Method for lowering content of chromium in tobacco

A technology of chromium content and tobacco leaves, which is applied in the field of utilization of microbial resources and quality and safety of agricultural products, can solve the problems that arbuscular mycorrhizal fungi cannot grow and is unfavorable for large-scale promotion and application, and achieves no secondary pollution, convenient application, and low cost. low effect

Active Publication Date: 2014-06-25
CHINA TOBACCO GUIZHOU IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, arbuscular mycorrhizal fungi cannot grow on synthetic media,

Method used

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  • Method for lowering content of chromium in tobacco
  • Method for lowering content of chromium in tobacco
  • Method for lowering content of chromium in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Using Pyromorpha indica to reduce heavy metal chromium residues in hydroponic tobacco includes the following steps:

[0040] Activation of strains: Inoculate the Pyromorpha indica strains stored in the refrigerator on Kafer medium (KM) plates, and culture them in a 28°C incubator in the dark for 7 days. Use a hole punch with a diameter of 5 mm to punch holes at the edge of the activated Pyrocytosporum indica colony.

[0041] Bacteria culture: prepare liquid Kafer medium and liquid Kafer medium containing nanomaterial ZnO (30nm) 0.01g / L (that is, replace ZnSO4 with nanomaterial ZnO with a particle size of 30nm), and pack in 250mL triangular conical flasks , 150mL culture medium in each bottle, sterilized for later use. Under sterile conditions, add the mycelia blocks of Pyromorpha indica into the Erlenmeyer flask containing the above medium, 6 pieces of mycelium blocks per bottle; place in a constant temperature shaker at 120rpm, 28°C and avoid light for 5- 7 days.

...

Embodiment 2

[0061] In Example 1, the mycelia block of Pyriformis indica was changed into Pyromorpha indica spore liquid, inoculated in the liquid Kafer medium containing 10mg / LZnO, and cultured in a constant temperature shaker at 120rpm and 25°C in the dark for 7 days, and the rest Equal to Example 1, the chromium content in the final hydroponic, potted and field cultivated tobacco leaves decreased by 54.2%, 41.2%, and 51.4%, respectively.

Embodiment 3

[0063] In Example 1, the concentration of Pyromorpha indica mycelia liquid was changed to 2g / L, and the rest were equal to Example 1. Finally, the content of chromium in hydroponic, potted and field cultivated tobacco leaves decreased by 50.2%, 41.0%, and 50.9% respectively.

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Abstract

The invention discloses a method for lowering the content of chromium in tobacco. According to the method for lowering the content of chromium in the tobacco, piriformospora indica is grown on the root of the tobacco in a parasitic manner so as to reduce heavy metal chromium residuals in the tobacco. The method for lowering the content of chromium in the tobacco specifically comprises the steps that (1) ZnSO4 7 H2O in an original Kafer culture medium is replaced by nanometer material zinc oxide, and 10 mg of nanometer material zinc oxide is added to each liter of culture medium; (2) activated Piriformospora indica is inoculated to the Kafer culture medium obtained in the step (1) to be cultured, then mycelia are collected, piriformospora indica hypha liquid is prepared, the prepared piriformospora indica hypha liquid is then poured to the root of a tobacco seedling, and root-irrigation treatment is conducted, so that the piriformospora indica is colonized on the root of the tobacco seedling; (3) after the piriformospora indica is colonized on the root of the tobacco seedling, the tobacco seedling is transplanted to a land, and then conventional water and fertilizer management is conducted to make the tobacco seedling grow normally. According to the method for lowering the content of chromium in the tobacco, the growth condition of the tobacco can be improved, the amount of heavy metal chromium which transfers from the root of the tobacco to the overground part of the tobacco is reduced, the heavy metal chromium residuals in the tobacco are reduced, the method is easy to master, the cost is low, the method is easy and practical, environmental friendliness is achieved, and secondary pollution can not be caused.

Description

technical field [0001] The invention relates to the technical field of utilization of microbial resources and quality and safety of agricultural products, in particular to a method for reducing the chromium content of tobacco leaves. Background technique [0002] With the extensive use of chemical fertilizers and pesticides and the rapid development of industry, environmental pollution has become increasingly serious. Chromium is a toxic heavy metal and one of the major environmental pollutants. Tobacco is one of the important economic crops in my country. The planting area, flue-cured tobacco output and production volume of China rank first in the world. In recent years, there have been reports of quality and safety issues such as excessive heavy metals in tobacco, among which cadmium, chromium, and lead have a more serious impact on tobacco growth and quality. Chromium pollution in soil causes tobacco growth retardation and development lag, reduces the content of total n...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06C05G3/00
Inventor 聂长春刘剑刘孜王玉平万里兴万强楼兵干惠非琼刘晓曦高其康林福呈
Owner CHINA TOBACCO GUIZHOU IND
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