High-temperature-resistant coloring early selection method for early-medium-maturing apple hybrid seedlings

A high-temperature-resistant apple hybrid technology, applied in biochemical equipment and methods, color/spectral characteristic measurement, material separation, etc., can solve problems such as poor coloring, intolerance to storage and transportation, and poor quality, so as to improve breeding efficiency and reduce Quantity effect

Pending Publication Date: 2021-12-03
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently cultivated early and mid-maturing apple varieties have problems such as poor coloring at high temp...

Method used

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  • High-temperature-resistant coloring early selection method for early-medium-maturing apple hybrid seedlings
  • High-temperature-resistant coloring early selection method for early-medium-maturing apple hybrid seedlings

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Embodiment 1

[0075] A method for early selection of high-temperature-resistant coloring of early-middle-maturing apple hybrid seedlings, comprising:

[0076] S1: Field selection of hybrid seedlings, investigation of biological morphology of hybrid seedlings in the field, mainly removing seedlings with serious diseases and deformed growth, and selecting healthy plants.

[0077] S2: Selection of experimental materials. The functional leaves growing on annual branches were selected as test materials.

[0078] S3: Experimental material treatment. The petioles of the selected functional leaves were wrapped with sterile cotton balls, sprayed with water to keep them moisturized, and the leaves were treated in a light incubator at 40°C and 5000LX ordinary light for 12 hours.

[0079] S4: detection and analysis, the detection and analysis of metabolites, hormone levels and expression levels of coloring-related genes are carried out on the treated experimental materials.

[0080] S5: Early selectio...

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Abstract

The invention discloses a high-temperature-resistant coloring early selection method for early-medium-maturing apple hybrid seedlings, and belongs to the technical field of forestry planting. The method comprises the following steps of: selecting hybrid seedling fields; selecting and treating experimental materials; and screening plants with increased metabolites, increased hormone levels, melatonin synthesis related genes, MYB transcription factors and up-regulated F3 gene expression in flavonoid synthesis pathways in samples as selection objects. Early selection and pre-selection of the hybrid seedlings are carried out by utilizing the correlation between fruits and leaves, so that bad types are eliminated, the number of selected plants is reduced, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of forestry planting, and more specifically relates to an early selection method for high temperature-resistant coloring of early and mid-maturing apple hybrid seedlings. Background technique [0002] Apple is one of the main fruit trees in the world. my country's apple production and cultivation area account for more than half of the world's total. The process of apple cultivation is often accompanied by various adversity stresses, which have a certain impact on the growth and development, yield and quality of apples. [0003] The fruit ripening period of early and mid-maturing apples is in the high temperature period in summer, which leads to poor fruit coloring and low quality, which plagues the development of early and mid-maturing apple cultivation. [0004] Breeding early and mid-maturing varieties with high temperature resistance and excellent coloring is of great significance to the healthy and sust...

Claims

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Application Information

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IPC IPC(8): C12Q1/30C12Q1/28C12Q1/26C12Q1/6851G01N21/31G01N30/02G01N30/72G01N33/68
CPCC12Q1/30C12Q1/28C12Q1/26C12Q1/6851G01N21/31G01N30/02G01N30/72G01N33/6839G01N2333/908G01N2333/90283C12Q2521/107C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 何平常源升李林光王海波王森何晓文
Owner SHANDONG INST OF POMOLOGY
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