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Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos

A technology of Pyromorpha spp. and bacteriocin, which is applied in the field of plant disease prevention and control, can solve the problem that biocontrol bacteria and pesticides cannot be used together, and improve the effect of prevention and control

Active Publication Date: 2016-07-27
ZHEJIANG UNIV
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Problems solved by technology

[0008] The invention provides a method for jointly preventing and treating tobacco bacterial wilt by using Piroformis sp. Defects that anti-bacteria and pesticides cannot be used together

Method used

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  • Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos
  • Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos
  • Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos

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Embodiment Construction

[0041] The present invention will be further described below in conjunction with specific examples.

[0042]Materials used in the present invention: Indian piriformis (P.india) was donated by Professor Ajit Verma of the University of Edinburgh in India; Ralstonia solanacearum TB3 (RS-TB3) was donated by researcher Feng Jie of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences; China Tobacco 100 seeds are provided by Zhengzhou Tobacco Research Institute;

[0043] 3% Zhongshengmycin was purchased from Hunan Marine Biological Engineering Co., Ltd.

[0044] 1 Plate Determination of Bacteriostatic Effect on Ralstonia solanacearum

[0045] 1.1 Bacterial suspension preparation

[0046] The highly pathogenic strain RS-TB3 preserved in sterile water was streaked on the TTC plate, and after 48 hours of culture, a single colony was picked and streaked on the NA plate (NA medium formula: beef extract 3g, peptone 5g, glucose 2.5 g, agar 20g, distilled water 1000...

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Abstract

The invention discloses a method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos. The method includes the following steps that 1, tobacco seeds are sowed in a seedling raising substrate mixed with piriformospora indica hyphae, and seedlings are transplanted to fields after seedling development; 2, before transplanting, holes for tobacco seedling transplanting are irrigated with a Zhongshengmycin solution; root-irrigation treatment is conducted on tobacco plants by using the Zhongshengmycin solution. The piriformospora indica which is adopted by the method and is not inhibited by the Zhongshengmycin can combine with the fungicide Zhongshengmycin for usage and is applied to prevention control of the bacterial wilt of the tobaccos. Compared with the prevention and control effect of the separately used piriformospora indica or the Zhongshengmycin, the jointly used piriformospora indica and Zhongshengmycin can play a synergic effect and remarkably improve the prevention and control effect on the bacterial wilt of the tobaccos.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to a method for jointly preventing and treating tobacco bacterial wilt by utilizing Pyromorpha indica and Zhongshengmycin. Background technique [0002] Tobacco bacterial wilt is an important soil-borne bacterial disease on tobacco. At present, most of the prevention and control methods focus on chemical control. The use is also easy to cause bacteria to develop drug resistance, which brings difficulties to the continuous prevention and control of diseases. [0003] In recent years, more and more people pay attention to the biological control of plant diseases. Biological control is to use beneficial microorganisms to produce various adverse effects on pathogenic bacteria (antibacterial, bacteriolytic, competition, hyperparasitic) so as to reduce the number of pathogens to weaken their pathogenic bacteria. At the same time, beneficial microorganisms used for biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00A01G7/06
CPCA01G7/06A01G22/00A01G24/00
Inventor 王洪凯曹以衬楼兵干苏珍珠林福呈
Owner ZHEJIANG UNIV
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