A method for combined control of tobacco bacterial wilt by utilizing Pyriformis indica and trichloroisocyanuric acid

A technology of trichloroisocyanuric acid and piriformis indica, applied in the field of joint prevention and treatment of tobacco bacterial wilt by using piriformis indica and trichloroisocyanuric acid, can solve the problems that anti-bacteria and pesticides cannot be used together, and achieve improvement The effect of prevention and control, the effect of consolidating the prevention of diseases, and reducing the occurrence of bacterial wilt

Active Publication Date: 2019-01-15
ZHEJIANG UNIV
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Problems solved by technology

[0008] The invention provides a method for jointly preventing and treating tobacco bacterial wilt by utilizing Piriformis indica and trichloroisocyanuric acid, which combines the biological control of Piriformis indica and the chemical control of pesticide trichloroisocyanuric acid, and overcomes the problems in the prior art. The defect that biocontrol bacteria and pesticides cannot be used together

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  • A method for combined control of tobacco bacterial wilt by utilizing Pyriformis indica and trichloroisocyanuric acid
  • A method for combined control of tobacco bacterial wilt by utilizing Pyriformis indica and trichloroisocyanuric acid
  • A method for combined control of tobacco bacterial wilt by utilizing Pyriformis indica and trichloroisocyanuric acid

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Embodiment Construction

[0041] The present invention will be further described below in conjunction with specific examples.

[0042]Materials used in the present invention: Indian piriformis (P.india) was donated by Professor Ajit Verma of the University of Edinburgh in India; Gift from researcher Feng Jie; China Tobacco 100 seeds provided by Zhengzhou Tobacco Research Institute;

[0043] 42% trichloroisocyanuric acid was purchased from Hunan Marine Biological Engineering Co., Ltd.

[0044] 1 Plate Determination of Bacteriostatic Effect on Ralstonia solanacearum

[0045] 1.1 Bacterial suspension preparation

[0046] The strong pathogenic strain RS-TB3 preserved in sterile water was placed on a TTC plate (TTC medium formula: beef extract 3.0g, yeast extract 1g, peptone 5g, glucose 10g, agar 20g, distilled water 1000mL, pH 6.8-7.2. Sterilize at 121°C for 15 minutes, cool to 50°C, add triphenyl tetraazolium chloride salt to make the final concentration reach 50 mg / L) and draw a line on it. After cult...

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Abstract

The invention discloses a method for controlling tobacco bacterial wilt through combination of Piriformospora indica with trichloroisocyanuric acid. The method comprises steps as follows: (1) sowing tobacco seeds in a seedling culture medium mixed with Piriformospora indica mycelia, and seedlings are transplanted to a field; (2) before transplantation, watering holes for tobacco seedling transplantation with a trichloroisocyanuric acid solution, and 10-15 days after transplantation, performing root-irrigation treatment with the trichloroisocyanuric acid solution. The used Piriformospora indica is not inhibited by the pesticide trichloroisocyanuric acid, can be combined with the trichloroisocyanuric acid for use and is applied to control on the tobacco bacterial wilt. The Piriformospora indica and the trichloroisocyanuric acid are combined for use to play a synergistic role in controlling the bacterial wilt, and the control effect is significantly superior to that of the single Piriformospora indica or the single trichloroisocyanuric acid.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to a method for jointly preventing and treating tobacco bacterial wilt by utilizing Pyromorpha indica and trichloroisocyanuric acid. Background technique [0002] Tobacco bacterial wilt is an important soil-borne bacterial disease on tobacco. At present, most of the prevention and control methods focus on chemical control. The use is also easy to cause bacteria to develop drug resistance, which brings difficulties to the continuous prevention and control of diseases. [0003] In recent years, more and more people pay attention to the biological control of plant diseases. Biological control is to use beneficial microorganisms to produce various adverse effects on pathogenic bacteria (antibacterial, bacteriolytic, competition, hyperparasitic) so as to reduce the number of pathogens to weaken their pathogenic bacteria. At the same time, beneficial microorganisms use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00
CPCA01G13/00
Inventor 苏珍珠楼兵干王洪凯曹以衬高其康林福呈
Owner ZHEJIANG UNIV
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