Transcription factor gene for regulating and controlling synthesis of plant flavonoid and use thereof

A technology for transcription factors and flavonoids, which is applied in the fields of plant genetic improvement, plant products, genetic engineering, etc., can solve the problems of insufficient bZIP transcription factor genes, and it is difficult to increase the content of flavonoids in a large amount, and achieve the effect of increasing the content.

Active Publication Date: 2019-03-19
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

[0004] Aiming at the problem of insufficient bZIP transcription factor genes in the biosynthesis pathway of flavonoids in the existing research, and it is difficult to increase the content of flavonoids in a large amount, the present invention provides a transcription factor gene for regulating the synthesis of flavonoids in plants a...

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  • Transcription factor gene for regulating and controlling synthesis of plant flavonoid and use thereof
  • Transcription factor gene for regulating and controlling synthesis of plant flavonoid and use thereof
  • Transcription factor gene for regulating and controlling synthesis of plant flavonoid and use thereof

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0019] 1. Main experimental materials and methods

[0020] 1.1 Genomic DNA extraction from Medicago truncatula by CTAB method

[0021] 1) Take about 100 mg of Medicago truncatula tissue material and place it together with steel balls in a 2 mL centrifuge tube; add liquid nitrogen and shake continuously until the tissue material is fully ground into powder.

[0022] 2) Add 500 μL CTAB extract (preheated at 65°C), and vortex to mix well.

[0023] 3) The mixture was placed in a...

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Abstract

The invention discloses a transcription factor gene for regulating and controlling synthesis of plant flavonoid. The transcription factor gene is an MtbZIP10 gene authenticated from a leguminous plant, namely medicago truncatula, and the gene sequence of the transcription factor gene is represented by SEQ ID NO.1. Furthermore, the invention discloses functions and application of the transcriptionfactor gene. The transcription factor gene has the advantages that the transcription factor gene capable of positively regulating and controlling the synthesis of flavonoid compounds is cloned from the leguminous plant, namely medicago truncatula, the system identification of the functions of the transcription factor gene is carried out, and the system identification result shows that the gene iscapable of activating multiple genes in the biosynthetic pathway of flavonoid and the content of the flavonoid compounds is increased.

Description

technical field [0001] The invention belongs to the technical field of gene cloning, in particular to a transcription factor gene for regulating the synthesis of plant flavonoids and its application. Background technique [0002] Flavonoids are a large class of important secondary metabolites widely distributed in roots, stems, leaves, flowers, fruits and seed coats of plants, including flavonoids, isoflavones, flavonols, anthocyanins and proanthocyanidins, etc. . Among them, anthocyanins have the functions of protecting plants from biological invasion and attracting insect pollination. Anthocyanins can also respond to biotic and abiotic stress, scavenge oxygen free radicals, and protect plants from high-density light damage. At the same time, anthocyanins have certain health care functions in anti-cancer, disease resistance and anti-oxidation. Proanthocyanidins, another type of flavonoids, not only protect plants from herbivores and pathogenic bacteria, but also can effec...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00A01H6/54
CPCC12N9/1085C12N15/825
Inventor 庞永珍宋雪薇狄少康
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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