31 results about "Flavonoid synthesis" patented technology

There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

The invention relates to a tulip flavanone-3-hydroxylase TfF3H protein and an encoding gene thereof. The protein is composed of amino acid sequences represented by the SEQ ID No.4, or the protein is composed of amino acid sequences which have been processed by replacing, removing, or adding one or several amino acids, and has a tulip flavanone-3-hydroxylase activity. The invention also provides a nucleic acid sequence which encodes the protein mentioned above. The flavanone-3-hydroxylase is an important branch point catalyzing enzyme in the flavonoid synthesis route, and is a pivot of a whole flavonoid compound metabolic route. After the tulip flavanone-3-hydroxylase gene is transferred to orange red morning glories through establishing an antisense expression vector, flower color of more than 50% of genetically modified morning glories becomes lighter, and the total anthocyanin content of corresponding flowers is reduced by 30% to 50%. By utilizing the tulip-flavanone-3-hydroxylase gene TfF3H, people can change the flower color by utilizing a gene engineering technology, and obtain an effective technical means for creating novel flower colors.

The invention discloses a peony PsMYB12 transcription factor as well as an encoding gene and application thereof. The peony PsMYB12 transcription factor is a protein of (a) or (b) which is described as follows: (a) a protein consists of an amino acid sequence shown by a sequence 1 in a sequence table; (b) proteins which are derived from (a), are associated with peony color spot formation and / or anthocyanin synthesis and are formed by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence shown by the sequence 1 in the sequence table. By means of VIGSsilencing and heterologous excess transformation analysis, the results show that the peony PsMYB12 transcription factor can regulate the expression of key enzyme genes in an anthocyanin synthesis route. The peony PsMYB12 transcription factor is speculated to play an important role in the synthesis and accumulation of anthocyanin in peony flower spots and the formation of color spots. The specifically expressed gene is cloned for the first time and the functions of the gene are verified, so that a foundation is laid for the molecular mechanism of the formation of the color spots, and technicalbasis and thought are provided for further analysis of the synthesis and regulation of flavonoids in peony flowers.

The invention belongs to the technical field of genetic engineering application and particularly relates to application of an Arabidopsis thaliana TT4 gene (Arabidopsis thaliana genome code At5g13930) to culture of new varieties of salt-resistant plants. The TT4 gene with a CDS length being 1188bp encodes proteins of 395 amino acids and is related to seed activity, and the gene enables release of Arabidopsis thaliana seed activity after genetic mutation, namely the gene is capable of enhancing seed activity to accelerate seed germination and improve seed germinability after genetic mutation. The TT4 gene mainly serves as a first specific enzyme in a flavonoid synthesis route and participates in regulation of development of seed coats and seed coat pigments of Arabidopsis thaliana. According to researches of the TT4 gene in simulated dry or high-salinity adverse situations, plants under specific regulation of the gene has an osmotic stress reaction to salts and the like, and after silencing of the gene, mutant plants show favorable salt resistance at an initial germination stage.