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Gene for regulating flavonoid synthesis and ultraviolet resistance of plants and application of gene

A flavonoid, plant technology, applied in the fields of botany and molecular biology, can solve the problems of low flavonoid accumulation and low MYB gene expression

Active Publication Date: 2020-03-20
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the research on UV-B-induced flavonoid accumulation and resistance to UV stress is not very clear, at least the following issues need to be resolved: 1. In the absence of UV-B, MYB gene expression is low and flavonoid accumulation is low, so the plant How to inhibit the expression of MYB under normal growth state? Positive regulatory factors have been found to promote MYB gene expression, are there other factors that regulate MYB gene expression? 2. How do plants balance normal growth and development and resist UV stress? Therefore, there is an urgent need to study these issues in this field. Finding more genes that regulate MYB is conducive to finely directional regulation of the accumulation of flavonoids; elucidating the molecular mechanism of plant balanced growth and development and resistance to UV stress, which will help to breed plants that are both resistant to UV stress. High-quality varieties that take into account the yield

Method used

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  • Gene for regulating flavonoid synthesis and ultraviolet resistance of plants and application of gene
  • Gene for regulating flavonoid synthesis and ultraviolet resistance of plants and application of gene
  • Gene for regulating flavonoid synthesis and ultraviolet resistance of plants and application of gene

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Experimental program
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Effect test

Embodiment 1

[0110] Embodiment 1, plant hormone brassinosterol (BR) negatively regulates UV stress resistance

[0111] In this example, it is mainly studied whether BR regulates the resistance of plants to ultraviolet stress. The present inventors observed the physiological phenotypes of BR synthesis mutants or signal transduction mutants under ultraviolet stress conditions. from figure 1 In a, it can be seen that the BR synthetic mutant det2, the BR receptor mutant bri1, and the key transcription factor BES1 mutant BES1-RNAi in the BR pathway are more resistant to UV stress than wild-type Col. UV stress has been reported to damage the photosynthetic complex and directly inhibit the photosynthetic efficiency, so the inventors measured the maximum photochemical quantum yield (Fv / Fm) of photosystem II as an index reflecting the degree of plant stress. The results of Fv / Fm were consistent with the stress phenotype, and the photosynthetic performance of BR synthesis or signal transduction m...

Embodiment 2

[0112] Example 2, BES1 inhibits the synthesis of flavonols by inhibiting the expression of MYBs genes

[0113] The accumulation of flavonoids is an important mechanism for plants to resist UV stress. The flavonoid synthesis pathway involves a series of enzymatic reactions, including CHS (chalcone synthase), CHI (chalcone isomerase) and so on. Three MYB transcription factors (MYB11, MYB12, MYB111) control flavonol synthesis by activating the expression of flavonol synthesis genes (CHS, CHI, etc.). The inventors used real-time quantitative PCR (q-RT-PCR) to detect whether BES1 regulates the expression of MYBs genes (MYB11, MYB12, MYB111), and found that MYBs was upregulated in BES1-RNAi compared to Col, and in bes1-D- Down-regulated in OX ( figure 2 a-b). The inventor further uses high-performance liquid chromatography (HPLC) to detect the flavonoid level in the plant, extracts the phenylpropanoid compound, and analyzes it with HPLC, such as figure 2 g, Wild-type Col has cl...

Embodiment 3

[0114] Example 3, BES1 directly binds to MYBs promoter in response to BR signal

[0115] BES1 can inhibit the expression of MYBs gene to control the synthesis of flavonols, so can BES1 directly bind to the promoter of MYBs gene? Then the present inventor just analyzes MYBs gene promoter (MYB11 promoter sequence such as Gene ID: 825435 initiation codon upstream 2Kb region, MYB12 promoter sequence such as Gene ID: 819359 initiation codon upstream 2Kb region, MYB111 promoter promoter Sequence such as Gene ID: 834993 upstream of the start codon 2Kb region), found that each MYB gene promoter has multiple G-box elements, significantly more than BRRE elements, which suggests that BES1 may bind to the G-box, thereby inhibiting MYBs transcription. In order to prove this hypothesis, the present inventors performed a gel migration assay (EMSA). like image 3 a, BES1 can indeed bind to MYB11, MYB12, and MYB111 promoters, but cannot bind to the MYB12 promoter with a G-box mutation.

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Abstract

The invention relates to a gene for regulating flavonoid synthesis and ultraviolet resistance of plants and application of the gene. Effects and molecular mechanisms of brassinosteroid signaling pathway on the flavonoid synthesis in plants and resistance of plants to ultraviolet rays are studied and revealed for the first time. The brassinosteroid signaling pathway, particularly transcription factor BES1 in the brassinosteroid signaling pathway, can negatively regulate MYB transcription factor, regulate the flavonoid synthesis in plants on the basis, and further regulate the resistance of plants to ultraviolet rays.

Description

technical field [0001] The invention belongs to the fields of botany and molecular biology, and more specifically, the invention relates to genes regulating plant flavonoid synthesis and ultraviolet resistance and applications thereof. Background technique [0002] In plant photosynthesis, the main spectral regions that can be used are the red light part with a wavelength of 640-660nm and the blue light part with a wavelength of 400-500nm. However, ultraviolet rays have no effect on plant photosynthesis. In the prior art, it is also believed that too much ultraviolet radiation will affect the photosynthesis efficiency of plants. In the process of plant evolution, some mechanisms for resisting UV stress have also been acquired in the body. [0003] Ultraviolet UV-B (280-315nm) is a part of sunlight, which can partially reach the earth's surface and has an important impact on plant growth and development. UV-B can act as a light signal to regulate plant growth and developmen...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H6/20A01H6/46C12Q1/6895
CPCC12N15/8243C12N15/8271C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 刘宏涛梁通史辰
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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