Study method of functions of acid phosphatase in symbiosis of alfalfa root nodules and alfalfa mycorrhiza

Abstract

The invention discloses a study method of functions of acid phosphatase in symbiosis of alfalfa root nodules and alfalfa mycorrhiza, and belongs to the field of studies on legumes. The study method ofthe functions of the acid phosphatase in the symbiosis of the alfalfa root nodules and the alfalfa mycorrhiza comprises the following steps: 1) selecting medicago truncatula seeds, and carrying out surface sterilizing, forced germinating and sprouting; 2) respectively inoculating the sprouted medicago truncatula with sinorhizobium meliloti and arbuscular mycorrhizal fungi, and carrying out separate pot experiments at different phosphorus concentrations; 3) detecting tissue-specific expression of MtPAP2 in the alfalfa root nodules, tissue-specific expression of MtPAP2 in the alfalfa mycorrhiza, influence of the phosphorus on MtPAP2 gene expression, influence of the phosphorus on the alfalfa root nodules and influence of the phosphorus on the alfalfa mycorrhiza; and 4) studying the roles ofthe sinorhizobium meliloti and the arbuscular mycorrhizal fungi in the symbiosis with the alfalfa by utilizing spatiotemporal expression, promoter localization, overexpression and silencing means. Thus, direct experimental evidence is obtained to provide new insights on molecular mechanisms of plant response to low phosphorus stress and coordination of phosphorus homeostasis in the plants by themselves.

Description

technical field [0001] The invention relates to the technical field of leguminous plant research, in particular to a method for studying the function of acid phosphatase in the symbiosis of alfalfa root nodules and mycorrhizae. Background technique [0002] The symbiotic system formed by legumes and rhizobia or AM fungi is of great significance for the sustainable development of agriculture. The biological nitrogen fixation process of rhizobia and leguminous plants mainly provides the nitrogen source required for plant growth, while AM ​​fungi can improve the phosphorus homeostasis in plants; nitrogen and phosphorus are two macroelements necessary for plant growth and development, and The deficient phosphorus content in soil not only affects the growth of legume crops, but also adversely affects the symbiotic nitrogen fixation of legumes. [0003] At present, the research on the PAP family genes of legumes mostly focuses on assisting plants to cope with phosphorus stress an...

AI Technical Summary

Problems solved by technology

The biological nitrogen fixation process of rhizobia and leguminous plants mainly provides the nitrogen source required for plant growth, while AM ​​fungi can improve the phosphorus homeostasis in plants; nitrogen and phosphorus are two macroelements necessary for plant growth and development, and Deficient phosphorus content in soil not only affects the growth of legumes, but also adversely affects the symbiotic nitrogen fixation of legumes

[0003] At present, the research on the PAP family genes of legumes mostly focuses on assisting plants to cope with phosphorus stress and improving the phosphorus nutrition of plants, but there are few reports on the functions of this family of proteins in the process of plant-microbial symbiosis

Images