31 results about "Flavonoid synthesis" patented technology

The invention discloses engineering lactobacilli for producing plant flavonoid to synthesize related enzymes, construction and application thereof, belonging to the field of lactobacillus reformation of genetic engineering. The engineering lactobacilli is produced by the steps of: synthesizing flavonoid completely from Chinese locally planted soybeans (Glycine max(L.)Merr.) into related enzyme genes PAL, 4CL, CHS and CHI which are connected into a polycistron structure PLA-4CL-CHS-CHI; then constructing the polycistron structure onto a reformed lactobacillus expression vector pMG26e to form a recombinant vector pMG26e-4GS; and then electrically shocking and converting the pMG26e-4GS into lactobacilli to obtain the engineering lactobacilli for producing plant flavonoid to synthesize related enzymes. The preservation number of the recombinant lactobacilli is CGMCC (China General Microbiological Culture Collection Center) No.4086, and the engineering lactobacilli has more abundant physiological functions compared with original lactobacilli and can be applied to the field of food. Through fermentation, the flavonoid output of the obtained recombinant strain is in the level of mg / l, which is higher than the fermentation levels of like recombinant escherichia coli and recombinant yeast.

There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

The invention discloses a key gene GbMYB6 for promoting synthesis of gingko flavonoids, protein expressed by the key gene GbMYB6, a carrier and application of the key gene GbMYB6, the nucleotide sequence of the key gene GbMYB6 is shown as SEQ ID NO.1, and the amino acid sequence of the protein expressed by the key gene GbMYB6 is shown as SEQ ID NO.2. Ginkgo biloba leaves are used as materials, a GbMYB6 gene is cloned, an overexpression vector 35S:: GbMYB6 containing the gene GbMYB6 is constructed through enzyme digestion connection and transferred into ginkgo biloba calluses and arabidopsis thaliana, and the content of flavonoids in GbMYB6 transgenic ginkgo biloba calluses and transgenic arabidopsis thaliana is obviously increased, this indicates that GbMYB6 can promote the synthesis of flavonoids, the regulation and control of the expression of the GbMYB6 has important application value in the aspects of improving the medicinal quality of the ginkgo leaf tablets and the like.

The invention relates to a tulip flavanone-3-hydroxylase TfF3H protein and an encoding gene thereof. The protein is composed of amino acid sequences represented by the SEQ ID No.4, or the protein is composed of amino acid sequences which have been processed by replacing, removing, or adding one or several amino acids, and has a tulip flavanone-3-hydroxylase activity. The invention also provides a nucleic acid sequence which encodes the protein mentioned above. The flavanone-3-hydroxylase is an important branch point catalyzing enzyme in the flavonoid synthesis route, and is a pivot of a whole flavonoid compound metabolic route. After the tulip flavanone-3-hydroxylase gene is transferred to orange red morning glories through establishing an antisense expression vector, flower color of more than 50% of genetically modified morning glories becomes lighter, and the total anthocyanin content of corresponding flowers is reduced by 30% to 50%. By utilizing the tulip-flavanone-3-hydroxylase gene TfF3H, people can change the flower color by utilizing a gene engineering technology, and obtain an effective technical means for creating novel flower colors.

The invention discloses a plant flavonoid synthesis regulation gene and its application. CDNA obtained through the inverse transcription of the total RNA of Epimedium brevicornum Maxim blades is treated as a template and undergoes PCR to obtain a 233bp fragment, the 233bp fragment has a very high homology with flavonoid related R2R3-MYB gene, a full-length cDNA sequence is obtained through an RACE technology and is named EsMYBA1 gene, and the sequence of the EsMYBA1 gene is represented by SEQIDNO.1. The EsMYBA1 gene codes an R2R3-MYB transcription factor which has an about 50% homology with other MYB regulation genes for controlling the flavonoid synthesis approaches, and the improvement of the expression level of the EsMYBA1 gene through a genetic engineering technology promotes the synthesis and the accumulation of anthocyanidin.

The invention discloses a peony PsMYB12 transcription factor as well as an encoding gene and application thereof. The peony PsMYB12 transcription factor is a protein of (a) or (b) which is described as follows: (a) a protein consists of an amino acid sequence shown by a sequence 1 in a sequence table; (b) proteins which are derived from (a), are associated with peony color spot formation and / or anthocyanin synthesis and are formed by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence shown by the sequence 1 in the sequence table. By means of VIGSsilencing and heterologous excess transformation analysis, the results show that the peony PsMYB12 transcription factor can regulate the expression of key enzyme genes in an anthocyanin synthesis route. The peony PsMYB12 transcription factor is speculated to play an important role in the synthesis and accumulation of anthocyanin in peony flower spots and the formation of color spots. The specifically expressed gene is cloned for the first time and the functions of the gene are verified, so that a foundation is laid for the molecular mechanism of the formation of the color spots, and technicalbasis and thought are provided for further analysis of the synthesis and regulation of flavonoids in peony flowers.

The invention discloses a wheat salt-resistant gene, i.e., a wheat secondary metabolism flavone synthetic route gene TaFLS and a plant expression vector pSTART-TaFLS containing the gene TaFLS. The invention further discloses application of an expression vector of the gene to the cultivation of salt-resistant and oxidation-resistant plants. As proved by an experiment, the salt resistance and the oxidation resistance of a transgenic plant are remarkably enhanced.

The invention relates to a gene for regulating the synthesis of flavonoids, its coded protein and its application, and belongs to the technical field of plant genetic engineering. The gene regulating flavonoid synthesis, the sequence is shown in SEQ ID NO: 1, which is derived from common tobacco, named NtMYB27, the NtMYB27 gene is 1240bp in length, contains 3 introns and 4 exons, and its coding region is complete 597 bp long. The present invention finds that the NtMYB27 transcription factor can inhibit the synthesis of flavonols in leaves through cloning analysis of gene sequences, construction of expression vectors, and gene expression detection, and this inhibition is achieved by inhibiting the expression of NtFLS and NtCHS genes of. Therefore, the present invention confirms that NtMYB27 is a negative regulator of plant flavonol synthesis. When the expression of this gene is inhibited or its function is lost, it can significantly increase the content of flavonols in plant leaves, providing a basis for the subsequent use of gene editing technology to enhance plant flavonols. Assays provide clear target genes.

The invention discloses a key gene GbMYB6 for promoting the synthesis of ginkgo flavonoids and its expressed protein, carrier and application. The nucleotide sequence of the key gene GbMYB6 is shown in SEQ ID NO.1, and the amino acid of the expressed protein is The sequence is shown in SEQ ID NO.2. The present invention uses Ginkgo biloba leaves as materials, clones the GbMYB6 gene, constructs an overexpression vector 35S::GbMYB6 containing the gene GbMYB6 by enzyme cleavage, and transfers it into Ginkgo callus and Arabidopsis thaliana, GbMYB6 transgenic Ginkgo callus and transgene The content of flavonoids in Arabidopsis thaliana was significantly increased, which indicated that GbMYB6 can promote the synthesis of flavonoids. Therefore, regulating the expression of GbMYB6 has important application value in improving the medicinal quality of Ginkgo biloba leaves.

The invention discloses a transcription factor gene that regulates the synthesis of plant flavonoids. The transcription factor gene is the MtbZIP10 gene identified from the leguminous plant Medicago truncatula, and its gene sequence is shown in SEQ ID NO.1. Moreover, the present invention discloses the function of the gene and its application. The advantage of the present invention is that the transcription factor gene that can positively regulate the synthesis of flavonoid compounds is cloned from the leguminous plant Medicago truncatula, and its function is systematically identified, and it is found that the gene can activate the biosynthesis pathway of flavonoids Multiple genes that increase the content of flavonoid compounds.

Disclosed are a method for controlling flavonoid synthesis in a chrysanthemum plant or non-chrysanthemum plant by genetic recombination technology using a transcriptional regulatory region useful for altering flower color, a method for modifying anthocyanins, a method for producing a chrysanthemum plant or non-chrysanthemum plant containing modified anthocyanins in the petals thereof, and a chrysanthemum plant or non-chrysanthemum plant, progeny thereof, or vegetatively propagated products, part or tissue thereof transformed with the regulatory region. In the method according to the present invention, an expression vector or expression cassette containing a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase gene, such as a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1, or a transcriptional regulatory region of pansy F3′5′H gene, such as the nucleotide sequence indicated in SEQ ID NO. 15, is used.

The invention provides a chalcone isomerase gene of asparaguses, a protein encoded by the chalcone isomerase gene and application of the chalcone isomerase gene. The CDS sequence of the chalcone isomerase gene AoCHI1 is shown as SEQ ID NO: 1; the amino acid sequence of the protein encoded by the gene is represented by SEQ ID NO: 2. The chalcone isomerase gene AoCHI1 is cloned from the asparagus for the first time, and is one of key genes for synthesizing plant flavonoids; by the adoption of a method of gene engineering, the gene AoCHI1 is transferred into a target plant, so that the total flavonoids content of a transgenic plant can be increased, to improve the plant quality by using a gene engineering technology in the future; furthermore, an important theoretical basis is provided for obtaining medicines or food with high oxidization resistance, and the chalcone isomerase gene has a wide application prospect and high economic value.

The invention belongs to the technical field of genetic engineering application and particularly relates to application of an Arabidopsis thaliana TT4 gene (Arabidopsis thaliana genome code At5g13930) to culture of new varieties of salt-resistant plants. The TT4 gene with a CDS length being 1188bp encodes proteins of 395 amino acids and is related to seed activity, and the gene enables release of Arabidopsis thaliana seed activity after genetic mutation, namely the gene is capable of enhancing seed activity to accelerate seed germination and improve seed germinability after genetic mutation. The TT4 gene mainly serves as a first specific enzyme in a flavonoid synthesis route and participates in regulation of development of seed coats and seed coat pigments of Arabidopsis thaliana. According to researches of the TT4 gene in simulated dry or high-salinity adverse situations, plants under specific regulation of the gene has an osmotic stress reaction to salts and the like, and after silencing of the gene, mutant plants show favorable salt resistance at an initial germination stage.

The invention relates to a gene for regulating flavonoid synthesis, its encoded protein and its application, and belongs to the technical field of plant genetic engineering. The gene that regulates the synthesis of flavonoids, the gene sequence is shown in SEQ ID NO: 1, the gene is derived from common tobacco, named NtMYB59, the full length of the NtMYB59 gene is 9552bp, including 10 introns and 11 exons, which encodes The total length of the region is 1251bp. The present invention finds that the NtMYB59 transcription factor can inhibit the synthesis of flavonoids in leaves through cloning analysis of gene sequences, construction of expression vectors, and gene expression detection, and this inhibition is achieved by inhibiting the expression of NtFLS and NtMYB12 genes . The invention can significantly change the content of flavonoid substances in plant leaves by changing the expression level of NtMYB59 gene, and provides theoretical basis and target genes for improving crop quality.

The invention provides an oxymethyltransferase involved in the synthesis of citrus peel flavonoids and its coding gene and application. The expression level of CitOMT1 is positively correlated with the accumulation of polymethoxylated flavonoids during the fruit development of Citrus reticulata cv. Suavissima; The in vitro enzyme activity test shows that the oxygen methyltransferase can be used in catalyzing the specific site methylation of flavonoids, especially can catalyze quercetin to generate quercetin 3', 4' dimethyl ester; catalyze eriodiction to generate hesperetin and homo eriodictyol, catalyze baicalein to generate saflavone A; catalyze 7,8-dihydroxyflavone to generate 7-hydroxy-8-methoxyflavone . The enzyme activity reaction conditions are simple, and no additional metal ions need to be added, so it has high application value and research prospect.

The invention discloses a key gene GbMYB4 that regulates the synthesis of ginkgo flavonoids and its expressed protein, carrier and application. The nucleotide sequence of the key gene GbMYB4 is shown in SEQ ID NO.1, and the amino acid of the expressed protein is The sequence is shown in SEQ ID NO.2. The present invention uses Ginkgo leaves as materials, clones the GbMYB4 gene, constructs an overexpression vector containing the gene GbMYB4 and transfers it into Ginkgo callus and Arabidopsis thaliana, and the flavonoids in the GbMYB4 transgenic Ginkgo callus and transgenic Arabidopsis of the present invention The contents of the flavonoids were significantly reduced, which indicates that GbMYB4 can inhibit the synthesis of flavonoids, negatively regulate flavonoids, and cooperate with genes that promote flavonoid synthesis to effectively control the synthesis of flavonoids such as Ginkgo biloba, thus regulating the expression of GbMYB4 It has important application value in improving the medicinal quality of Ginkgo biloba leaves.