Alumite-stress transgenic soybean and application thereof

A technology of transgenic soybean and acid-resistant aluminum, applied in the field of genetic engineering, can solve problems such as quality and yield reduction, aluminum poisoning stress, and delayed crop growth and development.

Inactive Publication Date: 2019-10-22
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, most of the soil in southern my country is acidic red loam, which is prone to al

Method used

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  • Alumite-stress transgenic soybean and application thereof
  • Alumite-stress transgenic soybean and application thereof
  • Alumite-stress transgenic soybean and application thereof

Examples

Experimental program
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Embodiment Construction

[0024] In this example, by heterologously overexpressing the zinc finger transcription factor (C2H2) gene of the model leguminous crop Medicago truncatula, MtSTOP transgenic soybean plants that can significantly improve the tolerance to aluminum acid were obtained.

[0025] 1. Materials

[0026] 1. Medicago trunca tula: R108; soybean (G1ycine max): Tianlong No.1 (PI1578450, 12W12);

[0027] 2. Escherichia coli: DH5α; Agrobacterium rhizogenes: K599;

[0028] 3. Vectors: pGEM-T Easy, pDONR221, pB2GW7;

[0029] 4. LB medium: Weigh 10g of NaCl, 5g of yeast extract, 10g of tryptone, add 950mL of ultrapure water and stir to dissolve, add water to 1000mL, and sterilize by high pressure steam for 15min to obtain LB liquid medium , LB solid medium is to add 15g of agar powder to LB liquid medium.

[0030] 5. Ampicillin mother solution (Amp+, 50mg / ml): Weigh 0.5g ampicillin Amp, dissolve in 10mL sterilized water, filter and sterilize, divide into small tubes, and store at -20°C; Kana...

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Abstract

The invention discloses an alumite-stress transgenic soybean and application thereof. The transgenic soybean is infected by agrobacterium, and exogenous zinc finger protein transcription factors are integrated in a transgenic soybean genome; the amino acid sequence encoded by the zinc finger protein transcription factors is shown in SEQIDNO.1. The transgenic soybean is obtained after the zinc finger protein transcription factors separated from medicago truncatula are transformed and expressed in soybean plants. The production and application of the alumite-stress transgenic soybean are provided and of great significance for cultivating acid-aluminum resistant plant varieties and improving the crop yield in southern acid aluminum soil, and lay a foundation for further research on acid-aluminum resistance of crops.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to an aluminum stress-resistant transgenic soybean and an application thereof. Background technique [0002] Soil pH is very important to crop growth, and most crops prefer neutral or slightly acidic soil. However, in acidic soil, the root growth and development of crops are first damaged, and then the absorption of water and mineral nutrients is affected, which eventually leads to the reduction of crop quality and yield, and the decline of economic benefits. [0003] Acidic soil and aluminum toxicity seriously affect about 30% of the arable land area worldwide, which is one of the main factors restricting food production. There are about 20 million hectares of typical acidic soils such as brick red soil, red soil and red red soil widely distributed in southern my country, which seriously restricts the production of crops. [0004] Soybean is a traditional crop in my c...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/54
CPCC12N15/8271
Inventor 赵剑曾强圣王俊杰
Owner HUAZHONG AGRI UNIV
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