps: mixing piriformospora indica into a culture medium, sowing pleione bulbocodioides seeds, and performing symbiotic culture to obtain symbiotic pleione bulbocodioides seedlings. The method is simple and convenient to operate, the symbiotic bacteria and pleione bulbocodioides seeds are directly sown for seedling raising for the first time, after 10-15 days, most of the seeds appear green, after 30 days, a large number of protocorms appear native meristem, after 45 days, the protocorms grow first leaves, after 2 months, seedlings form 2-3 leaves and grow roots, and the seedlings can be transplanted to a nursery garden.

Description

Technical field [0001] The present invention belongs to the field of Lanke plant cultivation technology, which involves a method that uses symbiotic bacteria for livelihood seedlings. Background technique [0002] Pleione Bulbocodioides is a unique flower resource in my country. It is listed as a national second -level protection plant and is mostly distributed in the southwest mountain forest. In the wild, this type can be attached to the branches of a variety of trees, with gorgeous colors, long flowering period, and high ornamental value. The species of this species is included in the pills of the pills as "Yami Mushrooms" or "Ice Hockey", which has the effects of treating bloated and poison, sputum nucleus, lymph node tuberculosis and snake bugs. [0003] Lanke plants can reproduce through natural division and organizational training, but the natural division of breeding coefficients is low, and the organizational training technology is not yet mature. Based on the exploratio...

Claims

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Application Information

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IPC IPC(8): A01G22/63A01G7/06A01G24/25A01G24/12
CPCA01G22/63A01G7/06A01G24/25A01G24/12Y02P60/20
Inventor 秦姣张石宝张伟胡中会杨玉凡
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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