Method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii

A technique of tissue culture seedlings and tissue culture, applied in the field of gerbera seedlings, can solve the problems of poor root system development of transplanted seedlings of gerbera, achieve the practical application value and economic value of large-scale production, improve quality and commodity value, and be sustainable highly operable effect

Active Publication Date: 2017-09-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for promoting the rooting of gerbera tissue culture transplanted seedlings, which can effectively s

Method used

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  • Method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii
  • Method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii
  • Method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Preparation of Piroformis indica Fermented Liquid

[0028] Activate the Pyromorpha indica strain, then pick the aerial hyphae at the edge of the colony and inoculate it on a new PDA solid medium. Place it in a dark incubator at 28°C for 5 days, pick mycelium into a 250ml Erlenmeyer flask filled with 100 ml of sterilized PDA liquid medium, and place it on a shaker at a temperature of 28°C and a speed of 120 r / min Cultivate within 3 days, collect the filtrate after filtering through a single layer of gauze, and dilute with water to a spore concentration of 2×10 7 Individuals / ml to obtain the fermentation liquid of Pyromorpha indica.

[0029] 2. Transplanting of Gerbera chrysanthemum tissue culture seedlings

[0030] Gerbera chrysanthemum tissue culture seedlings with a height of 3-4 cm were selected and cultured in the rooting medium for 30 days, which had been continuously subcultured for 5 generations. After opening the cover and hardening the seedlings for 2 days...

Embodiment 2

[0035] 1. Preparation of Piroformis indica Fermented Liquid

[0036] Activate the Pyromorpha indica strain, then pick the aerial hyphae at the edge of the colony and inoculate it on a new PDA solid medium. Place it in a dark incubator at 28°C for 5 days, pick mycelium into a 250ml Erlenmeyer flask filled with 100 ml of sterilized PDA liquid medium, and place it on a shaker at a temperature of 28°C and a speed of 120 r / min Cultivate within 3 days, collect the filtrate after filtering through a single layer of gauze, and dilute with water to a spore concentration of 2×10 7 Individuals / ml to obtain the fermentation liquid of Piriformis indica.

[0037] 2. Transplanting of Gerbera chrysanthemum tissue culture seedlings

[0038] Gerbera chrysanthemum tissue culture seedlings with a height of 3-4 cm were selected and cultured in the rooting medium for 30 days, which had been continuously subcultured for 10 generations. After opening the cover and hardening the seedlings for 2 day...

Embodiment 3

[0043] 1. Preparation of Pyromorpha indica fermentation broth

[0044]Activate the Pyromorpha indica strain, then pick the aerial hyphae at the edge of the colony and inoculate it on a new PDA solid medium. Place it in a dark incubator at 28°C for 5 days, pick mycelium into a 250ml Erlenmeyer flask filled with 100 ml of sterilized PDA liquid medium, and place it on a shaker at a temperature of 28°C and a speed of 120 r / min After culturing for 3 days, the filtrate was collected after being filtered through a single layer of gauze to obtain a Piromorpha indica fermentation broth.

[0045] 2. Transplanting of Gerbera chrysanthemum tissue culture seedlings

[0046] Gerbera chrysanthemum tissue culture seedlings with a height of 3-4 cm were selected and cultured in the rooting medium for 15 consecutive generations for 30 days. After opening the cover and hardening the seedlings for 2 days, transplant them into flowerpots filled with 3 / 4 humus, one plant per pot. The pots used ar...

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Abstract

The invention provides a method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii; irrigating with Piriformospora indica is performed, and Piriformospora indica is used to promote rooting of the tissue-cultured transplant seedlings of Gerbera jamesonii, improve the roots and promote the growth of plants; in addition, the method may also solve the problems of regenerated plant root weakness, low growth speed, seedling weakness and the like due to multiple subculture, and is significant to promoting the commodity value of Piriformospora indica plants. The method has simple steps, is highly operable and has significant effect.

Description

technical field [0001] The invention relates to the technical field of gerbera chrysanthemum seedling cultivation, in particular to a method for promoting rooting of gerbera chrysanthemum tissue culture transplanted seedlings. Background technique [0002] Gerbera is a perennial herb of Compositae and one of the top five cut flowers in the world. Gerbera does not self-pollinate. Traditional gerbera production often adopts the method of ramet propagation, but the reproductive coefficient of ramet propagation is low, easy to accumulate diseases, the offspring of ramets grow unevenly, the seedlings are weak, and the variety is easy to degenerate. Tissue culture seedlings have the advantages of stable genetic properties, uniform growth, fast growth, and strong stress resistance. Therefore, tissue culture seedlings are mostly used in domestic and foreign gerbera production. If the root system of tissue culture transplanted seedlings is not well developed, it will seriously affec...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程春振郝向阳林觅赖钟雄李丹林玉玲孙雪丽
Owner FUJIAN AGRI & FORESTRY UNIV
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