31 results about "Flavonoid synthesis" patented technology

There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

The invention relates to a tulip flavanone-3-hydroxylase TfF3H protein and an encoding gene thereof. The protein is composed of amino acid sequences represented by the SEQ ID No.4, or the protein is composed of amino acid sequences which have been processed by replacing, removing, or adding one or several amino acids, and has a tulip flavanone-3-hydroxylase activity. The invention also provides a nucleic acid sequence which encodes the protein mentioned above. The flavanone-3-hydroxylase is an important branch point catalyzing enzyme in the flavonoid synthesis route, and is a pivot of a whole flavonoid compound metabolic route. After the tulip flavanone-3-hydroxylase gene is transferred to orange red morning glories through establishing an antisense expression vector, flower color of more than 50% of genetically modified morning glories becomes lighter, and the total anthocyanin content of corresponding flowers is reduced by 30% to 50%. By utilizing the tulip-flavanone-3-hydroxylase gene TfF3H, people can change the flower color by utilizing a gene engineering technology, and obtain an effective technical means for creating novel flower colors.

The invention discloses a peony PsMYB12 transcription factor as well as an encoding gene and application thereof. The peony PsMYB12 transcription factor is a protein of (a) or (b) which is described as follows: (a) a protein consists of an amino acid sequence shown by a sequence 1 in a sequence table; (b) proteins which are derived from (a), are associated with peony color spot formation and / or anthocyanin synthesis and are formed by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence shown by the sequence 1 in the sequence table. By means of VIGSsilencing and heterologous excess transformation analysis, the results show that the peony PsMYB12 transcription factor can regulate the expression of key enzyme genes in an anthocyanin synthesis route. The peony PsMYB12 transcription factor is speculated to play an important role in the synthesis and accumulation of anthocyanin in peony flower spots and the formation of color spots. The specifically expressed gene is cloned for the first time and the functions of the gene are verified, so that a foundation is laid for the molecular mechanism of the formation of the color spots, and technicalbasis and thought are provided for further analysis of the synthesis and regulation of flavonoids in peony flowers.

The invention relates to a gene for regulating the synthesis of flavonoids, its coded protein and its application, and belongs to the technical field of plant genetic engineering. The gene regulating flavonoid synthesis, the sequence is shown in SEQ ID NO: 1, which is derived from common tobacco, named NtMYB27, the NtMYB27 gene is 1240bp in length, contains 3 introns and 4 exons, and its coding region is complete 597 bp long. The present invention finds that the NtMYB27 transcription factor can inhibit the synthesis of flavonols in leaves through cloning analysis of gene sequences, construction of expression vectors, and gene expression detection, and this inhibition is achieved by inhibiting the expression of NtFLS and NtCHS genes of. Therefore, the present invention confirms that NtMYB27 is a negative regulator of plant flavonol synthesis. When the expression of this gene is inhibited or its function is lost, it can significantly increase the content of flavonols in plant leaves, providing a basis for the subsequent use of gene editing technology to enhance plant flavonols. Assays provide clear target genes.

The invention discloses a key gene GbMYB6 for promoting the synthesis of ginkgo flavonoids and its expressed protein, carrier and application. The nucleotide sequence of the key gene GbMYB6 is shown in SEQ ID NO.1, and the amino acid of the expressed protein is The sequence is shown in SEQ ID NO.2. The present invention uses Ginkgo biloba leaves as materials, clones the GbMYB6 gene, constructs an overexpression vector 35S::GbMYB6 containing the gene GbMYB6 by enzyme cleavage, and transfers it into Ginkgo callus and Arabidopsis thaliana, GbMYB6 transgenic Ginkgo callus and transgene The content of flavonoids in Arabidopsis thaliana was significantly increased, which indicated that GbMYB6 can promote the synthesis of flavonoids. Therefore, regulating the expression of GbMYB6 has important application value in improving the medicinal quality of Ginkgo biloba leaves.

The invention discloses a transcription factor gene that regulates the synthesis of plant flavonoids. The transcription factor gene is the MtbZIP10 gene identified from the leguminous plant Medicago truncatula, and its gene sequence is shown in SEQ ID NO.1. Moreover, the present invention discloses the function of the gene and its application. The advantage of the present invention is that the transcription factor gene that can positively regulate the synthesis of flavonoid compounds is cloned from the leguminous plant Medicago truncatula, and its function is systematically identified, and it is found that the gene can activate the biosynthesis pathway of flavonoids Multiple genes that increase the content of flavonoid compounds.

The invention provides a chalcone isomerase gene of asparaguses, a protein encoded by the chalcone isomerase gene and application of the chalcone isomerase gene. The CDS sequence of the chalcone isomerase gene AoCHI1 is shown as SEQ ID NO: 1; the amino acid sequence of the protein encoded by the gene is represented by SEQ ID NO: 2. The chalcone isomerase gene AoCHI1 is cloned from the asparagus for the first time, and is one of key genes for synthesizing plant flavonoids; by the adoption of a method of gene engineering, the gene AoCHI1 is transferred into a target plant, so that the total flavonoids content of a transgenic plant can be increased, to improve the plant quality by using a gene engineering technology in the future; furthermore, an important theoretical basis is provided for obtaining medicines or food with high oxidization resistance, and the chalcone isomerase gene has a wide application prospect and high economic value.

The invention belongs to the technical field of genetic engineering application and particularly relates to application of an Arabidopsis thaliana TT4 gene (Arabidopsis thaliana genome code At5g13930) to culture of new varieties of salt-resistant plants. The TT4 gene with a CDS length being 1188bp encodes proteins of 395 amino acids and is related to seed activity, and the gene enables release of Arabidopsis thaliana seed activity after genetic mutation, namely the gene is capable of enhancing seed activity to accelerate seed germination and improve seed germinability after genetic mutation. The TT4 gene mainly serves as a first specific enzyme in a flavonoid synthesis route and participates in regulation of development of seed coats and seed coat pigments of Arabidopsis thaliana. According to researches of the TT4 gene in simulated dry or high-salinity adverse situations, plants under specific regulation of the gene has an osmotic stress reaction to salts and the like, and after silencing of the gene, mutant plants show favorable salt resistance at an initial germination stage.

The invention discloses a key gene GbMYB4 that regulates the synthesis of ginkgo flavonoids and its expressed protein, carrier and application. The nucleotide sequence of the key gene GbMYB4 is shown in SEQ ID NO.1, and the amino acid of the expressed protein is The sequence is shown in SEQ ID NO.2. The present invention uses Ginkgo leaves as materials, clones the GbMYB4 gene, constructs an overexpression vector containing the gene GbMYB4 and transfers it into Ginkgo callus and Arabidopsis thaliana, and the flavonoids in the GbMYB4 transgenic Ginkgo callus and transgenic Arabidopsis of the present invention The contents of the flavonoids were significantly reduced, which indicates that GbMYB4 can inhibit the synthesis of flavonoids, negatively regulate flavonoids, and cooperate with genes that promote flavonoid synthesis to effectively control the synthesis of flavonoids such as Ginkgo biloba, thus regulating the expression of GbMYB4 It has important application value in improving the medicinal quality of Ginkgo biloba leaves.