Transcription factor gene for regulating and controlling synthesis of plant flavonoid and use thereof

Abstract

The invention discloses a transcription factor gene for regulating and controlling synthesis of plant flavonoid. The transcription factor gene is an MtbZIP10 gene authenticated from a leguminous plant, namely medicago truncatula, and the gene sequence of the transcription factor gene is represented by SEQ ID NO.1. Furthermore, the invention discloses functions and application of the transcriptionfactor gene. The transcription factor gene has the advantages that the transcription factor gene capable of positively regulating and controlling the synthesis of flavonoid compounds is cloned from the leguminous plant, namely medicago truncatula, the system identification of the functions of the transcription factor gene is carried out, and the system identification result shows that the gene iscapable of activating multiple genes in the biosynthetic pathway of flavonoid and the content of the flavonoid compounds is increased.

Description

technical field [0001] The invention belongs to the technical field of gene cloning, in particular to a transcription factor gene for regulating the synthesis of plant flavonoids and its application. Background technique [0002] Flavonoids are a large class of important secondary metabolites widely distributed in roots, stems, leaves, flowers, fruits and seed coats of plants, including flavonoids, isoflavones, flavonols, anthocyanins and proanthocyanidins, etc. . Among them, anthocyanins have the functions of protecting plants from biological invasion and attracting insect pollination. Anthocyanins can also respond to biotic and abiotic stress, scavenge oxygen free radicals, and protect plants from high-density light damage. At the same time, anthocyanins have certain health care functions in anti-cancer, disease resistance and anti-oxidation. Proanthocyanidins, another type of flavonoids, not only protect plants from herbivores and pathogenic bacteria, but also can effec...

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Problems solved by technology

[0004] Aiming at the problem of insufficient bZIP transcription factor genes in the biosynthesis pathway of flavonoids in the existing research, and it is difficult to increase the content of flavonoids in a large amount, the present invention provides a transcription factor gene for regulating the synthesis of flavonoids in plants and its application. The gene is a New gene, the bZIP family transcription factors that regulate the synthesis of flavonoids (anthocyanidins and proanthocyanidins) have not been found in Medicago truncatula. Therefore, it is necessary to study the bZIP transcription factors that regulate the biosynthesis of flavonoids in Medicago truncatula

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