Method for improving resistance of plants to bensulfuron methyl serving as herbicide by using Piriformospora indica

A technology of piriformis and bensulfuron-methyl in India, which is applied in the field of crop planting, can solve the problems of crop phytotoxicity, achieve the effects of reducing phytotoxicity, improving resistance and ensuring safety

Active Publication Date: 2018-04-20
ZHEJIANG UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a method for improving the resistance of crops to the herbicide

Method used

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  • Method for improving resistance of plants to bensulfuron methyl serving as herbicide by using Piriformospora indica
  • Method for improving resistance of plants to bensulfuron methyl serving as herbicide by using Piriformospora indica
  • Method for improving resistance of plants to bensulfuron methyl serving as herbicide by using Piriformospora indica

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited thereto.

[0041] Materials used in the present invention: Piriformospora indica (Piriformospora indica) is donated by Professor Lou Binggan of Zhejiang University; Zhongzheyou No. 1 rice seed is provided by Hangzhou Seed Management Station, and 10% bensulfuron-methyl wettable powder is purchased from Anhui Huaxing Chemical Co., Ltd.

[0042] Determination of the susceptibility test of Piriformis indica to bensulfuron-methyl on 1 plate

[0043] 1.1 Cultivation of Piriformospora indica

[0044] Inoculate the mycelia of Pyromorpha indica on the PDA medium plate, and then place the culture dish in a 28°C, 24h dark constant temperature incubator for cultivation, and set aside.

[0045] 1.2 Preparation of media containing different concentrations of bensulfuron-methyl

[0046]Cool the melted 1L PDA medium to about 45°C, pipette an appropria...

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Abstract

The invention discloses an application of Piriformospora indica to improvement of the resistance of plants to bensulfuron methyl serving as a herbicide and belongs to the technical field of crop planting. The Piriformospora indica is not inhibited by bensulfuron methyl, and the established Piriformospora indica-crop co-culture system is capable of remarkably improving the resistance of crops to bensulfuron methyl and reducing phytotoxicity; and after the Piriformospora indica serving as a beneficial endophytic fungus is colonized into the crops, no side effects are generated, and meanwhile, the environment safety is guaranteed. The crops are rice, rapes, sunflowers, tobaccos, wheat and maize. The invention further provides a method for improving the resistance of rice to bensulfuron methylserving as a herbicide. The method comprises the steps: co-culturing rice seedlings in a rice culture solution containing Piriformospora indica to obtain rice seedlings in which the Piriformospora indica is colonized, and then, transplanting the rice seedlings in which the Piriformospora indica is colonized to a farmland, and culturing the rice seedlings until harvesting rice.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for improving the resistance of crops to the herbicide bensulfuron-methyl by using Pyromorpha indica. Background technique [0002] Rice is one of the most important food crops in the world. Weeds in rice fields are the main factor causing rice production reduction. The application of herbicides plays an important role in ensuring grain production and stable production. It is currently the most common weeding method in the world. Way. [0003] Bensulfuron-methyl (BSM; trade name: Nongdex) is a selective systemic conduction type sulfonylurea herbicide developed by DuPont Company of the United States. Safe paddy sulfonylurea herbicides are used all over the world, among which China, Japan, South Korea and Southeast Asia have the largest application areas. Especially in my country, BSM is the preferred herbicide for rice fields in recent years, mainly because my cou...

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Application Information

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IPC IPC(8): A01G22/22A01G31/00C12N1/14C12R1/645
CPCA01G31/00C12N1/14
Inventor 吕梦婷周伟军王尖李兰许玲白全江宋文坚朱金文
Owner ZHEJIANG UNIV
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