The invention discloses a guide pipe adjusting type radio-frequency cable take-up device. The guide pipe adjusting type radio-frequency cable take-up device comprises a rack, a pay-off reel and a take-up reel are arranged on the rack, a fixing plate is arranged on the inner bottom of the rack, side plates are arranged on the fixing plate, and a carrying plate is arranged between the ends of the side plates. A driving motor is arranged on the carrying plate, a driving shaft is arranged on the front portion of the driving motor, one end of the driving shaft is connected with the driving motor, and the other end of the driving shaft is sleeved with a driving wheel. The driving wheel is engaged with the take-up reel. A moving frame is arranged in the rack, a guide shaft is arranged in the moving frame, the peripheral surface of the guide shaft is sleeved with sliding pipes, guide pipes are arranged on the sliding pipes, cyclophons are sleeved with the guide pipes, and traction wheels are arranged at the ends of the cyclophons. According to the guide pipe adjusting type radio-frequency cable take-up device, the moving frame is convenient to move and adjust, the traction wheels can be moved and adjusted, and thus a radio-frequency cable can be wound in the axial position of the peripheral surface of the take-up reel and can be wound on the whole outer surface of the take-up reel.