MYB transcription factor separated from purple white clove and coding gene and application thereof

Abstract

The invention discloses an MYB transcription factor separated from a purple white clove and a coding gene and application thereof. The purple V-marking white clove serves as the material, by means ofan RNA-Seq technology, the transcription factor TrMYB308 related to flavonoid synthesis is screened out, on the basis, a TrMYB308 gene is cloned, the nucleotide sequence is shown in SEQ ID No.2. The TrMYB308 gene is induced by JA and expressed in all tissues of the purple white clove. It is found from the results obtained when the TrMYB308 gene is subjected to heterologous expression in hairy roots of tartartary buckwheat that the expression quantity of part of key enzyme genes of a flavonoid metabolism pathway in transgenic roots is obviously increased, the total flavonoid content of the transgenic roots is obviously higher than that of a control group, and it is indicated that the TrMYB308 gene participates in biosynthesis regulation of secondary metabolism of flavonoid in plants. Accordingly, the theoretical basis and application basis are provided for buckwheat flavonoid synthetic molecular mechanism exploration, buckwheat quality improvement and the like.

Description

technical field [0001] The present invention relates to transcription factors, in particular to the MYB type transcription factors and their coding genes related to the biosynthesis of plant flavonoids isolated from P. MYB transcription factors and their application fields. Background technique [0002] Jasmonic acids (JAs) are a new class of plant hormones that are ubiquitous in plants. Methyl jasmonate (MeJA) is an active molecular form of JAs. JAs have multiple functions. On the one hand, they can regulate specific primary metabolic pathways to provide necessary substrates for secondary metabolism; on the other hand, they can regulate secondary metabolic processes and synthesize secondary metabolites in response to biotic and abiotic stresses, such as mechanical stresses. Impairs insect defenses against pathogenic bacteria. Previous studies have shown that jasmonic acids (JAs) regulate the expression of key enzyme genes related to plant secondary metabolism by activatin...

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Problems solved by technology

Plant gene function is generally studied extensively in Arabidopsis, but due to its poor agronomic traits, the application value of research results in Arabidopsis is very limited

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