16 results about "Flavonoid synthesis" patented technology

This invention belongs to the fields of genetic engineering and plant cultivation technology, specifically relating to a method for inducing flavonoid synthesis in kale through selenium induction. BroMYB34 Genes and their applications. Specifically, this invention is the first to identify and clone a selenium-induced gene in kale. BroMYB34 The gene was used to elucidate its molecular function as a transcription factor regulating flavonoid synthesis through overexpression. BroMYB34 Genes can significantly increase the flavonoid content of kale and enhance its antioxidant capacity, providing an efficient and targeted genetic engineering approach for quality improvement of kale; at the same time, BroMYB34 Genes in cruciferous plants have potential functional conservation and can be extended to the quality improvement of various vegetables. This is of great significance for promoting the breeding and industrialization of functional vegetables and therefore has good practical application value.

The invention discloses a culture method for increasing the flavonoid content of suspension cells of codonopsis pilosula. The method comprises the following steps: (1) obtaining aseptic seedlings: disinfecting codonopsis pilosula seeds, inoculating the disinfected codonopsis pilosula seeds into an MS culture medium, and culturing to obtain the aseptic seedlings; (2) callus induction and subculture: taking the roots of the aseptic seedlings as explants, and performing induction and subculture on an MS culture medium containing 2, 4-D, 6-BA and KT to obtain loose calluses; (3) suspension culture: inoculating the callus into an MS liquid culture medium containing NAA, 6-BA and KT for suspension culture to obtain high-biomass codonopsis pilosula suspension cells; and (4) adding an elicitor / precursor: adding methyl jasmonate (MeJA) or sodium acetate (NaAC) into the suspension culture system, and inducing flavone synthesis and accumulation. By optimizing a plant growth regulator composition in the culture medium, the biomass of the suspension cells of the codonopsis pilosula is remarkably improved (the highest biomass reaches 57.57 g / L); by adding MeJA or NaAC, the daily average yield of flavone is increased to 16.69 mg / L / d, and the daily average yield of flavone is increased to 18.20 mg / L / d. According to the method, the culture period is about 70 days, and a feasible way is provided for efficient and large-scale production of codonopsis pilosula flavone.

The application belongs to the technical field of biology, and discloses a transcription factor SlNAC078 for regulating flavonoid synthesis of tomatoes and application. The CDS sequence of the transcription factor SlNAC078 is shown as SEQ ID NO. 1, the transcription factor SlNAC078 can positively regulate flavonoid synthesis of tomatoes, overexpression of the gene can activate the promoters of SlCHS1, SlCHS2 and SlFLS genes, and significantly improve the flavonoid content in fruits; and the transcription factor SlNAC078 can also improve the root dry weight and single fruit weight of tomato plants, and realize the synergistic improvement of nutritional quality and yield. The transcription factor SlNAC078 for regulating flavonoid synthesis of tomatoes and application directly targets the regulation mechanism of key genes for flavonoid synthesis, the cultivation method is simple, the obtained transgenic line is genetically stable, and the transcription factor SlNAC078 provides a key gene target and technical scheme for tomato breeding, and has an important application prospect in the industrialized production of high-nutrition and high-yield tomato products.

The invention discloses a gingko GbF3H gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbF3H gene is obtained by analyzing and screening on the basis of ginkgo transcriptome data in the earlier stage of a research group, the GbF3H gene is flavanone 3-hydroxylase, the nucleotide sequence of the GbF3H gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbF3H gene is shown as SEQ ID NO.2. A GbF3H overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the difference of 28 flavonoid metabolites is obvious, and the contents of 9 flavonoid substances such as formononin are up-regulated. The invention provides a theoretical basis, a valuable gene resource and a method for analyzing a ginkgo flavone synthesis mechanism and improving the flavone yield through metabolic engineering, and has important significance on development and utilization of ginkgo resources and cultivation of new germplasm with medicinal value.

The invention discloses application of Fe < 2 + >-loaded mesoporous nano silicon dioxide to increase the yield of rice, and belongs to the technical field of plant growth regulators. The Fe < 2 + >-loaded mesoporous nano silicon dioxide improves the oxidation resistance of the rice by activating a flavonoid synthesis route in the colored rice, so that excessive active oxygen is effectively removed, protein oxidative damage is relieved, cell redox balance is maintained, the thousand grain weight loss and the maturing rate of the rice under high temperature stress are improved, and the rice quality is improved. The yield of the rice under the high-temperature heat damage resistance is improved.

The invention discloses an MYB transcription factor LrMYB12 related to synthesis of a lycium ruthenicum flavonoid substance and application of the MYB transcription factor LrMYB12. Belongs to the technical field of gene engineering. According to the invention, the LrMYB12 transcription factor closely related to flavonoid synthesis and the coding gene thereof are cloned and identified from lycium ruthenicum for the first time, a new key component is supplemented for analyzing a transcription regulation network for lycium ruthenicum flavonoid biosynthesis, and the blank in the prior art is filled. The LrMYB12 gene disclosed by the invention can be used as an important functional gene, is used for creating a new lycium barbarum germplasm with significantly increased flavonoid content through modern molecular breeding technologies such as transgenosis, gene editing or molecular marker-assisted selection, and has clear application prospects and industrial values in the aspects of functional food development and medicinal material quality improvement.

The present application relates to the technical field of genetic engineering, and particularly relates to application of VvbHLH68 gene and VvERF104 gene in regulation of anthocyanin synthesis in grape. The VvbHLH68 gene transcription factor is subcellularly located in the nucleus, and specific interaction exists between the VvbHLH68 gene and the VvERF104 gene, which is verified by yeast two-hybrid and luciferase complementation assay. The co-transformation test shows that the VvbHLH68 gene and the VvERF104 gene can significantly enhance the anthocyanin and flavonoid synthesis capacity of grape callus in synergistic action.

This invention relates to the field of biotechnology, specifically to the construction and application of a Benzodiacetic tobacco chassis for synthesizing isoflavones. This invention reconstructs the upstream pathway for isoflavone synthesis in Benzodiacetic tobacco, further identifies and screens key rate-limiting enzymes for isoflavone synthesis in Benzodiacetic tobacco, and utilizes 2A peptides to co-express multiple key genes, further introducing flavonoid synthesis transcription factors to construct a Benzodiacetic tobacco chassis for synthesizing isoflavones. This Benzodiacetic tobacco chassis can efficiently synthesize daidzein and genistein. Furthermore, based on the constructed high-yield Benzodiacetic tobacco platform for daidzein and genistein, various isoflavones can be further synthesized, such as daidzein, genistein, puerarin, gentiopicrin, chickpea extract A, and stigmosiderin, paving the way for the industrialization of plant biosynthesis of various bioactive isoflavones and thus possessing broad application prospects.

The application discloses a kind of based on hairy root agrobacterium and RUBY reporter gene-mediated visible genetic transformation method of Radix Ternatae, for the technical obstacle that Radix Ternatae lignification degree is high, agrobacterium is difficult to infect, the application uses the stem base of Radix Ternatae as explant, using the hairy root agrobacterium K599 carrying RUBY reporter gene is infected, through vacuum filtration combination darkness soaking, directly transplanting to nutrient soil after being infected explant, without relying on tissue culture and sterile environment.The application realizes the rapid induction and visual screening of Radix Ternatae transgenic hairy root, and the average 21 days appears and presents characteristic red, and positive induction rate can reach 20%.The application also discloses the function of new gene ThFLS116 of Radix Ternatae for the first time, which regulates root development and flavonoid synthesis metabolism.The application provides an efficient technical platform for gene function analysis and active ingredient industrialization development of Radix Ternatae.

The invention discloses a method for improving the flavone content and oxidation resistance of auricularia polytricha by using 6-benayl aminopurine, and belongs to the technical field of edible fungus cultivation. It is found and confirmed for the first time that 10 mg / L of 6-BA can significantly improve the flavone content and in-vitro oxidation resistance (DPPH and FRAP) of auricularia polytricha mycelia, and does not have an inhibition effect on mycelia growth. Through conjoint analysis of transcriptome and metabonomics, the molecular mechanism that 6-BA promotes accumulation of precursor substances by up-regulating expression of key genes such as PAL, 4CL, DFR and OMT in a flavonoid biosynthetic pathway so as to drive flavonoid synthesis is clarified from the molecular level. The invention provides an effective method for improving the nutritional value of the auricularia polytricha and developing functional products, and lays a theoretical foundation for the subsequent directional regulation and control of the flavone synthesis of the auricularia polytricha by means of genetic engineering, metabolic engineering and the like.

The invention belongs to the technical field of gene engineering and plant cultivation, and particularly relates to a BroMYB34 gene synthesized by inducing kale flavonoid through selenium and application of the BroMYB34 gene. Specifically, the selenium-induced BroMYB34 gene in the kale is identified and cloned for the first time, the molecular function of the selenium-induced BroMYB34 gene serving as a transcription factor for regulating and controlling flavonoid synthesis is clarified, and the BroMYB34 gene is over-expressed, so that the flavonoid content of the kale can be remarkably increased, the oxidation resistance of the kale can be enhanced, and the application prospect is broad. An efficient and oriented genetic engineering technical approach is provided for the quality improvement of the kale; meanwhile, the BroMYB34 gene has potential function conservative property in cruciferous plants, can be expanded and applied to quality improvement of various vegetables, and has important significance in promoting breeding and industrialization of functional vegetables, so that the BroMYB34 gene has good practical application value.

The invention discloses a phyllostachys pubescens flavone synthase I gene PeFNSI1, an encoded protein and an application of the phyllostachys pubescens flavone synthase I gene PeFNSI1, belongs to the technical field of genetic engineering, and particularly relates to the phyllostachys pubescens flavone synthase I gene PeFNSI1, the nucleotide sequence of the phyllostachys pubescens flavone synthase I gene PeFNSI1 is shown as SEQ ID NO.1, and the application of the phyllostachys pubescens flavone synthase I gene PeFNSI1 in enhancing The invention reveals for the first time that flavone synthetase I (PeFNSI) coded by the phyllostachys pubescens PeFNSI1 gene can specifically catalyze flavone precursor naringenin to be converted into apigenin and catalyze eriodictyol to be converted into luteolin, and can activate a'bifunctional 'regulation element of a flavone synthesis and salicylic acid defense pathway at the same time by reprogramming salicylic acid metabolic flux, so that the flavone synthetase I (PeFNSI) can be used for preparing the apigenin and the luteolin. The multi-element synergistic effect of flavone synthesis, salicylic acid metabolic flux regulation and control and disease resistance enhancement is achieved, the limitation of an existing disease resistance strategy is broken through, and a brand new molecular tool is provided for plant disease resistance breeding.

This invention belongs to the field of bioengineering technology and provides a method for screening transcription factors that regulate flavonoid synthesis in kiwifruit and its application. This invention systematically evaluates the 'Hongyang' kiwifruit variety using transcriptome sequencing and dual-luciferase analysis, assessing the activation effects of ERF122 and HZP66 proteins on key genes involved in flavonoid synthesis. Results show that both ETH and 1-MCP significantly promote flavonoid accumulation. Transcriptome sequencing identified 285 differentially expressed genes and identified 10 potential regulatory transcription factors, among which ERF122 and HZP66 proteins can directly bind to and activate the promoters of the key flavonoid synthesis genes AcPAL1 / 2, respectively. This invention improves the molecular regulatory network of flavonoid metabolism in kiwifruit and provides theoretical support and molecular targets for related breeding and postharvest quality control.

The invention discloses a transcription factor BnaMYB30 and application thereof, and belongs to the technical field of gene engineering. The invention provides application of a BnaMYB30 gene or protein to regulation and control of drought resistance of rape and / or regulation and control of anthocyanin content of rape under drought stress. The BnaMYB30 is combined with a BnaUGT75C1 promoter and a BnaFLS1 promoter to participate in regulation and control of glycosylation of anthocyanin and flavonol synthesis, and accumulation of the anthocyanin is remarkably promoted by activating key structural genes CHI, F3H and DFR in a flavonoid synthesis pathway. The BnaMYB30 overexpression strain lacks effective ABA and JA hormone signal regulation and control, and the stress response mode is single, so that the antioxidant enzyme activity is reduced, the active oxygen scavenging capacity is reduced, and finally the drought sensitive phenotype is shown.

This invention discloses a miR3980b Its application in promoting flavonoid synthesis in rice grains belongs to the field of genetic engineering technology. This invention is the first to discover and verify this technology. miR3980b It is a negative regulator of flavonoid biosynthesis in rice. Through positive (overexpression) and negative (STTM silencing) genetic strategies, combined with physiological and biochemical assays and metabolomics analysis, it was confirmed that... miR3980b Negative regulatory function in flavonoid accumulation in rice grains. Through silencing... miR3980b , miR3980b The expression level was significantly reduced, while the total flavonoid and polyphenol content was significantly increased, further confirming... miR3980b miR3980b Negative regulation of flavonoid biosynthesis. This invention lays the theoretical foundation for cultivating rice varieties with increased flavonoid content.

The invention belongs to the technical field of gene engineering, and particularly relates to application of Gb-miR858a-GbMYBs in regulation and control of synthesis of ginkgo flavonoids. According to the invention, one miR858 and three MYBs target genes are screened from gingko miRNA and transcriptome sequencing data, and functions of candidate genes in gingko flavonoid metabolic pathways are studied through technologies of bioinformatics analysis, gene cloning, genetic transformation and the like. The invention discloses an interaction relationship between Gb-miR858a and GbMYBs target genes of ginkgo biloba, researches prove that GbMYB1, GbMYB39 and GbMYB41 are all forward regulatory factors for flavonoid synthesis, a regulation mechanism of Gb-miR858a targeting GbMYBs participating in flavonoid synthesis is preliminarily clarified, and a theoretical basis is provided for increasing the content of ginkgo flavonoid by adopting a genetic engineering technology.